Product nameAnti-HOXB4 antibody [EPR1917]
See all HOXB4 primary antibodies
DescriptionRabbit monoclonal [EPR1917] to HOXB4
Tested applicationsSuitable for: ICC/IF, WB, IPmore details
Unsuitable for: Flow Cyt or IHC-P
Species reactivityReacts with: Mouse, Rat, Human
Synthetic peptide within Human HOXB4 aa 1-100. The exact sequence is proprietary.
- K562, Jurkat, RAW 264.7, and PC-12 cell lysates; NCCIT cells.
Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Dissociation constant (KD)KD = 1.49 x 10 -11 M Learn more about KD
Storage bufferpH: 7.40
Preservative: 0.05% Sodium azide
Constituents: 40% Glycerol, 9.85% Tris glycine, 50% Tissue culture supernatant
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab133521 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||1/100 - 1/200.|
|WB||1/1000 - 1/10000. Predicted molecular weight: 34 kDa.|
|IP||1/10 - 1/100.|
FunctionSequence-specific transcription factor which is part of a developmental regulatory system that provides cells with specific positional identities on the anterior-posterior axis.
Sequence similaritiesBelongs to the Antp homeobox family. Deformed subfamily.
Contains 1 homeobox DNA-binding domain.
Developmental stageExpressed in whole embryos and fetuses at 5-9 weeks from conception.
- Information by UniProt
- Homeo box 2F antibody
- Homeo box B4 antibody
- Homeobox 2F antibody
All lanes : Anti-HOXB4 antibody [EPR1917] (ab133521) at 1/1000 dilution
Lane 1 : K562 cell lysate
Lane 2 : Jurkat cell lysate
Lane 3 : RAW 264.7 cell lysate
Lane 4 : PC-12 cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 34 kDa
Immunofluorescence analysis of NCCIT (Human pluripotent embryonic carcinoma cell line) cells labeling HOXB4 with ab133521 at 1/100 dilution.
Equilibrium disassociation constant (KD)
Learn more about KD
Click here to learn more about KD
This product has been referenced in:
- Lim GS et al. Microhexagon gradient array directs spatial diversification of spinal motor neurons. Theranostics 9:311-323 (2019). Read more (PubMed: 30809276) »
- Hor JH et al. Cell cycle inhibitors protect motor neurons in an organoid model of Spinal Muscular Atrophy. Cell Death Dis 9:1100 (2018). Read more (PubMed: 30368521) »