Western blot abreview for Anti-HOXA1 antibody

Good
Abreviews
Application
Western blot
Sample
Human Cell lysate - whole cell (Renal clear cell carcinoma line 769-P)
Loading amount
12 µg
Specification
Renal clear cell carcinoma line 769-P
Gel Running Conditions
Reduced Denaturing (10)
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C

Other product details

Dilution
1/1000
Incubation time
1 hour(s) and 0 minute(s) · Temperature: 22°C · Diluent: PBST

Secondary antibody

Dilution
1/5000

Detection

Detection method
ECL
Exposure
1 minute(s) and 0 second(s)
Bands
Specific: 39 kDa Non-specific: 73, 160 kDa
Positive control
(1) 769-P whole cell lysate (derived from a Renal Clear Cell Carcinoma)

Additional data

Additional Notes
The predicted MW of HOXA1 is 36.6kDa

Abcam user community

Verified customer

Submitted Apr 10 2008

Sign up