Overview

  • Product name

    Anti-HOXA9 antibody - C-terminal
    See all HOXA9 primary antibodies
  • Description

    Rabbit polyclonal to HOXA9 - C-terminal
  • Host species

    Rabbit
  • Tested applications

    Suitable for: Flow Cyt, WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Chicken, Guinea pig
  • Immunogen

    Synthetic peptide within Human HOXA9 aa 245-272 (C terminal) conjugated to Keyhole Limpet Haemocyanin (KLH). The exact sequence is proprietary.
    Sequence:

    LTERQVKIWFQNRRMKMKKINKDRAKDE


    Database link: P31269

  • Positive control

    • Recombinant Human HOXA9 protein (denatured) (ab180306) can be used as a positive control in WB. Human prostate tissue; A375 cells; A2058 cell lysate.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.09% Sodium azide
    Constituent: 99% PBS
  • Concentration information loading...
  • Purity

    Ammonium Sulphate Precipitation
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab191178 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt 1/10 - 1/50.

ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.

 

WB 1/1000. Predicted molecular weight: 30 kDa.
IHC-P 1/75.

Target

  • Function

    Sequence-specific transcription factor which is part of a developmental regulatory system that provides cells with specific positional identities on the anterior-posterior axis.
  • Involvement in disease

    Note=A chromosomal aberration involving HOXA9 is found in a form of acute myeloid leukemia. Translocation t(7;11)(p15;p15) with NUP98.
    Note=A chromosomal aberration involving HOXA9 may contribute to disease progression in chronic myeloid leukemia. Translocation t(7;17)(p15;q23) with MSI2.
  • Sequence similarities

    Belongs to the Abd-B homeobox family.
    Contains 1 homeobox DNA-binding domain.
  • Cellular localization

    Nucleus.
  • Information by UniProt
  • Database links

  • Form

    HOXA9 is a transcription factor with a central role in both haemopoiesis and leukaemia. High levels of HOXA9 expression in haemopoietic cells is a characteristic feature of acute myeloid leukaemia (AML), and may be sufficient to cause this disease. Overexpression of Hoxa 9 markedly expands hematopoietic stem cells.
  • Alternative names

    • ABD-B antibody
    • Abd-B, drosophila, homolog of antibody
    • D6a9 antibody
    • Homeobox 1G antibody
    • Homeobox A9 antibody
    • Homeobox protein Hox-1.7 antibody
    • Homeobox protein Hox-1G antibody
    • Homeobox protein Hox-A9 antibody
    • Homeodomain protein HOXA9 antibody
    • Hox-1.7 antibody
    • Hox-1.7, mouse, homolog of antibody
    • HOX1 antibody
    • Hox1.7 antibody
    • Hox1G antibody
    • Hox1r5 antibody
    • Hoxa-9 antibody
    • Hoxa7 antibody
    • HOXA9 antibody
    • HOXA9/MSI2 fusion gene, included antibody
    • HOXA9/NUP98 fusion gene, included antibody
    • HXA9_HUMAN antibody
    • MGC1934 antibody
    see all

Images

  • Anti-HOXA9 antibody - C-terminal (ab191178) at 1/1000 dilution + A2058 cell lysate at 35 µg

    Predicted band size: 30 kDa

  • Flow cytometric analysis of A375 cells labeling HOXA9 with ab191178 at 1/10 dilution (right histogram) compared to negative control cells (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

  • Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human prostate tissue labeling HOXA9 with ab191178 at 1/75 dilution.

References

This product has been referenced in:

  • Zhang ZF  et al. MicroRNA-182 downregulates Wnt/ß-catenin signaling, inhibits proliferation, and promotes apoptosis in human osteosarcoma cells by targeting HOXA9. Oncotarget 8:101345-101361 (2017). Read more (PubMed: 29254169) »
  • Morgan R  et al. HOX transcription factors are potential targets and markers in malignant mesothelioma. BMC Cancer 16:85 (2016). Read more (PubMed: 26867567) »
See all 2 Publications for this product

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