Product nameAnti-HOXB13 antibody
See all HOXB13 primary antibodies
DescriptionRabbit polyclonal to HOXB13
Tested applicationsSuitable for: IHC-P, WB, ELISAmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Pufferfish, Zebrafish, Japanese ricefish
- HeLa cell lysate.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferPreservative: 0.025% Sodium azide
Constituent: 50% Glycerol
Our Abpromise guarantee covers the use of ab28575 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. PubMed: 24189220|
|WB||1/1000 - 1/5000. Detects a band of approximately 32 kDa (predicted molecular weight: 32 kDa).|
|ELISA||1/100 - 1/1000.|
FunctionSequence-specific transcription factor which is part of a developmental regulatory system that provides cells with specific positional identities on the anterior-posterior axis.
Sequence similaritiesBelongs to the Abd-B homeobox family.
Contains 1 homeobox DNA-binding domain.
- Information by UniProt
- Homeo box B13 antibody
- Homeobox B13 antibody
- Homeobox protein Hox B13 antibody
All lanes : Anti-HOXB13 antibody (ab28575) at 1/5000 dilution
Lane 1 : Hela cell lysate with no immunizing peptide
Lane 2 : Hela cell lysate with immunizing peptide
Lysates/proteins at 20 µg/ml per lane.
Predicted band size: 32 kDa
Observed band size: 32 kDa
Bands at ~40 kDa and ~50 kDa relate to to HOXC13 and HOXA13 which also react with this antibody and are blocked by its immunogen.
This product has been referenced in:
- Sui BQ et al. HOXB13 expression and promoter methylation as a candidate biomarker in gastric cancer. Oncol Lett 15:8833-8840 (2018). Read more (PubMed: 29928325) »
- Tatangelo F et al. Posterior HOX genes and HOTAIR expression in the proximal and distal colon cancer pathogenesis. J Transl Med 16:350 (2018). Read more (PubMed: 30541551) »