Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR1917] to HOXB4
- Suitable for: ICC/IF, WB
- Reacts with: Mouse, Rat, Human
Product nameAnti-HOXB4 antibody [EPR1917]
See all HOXB4 primary antibodies
DescriptionRabbit monoclonal [EPR1917] to HOXB4
Tested applicationsSuitable for: ICC/IF, WBmore details
Unsuitable for: Flow Cyt or IHC-P
Species reactivityReacts with: Mouse, Rat, Human
Synthetic peptide within Human HOXB4 aa 1-100. The exact sequence is proprietary.
- K562, Jurkat, RAW 264.7, and PC-12 cell lysates; NCCIT cells.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Dissociation constant (KD)KD = 1.49 x 10 -11 M Learn more about KD
Storage bufferpH: 7.2
Preservative: 0.05% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant
Concentration information loading...
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab133521 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||1/100 - 1/200.|
|WB||1/1000 - 1/10000. Predicted molecular weight: 34 kDa.|
FunctionSequence-specific transcription factor which is part of a developmental regulatory system that provides cells with specific positional identities on the anterior-posterior axis.
Sequence similaritiesBelongs to the Antp homeobox family. Deformed subfamily.
Contains 1 homeobox DNA-binding domain.
Developmental stageExpressed in whole embryos and fetuses at 5-9 weeks from conception.
- Information by UniProt
- Homeo box 2F antibody
- Homeo box B4 antibody
- Homeobox 2F antibody
All lanes : Anti-HOXB4 antibody [EPR1917] (ab133521) at 1/1000 dilution
Lane 1 : K562 cell lysate
Lane 2 : Jurkat cell lysate
Lane 3 : RAW 264.7 cell lysate
Lane 4 : PC-12 cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 34 kDa
Immunofluorescence analysis of NCCIT (Human pluripotent embryonic carcinoma cell line) cells labeling HOXB4 with ab133521 at 1/100 dilution.
Equilibrium disassociation constant (KD)
Learn more about KD
Click here to learn more about KD
ab133521 has been referenced in 2 publications.
- Lim GS et al. Microhexagon gradient array directs spatial diversification of spinal motor neurons. Theranostics 9:311-323 (2019). PubMed: 30809276
- Hor JH et al. Cell cycle inhibitors protect motor neurons in an organoid model of Spinal Muscular Atrophy. Cell Death Dis 9:1100 (2018). PubMed: 30368521