Anti-HOXA2 antibody (ab229960)
Key features and details
- Rabbit polyclonal to HOXA2
- Suitable for: IHC-P, WB
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
-
Product name
Anti-HOXA2 antibody
See all HOXA2 primary antibodies -
Description
Rabbit polyclonal to HOXA2 -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WBmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Cow -
Immunogen
Recombinant fragment corresponding to Human HOXA2 aa 1-150.
Database link: O43364 -
Positive control
- IHC-P: Human liver cancer and tonsil tissues. WB: Jurkat whole cell lysate; Mouse liver, kidney and stomach lysates.
-
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol (glycerin, glycerine), PBS -
Concentration information loading...
-
Purity
Protein G purified -
Purification notes
Purity > 95%. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
-
Compatible Secondaries
-
Isotype control
-
Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab229960 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
IHC-P |
1/20 - 1/200.
|
|
WB |
1/2000 - 1/5000. Detects a band of approximately 42 kDa (predicted molecular weight: 41 kDa).
|
Notes |
---|
IHC-P
1/20 - 1/200. |
WB
1/2000 - 1/5000. Detects a band of approximately 42 kDa (predicted molecular weight: 41 kDa). |
Target
-
Function
Sequence-specific transcription factor which is part of a developmental regulatory system that provides cells with specific positional identities on the anterior-posterior axis. -
Involvement in disease
Defects in HOXA2 are a cause of microtia hearing impairment and cleft palate (MHICP) [MIM:612290]. Microtia is a congenital deformity of the outer ear and occurs in approximately one in 8'000-10'000 births. It is characterized by a small, abnormally shaped outer ear. It can be unilateral or bilateral. Syndromic forms of microtia occur in conjunction with other abnormalities. The most common associated malformations is the cleft palate, a congenital fissure of the soft and/or hard palate due to faulty fusion. Defects in HOXA2 are a cause of autosomal-recessive bilateral microtia, mixed symmetrical severe to profound hearing impairment and partial cleft palate. -
Sequence similarities
Belongs to the Antp homeobox family. Proboscipedia subfamily.
Contains 1 homeobox DNA-binding domain. -
Cellular localization
Nucleus. - Information by UniProt
-
Database links
- Entrez Gene: 524150 Cow
- Entrez Gene: 3199 Human
- Entrez Gene: 15399 Mouse
- Omim: 604685 Human
- SwissProt: Q0VCS4 Cow
- SwissProt: O43364 Human
- SwissProt: P31245 Mouse
- Unigene: 445239 Human
see all -
Alternative names
- AI324701 antibody
- homeo box A2 antibody
- homeobox A2 antibody
see all
Images
-
Paraffin-embedded human liver cancer tissue stained for HOXA2 using ab229960 at 1/100 dilution in immunohistochemical analysis.
-
Paraffin-embedded human tonsil tissue stained for HOXA2 using ab229960 at 1/100 dilution in immunohistochemical analysis.
-
All lanes : Anti-HOXA2 antibody (ab229960) at 1/2000 dilution
Lane 1 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate
Lane 2 : Mouse liver lysate
Lane 3 : Mouse kidney lysate
Lane 4 : Mouse stomach lysate
Secondary
All lanes : Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 41 kDa
Observed band size: 42 kDa why is the actual band size different from the predicted?
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
-
SDS download
-
Datasheet download
References (3)
ab229960 has been referenced in 3 publications.
- Ren P et al. A novel hypoxia-driven gene signature that can predict the prognosis and drug resistance of gliomas. Front Genet 13:976356 (2022). PubMed: 36118887
- Cheng F et al. Long Non-Coding RNA UBA6-AS1 Promotes the Malignant Properties of Glioblastoma by Competitively Binding to microRNA-760 and Enhancing Homeobox A2 Expression. Cancer Manag Res 13:379-392 (2021). PubMed: 33469379
- Wang Z et al. Effects of Doxorubicin on Heme Biosynthesis and Metabolism in Cardiomyocyte. Arq Bras Cardiol 116:315-322 (2021). PubMed: 33656082