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RecombinantRabMAb

Recombinant Anti-HOXA9 antibody [EPR3655(2)] (ab140631)

  • Datasheet
Reviews (1) Submit a question References (12)

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Western blot - Anti-HOXA9 antibody [EPR3655(2)] (ab140631)
  • Western blot - Anti-HOXA9 antibody [EPR3655(2)] (ab140631)
  • Anti-HOXA9 antibody [EPR3655(2)] (ab140631)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR3655(2)] to HOXA9
  • Suitable for: WB
  • Reacts with: Human

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Overview

  • Product name

    Anti-HOXA9 antibody [EPR3655(2)]
    See all HOXA9 primary antibodies
  • Description

    Rabbit monoclonal [EPR3655(2)] to HOXA9
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
    Unsuitable for: Flow Cyt,ICC or IHC-P
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human HOXA9 aa 250 to the C-terminus (C terminal). The exact sequence is proprietary.

  • Positive control

    • HepG2, Human thymus, U937, SW480 and 293T lysates; HepG2 cells.
  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C.
  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituents: 40% Glycerol (glycerin, glycerine), 0.05% BSA, 59% PBS
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR3655(2)
  • Isotype

    IgG
  • Research areas

    • Stem Cells
    • Endothelial Progenitors
    • Endothelial Markers

Associated products

  • Alternative Versions

    • Anti-HOXA9 antibody [EPR3655(2)] - BSA and Azide free (ab248915)
  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • Positive Controls

    • SW480 whole cell lysate (ab3957)
  • Recombinant Protein

    • Recombinant Human HOXA9 protein (ab152453)

Applications

Our Abpromise guarantee covers the use of ab140631 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 30 kDa.
  • Application notes
    Is unsuitable for Flow Cyt,ICC or IHC-P.
  • Target

    • Function

      Sequence-specific transcription factor which is part of a developmental regulatory system that provides cells with specific positional identities on the anterior-posterior axis.
    • Involvement in disease

      Note=A chromosomal aberration involving HOXA9 is found in a form of acute myeloid leukemia. Translocation t(7;11)(p15;p15) with NUP98.
      Note=A chromosomal aberration involving HOXA9 may contribute to disease progression in chronic myeloid leukemia. Translocation t(7;17)(p15;q23) with MSI2.
    • Sequence similarities

      Belongs to the Abd-B homeobox family.
      Contains 1 homeobox DNA-binding domain.
    • Cellular localization

      Nucleus.
    • Target information above from: UniProt accession P31269 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 3205 Human
      • Omim: 142956 Human
      • SwissProt: P31269 Human
      • Unigene: 659350 Human
      • Form

        HOXA9 is a transcription factor with a central role in both haemopoiesis and leukaemia. High levels of HOXA9 expression in haemopoietic cells is a characteristic feature of acute myeloid leukaemia (AML), and may be sufficient to cause this disease. Overexpression of Hoxa 9 markedly expands hematopoietic stem cells.
      • Alternative names

        • ABD-B antibody
        • Abd-B, drosophila, homolog of antibody
        • D6a9 antibody
        • Homeobox 1G antibody
        • Homeobox A9 antibody
        • Homeobox protein Hox-1.7 antibody
        • Homeobox protein Hox-1G antibody
        • Homeobox protein Hox-A9 antibody
        • Homeodomain protein HOXA9 antibody
        • Hox-1.7 antibody
        • Hox-1.7, mouse, homolog of antibody
        • HOX1 antibody
        • Hox1.7 antibody
        • Hox1G antibody
        • Hox1r5 antibody
        • Hoxa-9 antibody
        • Hoxa7 antibody
        • HOXA9 antibody
        • HOXA9/MSI2 fusion gene, included antibody
        • HOXA9/NUP98 fusion gene, included antibody
        • HXA9_HUMAN antibody
        • MGC1934 antibody
        see all

      Images

      • Western blot - Anti-HOXA9 antibody [EPR3655(2)] (ab140631)
        Western blot - Anti-HOXA9 antibody [EPR3655(2)] (ab140631)
        All lanes : Anti-HOXA9 antibody [EPR3655(2)] (ab140631) at 1/2000 dilution (purified)

        Lane 1 : HepG2 whole cell lysate
        Lane 2 : Human thymus lysate
        Lane 3 : U937 whole cell lysate
        Lane 4 : HEK293 whole cell lysate

        Lysates/proteins at 20 µg per lane.

        Secondary
        All lanes : Anti-rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

        Predicted band size: 30 kDa
        Observed band size: 30 kDa



        Blocking buffer: 5% NFDM/TBST
        Dilution buffer: 5% NFDM/TBST

      • Western blot - Anti-HOXA9 antibody [EPR3655(2)] (ab140631)
        Western blot - Anti-HOXA9 antibody [EPR3655(2)] (ab140631)
        All lanes : Anti-HOXA9 antibody [EPR3655(2)] (ab140631) at 1/1000 dilution (Unpurified)

        Lane 1 : HepG2 cell lysate
        Lane 2 : Human thymus tissue lysate
        Lane 3 : U937 cell lysate
        Lane 4 : SW480 ell lysate
        Lane 5 : 293T cell lysate

        Lysates/proteins at 10 µg per lane.

        Predicted band size: 30 kDa

      • Anti-HOXA9 antibody [EPR3655(2)] (ab140631)
        Anti-HOXA9 antibody [EPR3655(2)] (ab140631)

      Protocols

      • Western blot protocols

      Click here to view the general protocols

      Datasheets and documents

      • Datasheet
    • References (12)

      Publishing research using ab140631? Please let us know so that we can cite the reference in this datasheet.

      ab140631 has been referenced in 12 publications.

      • Li C  et al. Homoharringtonine exhibits potent anti-tumor effect and modulates DNA epigenome in acute myeloid leukemia by targeting SP1/TET1/5hmC. Haematologica 105:148-160 (2020). PubMed: 30975912
      • Nguyen DTT  et al. HyperTRIBE uncovers increased MUSASHI-2 RNA binding activity and differential regulation in leukemic stem cells. Nat Commun 11:2026 (2020). PubMed: 32332729
      • Sun Q  et al. HIF-1a or HOTTIP/CTCF Promotes Head and Neck Squamous Cell Carcinoma Progression and Drug Resistance by Targeting HOXA9. Mol Ther Nucleic Acids 20:164-175 (2020). PubMed: 32169804
      • Han S  et al. HOXA9 Transcriptionally Promotes Apoptosis and Represses Autophagy by Targeting NF-?B in Cutaneous Squamous Cell Carcinoma. Cells 8:N/A (2019). PubMed: 31683603
      • Minuesa G  et al. Small-molecule targeting of MUSASHI RNA-binding activity in acute myeloid leukemia. Nat Commun 10:2691 (2019). PubMed: 31217428
      View all Publications for this product

      Customer reviews and Q&As

      Show All Reviews Q&A
      Submit a review Submit a question

      Immunohistochemistry (PFA perfusion fixed frozen sections) abreview for Anti-HOXA9 antibody [EPR3655(2)]

      Excellent
      Abreviews
      Abreviews
      abreview image
      Application
      Immunohistochemistry (PFA perfusion fixed frozen sections)
      Sample
      Mouse Tissue sections (E11.5 neural tube)
      Antigen retrieval step
      None
      Permeabilization
      Yes - 0.1% Triton-X
      Specification
      E11.5 neural tube
      Blocking step
      Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 25°C
      Fixative
      Paraformaldehyde
      Read More

      Abcam user community

      Verified customer

      Submitted Dec 07 2015

      Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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