Overview

  • Product name

    Anti-HOXB13 antibody [EPR17371]
    See all HOXB13 primary antibodies
  • Description

    Rabbit monoclonal [EPR17371] to HOXB13
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human HOXB13 aa 250 to the C-terminus. The exact sequence is proprietary.
    Database link: Q92826

  • Positive control

    • WB: Human prostate cancer lysate; LNCaP and PC-3 cell lysates. IHC-P: Human carcinoma of prostate tissue.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab201682 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/5000. Detects a band of approximately 31 kDa (predicted molecular weight: 31 kDa).
IHC-P 1/3000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Target

  • Function

    Sequence-specific transcription factor which is part of a developmental regulatory system that provides cells with specific positional identities on the anterior-posterior axis.
  • Sequence similarities

    Belongs to the Abd-B homeobox family.
    Contains 1 homeobox DNA-binding domain.
  • Cellular localization

    Nucleus.
  • Information by UniProt
  • Database links

  • Alternative names

    • Homeo box B13 antibody
    • Homeobox B13 antibody
    • Homeobox protein Hox B13 antibody
    • Homeobox protein Hox-B13 antibody
    • Homeobox protein HoxB13 antibody
    • HOX B13 antibody
    • HOXB 13 antibody
    • HOXB13 antibody
    • HXB13_HUMAN antibody
    • PSGD antibody
    • PSGD protein antibody
    see all

Images

  • All lanes : Anti-HOXB13 antibody [EPR17371] (ab201682) at 1/5000 dilution

    Lane 1 : Human prostate cancer lysate
    Lane 2 : Human fetal brain lysate
    Lane 3 : Human fetal kidney lysate
    Lane 4 : Human fetal spleen lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

    Predicted band size: 31 kDa
    Observed band size: 31 kDa


    Exposure time: 1 minute


    Blocking/Dilution buffer: 5% NFDM/TBST.

    HOXB13 is predominantly expressed in the prostate.

  • All lanes : Anti-HOXB13 antibody [EPR17371] (ab201682) at 1/5000 dilution

    Lane 1 : LNCaP (Human prostate cancer cell line) cell lysate
    Lane 2 : PC-3 (Human prostate cancer cell line) cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 31 kDa
    Observed band size: 31 kDa


    Exposure time: 1 minute


    Blocking/Dilution buffer: 5% NFDM/TBST.

    HOXB13 is predominantly expressed in the prostate.

  • Immunohistochemical analysis of paraffin-embedded Human carcinoma of prostate tissue labeling HOXB13 with ab201682 at 1/3000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) secondary antibody at 1/500 dilution. Nuclear staining on Human carcinoma of prostate tissue is observed. Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemical analysis of paraffin-embedded Human transitional cell carcinoma of bladder tissue labeling HOXB13 with ab201682 at 1/3000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) secondary antibody at 1/500 dilution. Human transitional cell carcinoma of bladder represents a HOXB13 negative tissue. Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemical analysis of paraffin-embedded Human cerebral cortex tissue labeling HOXB13 with ab201682 at 1/3000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) secondary antibody at 1/500 dilution. Human cerebral cortex represents a HOXB13 negative tissue. Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

References

ab201682 has not yet been referenced specifically in any publications.

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