Overview

  • Product name

  • Description

    Goat polyclonal to HOXA10
  • Host species

    Goat
  • Tested applications

    Suitable for: WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Horse, Chicken, Cow, Pig, Chimpanzee, Monkey, Gorilla
  • Immunogen

    Synthetic peptide corresponding to Human HOXA10 aa 152-165 (internal sequence). (NP_061824.3).
    Sequence:

    PQATSCSFAQNIKE


    Database link: P31260

  • Positive control

    • Human brain cerebellum and testis tissues: Human skeletal muscle lysates.

Properties

Applications

Our Abpromise guarantee covers the use of ab191470 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.2 - 0.6 µg/ml. Detects a band of approximately 38 kDa (predicted molecular weight: 42 kDa).
IHC-P Use a concentration of 5 µg/ml.

Target

Images

  • Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human testis tissue labeling HOXA10 with ab191470 at 5 µg/ml.

  • Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human brain cerebellum tissue labeling HOXA10 with ab191470 at 5 µg/ml.

  • Anti-HOXA10 antibody (ab191470) at 0.2 µg/ml + Human Skeletal Muscle lysate in RIPA buffer at 35 µg

    Developed using the ECL technique.

    Predicted band size: 42 kDa
    Observed band size: 38 kDa
    why is the actual band size different from the predicted?



    Primary incubation was 1 hour.

References

This product has been referenced in:

  • Yang QS  et al. Long noncoding RNA LINC00483/microRNA-144 regulates radiosensitivity and epithelial-mesenchymal transition in lung adenocarcinoma by interacting with HOXA10. J Cell Physiol 234:11805-11821 (2019). Read more (PubMed: 30714135) »
  • Guo S  et al. GnRH agonist improves pregnancy outcome in mice with induced adenomyosis by restoring endometrial receptivity. Drug Des Devel Ther 12:1621-1631 (2018). Read more (PubMed: 29922037) »
See all 4 Publications for this product

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