Overview

  • Product name
  • Description
    Rabbit polyclonal to HOXA3
  • Host species
    Rabbit
  • Tested applications
    Suitable for: ELISA, WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Dog, Zebrafish
  • Immunogen

    A region within synthetic peptide: MDYGGAGPLG SGHHHGPGPG EPHPTYTDLT GHHPSQGRIQ EAPKLTHL, corresponding to C terminal amino acids 396-443 of Human HOXA3

  • Positive control
    • HepG2 Cell Lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab28771 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA 1/62500.
WB Use a concentration of 2.5 µg/ml. Detects a band of approximately 48 kDa (predicted molecular weight: 46 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

Target

Images

  • Anti-HOXA3 antibody (ab28771) at 2.5 µg/ml + HepG2 cell lysate at 10 µg/ml

    Secondary
    HRP conjugated anti Rabbit IgG at 1/50000 dilution

    Predicted band size: 46 kDa
    Observed band size: 48 kDa
    why is the actual band size different from the predicted?

References

ab28771 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Answer

We currently have over forty products against these HOX targets. These product will be specific to the HOX protein which they are raised against, whether A1, A2, A3 etc. We currently do not carry an anti-HOX antibody which will recognize a number of HOX proteins. While we do not test for and cannot guarantee efficacy of our antibodies in EMSA super shift, I have reviewed the catalog for products which would be most suitable for this assay. Generally we would want to look for monoclonal antibodies which have been show to recognize the protein in a native form. For targets which do not have monoclonal antibodies available, I have chosen the best available alternative for you. This list is included in the spreadsheet which I have attached to this email.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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