Overview

  • Product name

  • Description

    Rabbit polyclonal to Hoxb3
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, WB, ELISAmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Dog
  • Immunogen

    Synthetic peptide within residues: GPSKSGPPKC GPGTNSTLTK QIFPWMKESR QTSKLKNNSP GTAEGCGGGG , corresponding to N terminal amino acids 108-157 of Human Hoxb3 (NP_002137)

  • Positive control

    • HepG2 cell lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab83404 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use at an assay dependent concentration. PubMed: 25745994
WB Use a concentration of 1 µg/ml. Detects a band of approximately 44 kDa (predicted molecular weight: 44 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ELISA Use at an assay dependent concentration.

ELISA titre using peptide based assay: 1/312500.

Target

  • Function

    Sequence-specific transcription factor which is part of a developmental regulatory system that provides cells with specific positional identities on the anterior-posterior axis.
  • Sequence similarities

    Belongs to the Antp homeobox family.
    Contains 1 homeobox DNA-binding domain.
  • Developmental stage

    Expressed in whole embryos and fetuses at 5-9 weeks from conception.
  • Cellular localization

    Nucleus.
  • Information by UniProt
  • Database links

  • Alternative names

    • Homeo box 2G antibody
    • Homeo box B3 antibody
    • Homeobox B3 antibody
    • Homeobox protein Hox-2.7 antibody
    • Homeobox protein Hox-2G antibody
    • Homeobox protein Hox-B3 antibody
    • Homeobox protein HoxB3 antibody
    • Hox 2.7 antibody
    • HOX 2G antibody
    • HOX2 antibody
    • Hox2.7 antibody
    • HOX2G antibody
    • HOXB3 antibody
    • HXB3_HUMAN antibody
    see all

Images

  • Anti-Hoxb3 antibody (ab83404) at 1 µg/ml (in 5% skim milk / PBS buffer) + HepG2 cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size: 44 kDa
    Observed band size: 44 kDa



    Gel concentration 12%

References

This product has been referenced in:

  • Han C  et al. Knockdown of lncRNA-UCA1 inhibits the proliferation and migration of melanoma cells through modulating the miR-28-5p/HOXB3 axis. Exp Ther Med 17:4294-4302 (2019). Read more (PubMed: 30988802) »
  • Jiang Y  et al. Repression of Hox genes by LMP1 in nasopharyngeal carcinoma and modulation of glycolytic pathway genes by HoxC8. Oncogene 34:6079-91 (2015). IHC-P ; Human . Read more (PubMed: 25745994) »
See all 2 Publications for this product

Customer reviews and Q&As

1-2 of 2 Abreviews or Q&A

Question
Answer

I have processed your request to receive a refund for ab83404 which did not work in western blot. Your credit note number is ******.

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Answer

Thank you for your reply and for providing the order details. ab843404 is covered under our Abpromsie and so is guaranteed to work in western blotting on human samples. As it is clearly not working as promised, then I can either send you a replacement antibody or process a refund. We do have another Hox3b antibody in our catalogue, ab82945, which will work on human samples in western blot. I look forward to your reply.

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