Overview

  • Product name

    Anti-HOXA11 antibody
  • Description

    Rabbit polyclonal to HOXA11
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, ELISA, ICC/IFmore details
  • Species reactivity

    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide derived from internal sequence of human HOXA11.

  • Positive control

    • Extracts from RAW264.7 cells.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • Storage buffer

    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituents: 50% Glycerol, 0.87% Sodium chloride, PBS

    Without Mg2+ and Ca2+
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab72591 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Predicted molecular weight: 35 kDa.
ELISA 1/5000.
ICC/IF 1/500 - 1/1000.

Target

  • Function

    Sequence-specific transcription factor which is part of a developmental regulatory system that provides cells with specific positional identities on the anterior-posterior axis.
  • Involvement in disease

    Radioulnar synostosis with amegakaryocytic thrombocytopenia
  • Sequence similarities

    Belongs to the Abd-B homeobox family.
    Contains 1 homeobox DNA-binding domain.
  • Cellular localization

    Nucleus.
  • Information by UniProt
  • Database links

  • Alternative names

    • Homeo box 1I antibody
    • Homeo box A11 antibody
    • Homeobox A11 antibody
    • Homeobox protein Hox A11 antibody
    • Homeobox protein Hox-1I antibody
    • Homeobox protein Hox-A11 antibody
    • Homeobox protein HOXA11 antibody
    • Hox 1I antibody
    • HOX1 antibody
    • HOX1I antibody
    • HOXA11 antibody
    • HXA11_HUMAN antibody
    see all

Images

  • All lanes : Anti-HOXA11 antibody (ab72591) at 1/500 dilution

    Lane 1 : Extracts from RAW264.7 cells
    Lane 2 : Extracts from RAW264.7 cells with immunising peptide at 5 µg

    Lysates/proteins at 5 µg per lane.

    Predicted band size: 35 kDa
    Observed band size: 30 kDa
    why is the actual band size different from the predicted?

  • Immunofluorescence analysis of HUVEC cells, using ab72591, at 1/500 dilution, in the presence (right panel) or absence (left panel) of the immunising peptide.

References

This product has been referenced in:

See 1 Publication for this product

Customer reviews and Q&As

1-2 of 2 Q&A

Answer

Thank you for contacting us.

I am sorry that this antibody did not perform as stated on the datasheet. If payment has already been made on the original order and you wish to receive a refund, please ask your purchasing department to contact our accounting department so that we may gather the correct information needed for the refund. To avoid confusion, please ensure your accounts department is aware of how the credit note is being used.

Our accounting department can be contacted by email at us.credits@abcam.com or by telephone using the information at the Contact Us link in the top right corner of our website. Please refer to the credit note ID in any correspondence with our accounting department.

I hope this experience will not prevent you from purchasing other products from us in the future. Our Scientific Support team is always at your service, should you require further expert advice.

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Answer

Thank you for contacting Abcam regarding ab72591.


I am sorry that you have been experiencing difficulties with this antibody in WB. I have reviewed your protocol and image and I agree that this product is not working as stated on the datasheet. I am happy to offer a replacement or credit. Please let me know which you would prefer. Would you also include your order number with your reply?


I look forward to your reply so that I may assist you further. Please do not hesitate to contact us if you have any additional questions.

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