Overview

  • Product name
    Anti-HOXA9 antibody [HOX5I043]
    See all HOXA9 primary antibodies
  • Description
    Mouse monoclonal [HOX5I043] to HOXA9
  • Host species
    Mouse
  • Tested applications
    Suitable for: ELISA, WBmore details
  • Species reactivity
    Reacts with: Recombinant fragment
    Predicted to work with: Human
  • Immunogen

    Recombinant Human HOXA9

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.05% Sodium Azide
    Constituents: 1% BSA, PBS, pH 7.4
  • Concentration information loading...
  • Purity
    Protein G purified
  • Purification notes
    ab51236 was purified from culture supernatant of the hybridoma, cultured in a medium containing bovine IgG-depleted (approximately 95%) fetal bovine serum.
  • Clonality
    Monoclonal
  • Clone number
    HOX5I043
  • Isotype
    IgG1
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab51236 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA Use at an assay dependent dilution.
WB Use at an assay dependent dilution. Predicted molecular weight: 30 kDa.

Target

  • Function
    Sequence-specific transcription factor which is part of a developmental regulatory system that provides cells with specific positional identities on the anterior-posterior axis.
  • Involvement in disease
    Note=A chromosomal aberration involving HOXA9 is found in a form of acute myeloid leukemia. Translocation t(7;11)(p15;p15) with NUP98.
    Note=A chromosomal aberration involving HOXA9 may contribute to disease progression in chronic myeloid leukemia. Translocation t(7;17)(p15;q23) with MSI2.
  • Sequence similarities
    Belongs to the Abd-B homeobox family.
    Contains 1 homeobox DNA-binding domain.
  • Cellular localization
    Nucleus.
  • Information by UniProt
  • Database links
  • Form
    HOXA9 is a transcription factor with a central role in both haemopoiesis and leukaemia. High levels of HOXA9 expression in haemopoietic cells is a characteristic feature of acute myeloid leukaemia (AML), and may be sufficient to cause this disease. Overexpression of Hoxa 9 markedly expands hematopoietic stem cells.
  • Alternative names
    • ABD-B antibody
    • Abd-B, drosophila, homolog of antibody
    • D6a9 antibody
    • Homeobox 1G antibody
    • Homeobox A9 antibody
    • Homeobox protein Hox-1.7 antibody
    • Homeobox protein Hox-1G antibody
    • Homeobox protein Hox-A9 antibody
    • Homeodomain protein HOXA9 antibody
    • Hox-1.7 antibody
    • Hox-1.7, mouse, homolog of antibody
    • HOX1 antibody
    • Hox1.7 antibody
    • Hox1G antibody
    • Hox1r5 antibody
    • Hoxa-9 antibody
    • Hoxa7 antibody
    • HOXA9 antibody
    • HOXA9/MSI2 fusion gene, included antibody
    • HOXA9/NUP98 fusion gene, included antibody
    • HXA9_HUMAN antibody
    • MGC1934 antibody
    see all

Images

  • Anti-HOXA9 antibody [HOX5I043] (ab51236)
    Predicted band size: 30 kDa
    Observed band size: 32 kDa
    why is the actual band size different from the predicted?



    Western blot analysis of immunised recombinant protein..

    This antibody has only been tested in WB against the recombinant fragment used as immunogen. We have no data on the detection of endogenous protein.

References

ab51236 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Answer

Thank you for contacting us. We are happy to help customers find the most suitable positive controls for their research purposes; however we are not specialized in this particular field and do not want to recommend a control that may be unsuitable. We encourage customers to consult the latest literature available through PubMed and other resources in order to find the most up-to-date information about their specific research interests. A preliminary literature search showed that human colon adenocarcinoma should be a suitable positive control. A Human colon adenocarcinoma cell line SW480 was also found to be positive for HOXA9. We do offer a SW480 lysate for WB that could then be used as positive control: ab3957 (SW480 (Human colon adenocarcinoma cell line) Whole Cell Lysate) Click here (or use the following: https://www.abcam.com/index.html?datasheet=3957). I also found that the following cells were found positive: 721_B cells and hep2G cells. I am sorry that I could not be more helpful, but I hope that the available literature in this area can provide some clarification. Please do not hesitate to contact us again with other needs or with any questions about our products.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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