Key features and details
- Rabbit polyclonal to HOXA2
- Suitable for: IHC-P, WB
- Reacts with: Mouse, Human
- Isotype: IgG
Product nameAnti-HOXA2 antibody
See all HOXA2 primary antibodies
DescriptionRabbit polyclonal to HOXA2
Tested applicationsSuitable for: IHC-P, WBmore details
Species reactivityReacts with: Mouse, Human
Predicted to work with: Cow
- IHC-P: Human liver cancer and tonsil tissues. WB: Jurkat whole cell lysate; Mouse liver, kidney and stomach lysates.
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We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Constituents: 50% Glycerol (glycerin, glycerine), PBS, 0.03% Proclin 300
Concentration information loading...
PurityProtein G purified
Purification notesPurity > 95%.
Our Abpromise guarantee covers the use of ab229960 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/20 - 1/200.|
|WB||1/2000 - 1/5000. Detects a band of approximately 42 kDa (predicted molecular weight: 41 kDa).|
FunctionSequence-specific transcription factor which is part of a developmental regulatory system that provides cells with specific positional identities on the anterior-posterior axis.
Involvement in diseaseDefects in HOXA2 are a cause of microtia hearing impairment and cleft palate (MHICP) [MIM:612290]. Microtia is a congenital deformity of the outer ear and occurs in approximately one in 8'000-10'000 births. It is characterized by a small, abnormally shaped outer ear. It can be unilateral or bilateral. Syndromic forms of microtia occur in conjunction with other abnormalities. The most common associated malformations is the cleft palate, a congenital fissure of the soft and/or hard palate due to faulty fusion. Defects in HOXA2 are a cause of autosomal-recessive bilateral microtia, mixed symmetrical severe to profound hearing impairment and partial cleft palate.
Sequence similaritiesBelongs to the Antp homeobox family. Proboscipedia subfamily.
Contains 1 homeobox DNA-binding domain.
- Information by UniProt
- AI324701 antibody
- homeo box A2 antibody
- homeobox A2 antibody
Paraffin-embedded human liver cancer tissue stained for HOXA2 using ab229960 at 1/100 dilution in immunohistochemical analysis.
Paraffin-embedded human tonsil tissue stained for HOXA2 using ab229960 at 1/100 dilution in immunohistochemical analysis.
All lanes : Anti-HOXA2 antibody (ab229960) at 1/2000 dilution
Lane 1 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate
Lane 2 : Mouse liver lysate
Lane 3 : Mouse kidney lysate
Lane 4 : Mouse stomach lysate
All lanes : Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 41 kDa
Observed band size: 42 kDa why is the actual band size different from the predicted?
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab229960 has not yet been referenced specifically in any publications.