Key features and details
- Rabbit polyclonal to HOXB5
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
Product nameAnti-HOXB5 antibody
See all HOXB5 primary antibodies
DescriptionRabbit polyclonal to HOXB5
Tested applicationsSuitable for: WB, IPmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat
Recombinant fragment within Human HOXB5 (internal sequence). The exact sequence is proprietary.
Database link: P09067
- WB: HepG2 whole cell and nuclear extracts. IP: PC-3 nuclear extracts.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine)
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab229345 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/3000. Predicted molecular weight: 29 kDa.|
|IP||1/100 - 1/500.|
FunctionSequence-specific transcription factor which is part of a developmental regulatory system that provides cells with specific positional identities on the anterior-posterior axis.
Tissue specificitySpinal cord.
Sequence similaritiesBelongs to the Antp homeobox family.
Contains 1 homeobox DNA-binding domain.
- Information by UniProt
- HGNC:5116 antibody
- HHO.C10 antibody
- Homeo box 2A antibody
All lanes : Anti-HOXB5 antibody (ab229345) at 1/500 dilution
Lane 1 : HepG2 (human liver hepatocellular carcinoma cell line) whole cell extract
Lane 2 : HepG2 nuclear extract
Lysates/proteins at 30 µg per lane.
Predicted band size: 29 kDa
12 % SDS-PAGE gel.
HOXB5 was immunoprecipitated from PC-3 (human prostate adenocarcinoma cell line) nuclear extract with 5 µg ab229345. Western blot was performed from the immunoprecipitate using ab229345. Anti-Rabbit IgG was used as a secondary reagent.
Lane 1: Control IgG IP in PC-3 nuclear extract.
Lane 2: ab229345 IP in PC-3 nuclear extract.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab229345 has not yet been referenced specifically in any publications.