Overview

  • Product name

  • Description

    Goat polyclonal to HOXA5
  • Host species

    Goat
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human, Pig
    Predicted to work with: Cow
  • Immunogen

    Synthetic peptide corresponding to Human HOXA5 aa 83-92 (internal sequence) (Cysteine residue). (NP_061975.2).
    Sequence:

    EPRYSQPATS


    Database link: P20719

  • Positive control

    • WB: Human, mouse, pig and rat kidney lysates.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.30
    Preservative: 0.02% Sodium azide
    Constituents: 0.5% BSA, 99% Tris buffered saline
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    ab180229 was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab180229 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 3 µg/ml. Detects a band of approximately 28 kDa (predicted molecular weight: 29 kDa).

Target

Images

  • All lanes : Anti-HOXA5 antibody (ab180229) at 1 µg/ml

    Lane 1 : Human kidney lysate (in RIPA buffer)
    Lane 2 : Mouse kidney lysate (in RIPA buffer)
    Lane 3 : Rat kidney lysate (in RIPA buffer)

    Lysates/proteins at 35 µg per lane.

    Developed using the ECL technique.

    Predicted band size: 29 kDa
    Observed band size: 28 kDa
    why is the actual band size different from the predicted?



    Primary incubation was 1 hour.

  • Anti-HOXA5 antibody (ab180229) at 1 µg/ml + Pig kidney lysate (in RIPA buffer) at 35 µg

    Developed using the ECL technique.

    Predicted band size: 29 kDa
    Observed band size: 28 kDa why is the actual band size different from the predicted?



    Primary incubation was 1 hour.

References

ab180229 has not yet been referenced specifically in any publications.

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There are currently no Customer reviews or Questions for ab180229.
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