Overview

  • Product name

    Anti-HOXB4 antibody [EPR1917]
    See all HOXB4 primary antibodies
  • Description

    Rabbit monoclonal [EPR1917] to HOXB4
  • Host species

    Rabbit
  • Tested applications

    Suitable for: ICC/IF, WB, IPmore details
    Unsuitable for: Flow Cyt or IHC-P
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human HOXB4 aa 1-100. The exact sequence is proprietary.

  • Positive control

    • K562, Jurkat, RAW 264.7, and PC-12 cell lysates; NCCIT cells.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab133521 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/100 - 1/200.
WB 1/1000 - 1/10000. Predicted molecular weight: 34 kDa.
IP 1/10 - 1/100.
  • Application notes
    Is unsuitable for Flow Cyt or IHC-P.
  • Target

    • Function

      Sequence-specific transcription factor which is part of a developmental regulatory system that provides cells with specific positional identities on the anterior-posterior axis.
    • Sequence similarities

      Belongs to the Antp homeobox family. Deformed subfamily.
      Contains 1 homeobox DNA-binding domain.
    • Developmental stage

      Expressed in whole embryos and fetuses at 5-9 weeks from conception.
    • Cellular localization

      Nucleus.
    • Information by UniProt
    • Database links

    • Alternative names

      • Homeo box 2F antibody
      • Homeo box B4 antibody
      • Homeobox 2F antibody
      • Homeobox B4 antibody
      • Homeobox protein Hox B4 antibody
      • Homeobox protein Hox-2.6 antibody
      • Homeobox protein Hox-2F antibody
      • Homeobox protein Hox-B4 antibody
      • Homeobox protein HoxB4 antibody
      • HOX 2 antibody
      • Hox 2.6 antibody
      • Hox 2F antibody
      • HOX B4 antibody
      • HOX2 antibody
      • Hox2.6 antibody
      • Hox2F antibody
      • HOXB 4 antibody
      • hoxb4 antibody
      • HXB4_HUMAN antibody
      see all

    Images

    • All lanes : Anti-HOXB4 antibody [EPR1917] (ab133521) at 1/1000 dilution

      Lane 1 : K562 cell lysate
      Lane 2 : Jurkat cell lysate
      Lane 3 : RAW 264.7 cell lysate
      Lane 4 : PC-12 cell lysate

      Lysates/proteins at 10 µg per lane.

      Predicted band size: 34 kDa

    • Immunofluorescence analysis of NCCIT (Human pluripotent embryonic carcinoma cell line) cells labeling HOXB4 with ab133521 at 1/100 dilution.

    • Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD

    References

    This product has been referenced in:

    • Lim GS  et al. Microhexagon gradient array directs spatial diversification of spinal motor neurons. Theranostics 9:311-323 (2019). Read more (PubMed: 30809276) »
    • Hor JH  et al. Cell cycle inhibitors protect motor neurons in an organoid model of Spinal Muscular Atrophy. Cell Death Dis 9:1100 (2018). Read more (PubMed: 30368521) »
    See all 2 Publications for this product

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab133521.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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