Overview

  • Product name

  • Description

    Rabbit polyclonal to HOXB5
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IPmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Recombinant fragment within Human HOXB5 (internal sequence). The exact sequence is proprietary.
    Database link: P09067

  • Positive control

    • WB: HepG2 whole cell and nuclear extracts. IP: PC-3 nuclear extracts.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.00
    Preservative: 0.025% Proclin
    Constituents: PBS, 20% Glycerol
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab229345 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 29 kDa.
IP 1/100 - 1/500.

Target

  • Function

    Sequence-specific transcription factor which is part of a developmental regulatory system that provides cells with specific positional identities on the anterior-posterior axis.
  • Tissue specificity

    Spinal cord.
  • Sequence similarities

    Belongs to the Antp homeobox family.
    Contains 1 homeobox DNA-binding domain.
  • Developmental stage

    Embryo.
  • Cellular localization

    Nucleus.
  • Information by UniProt
  • Database links

  • Alternative names

    • HGNC:5116 antibody
    • HHO.C10 antibody
    • Homeo box 2A antibody
    • Homeo box B5 antibody
    • Homeobox 2A antibody
    • Homeobox B5 antibody
    • Homeobox protein HHO.C10 antibody
    • Homeobox protein Hox B5 antibody
    • Homeobox protein Hox-2A antibody
    • Homeobox protein Hox-B5 antibody
    • Homeobox protein HoxB5 antibody
    • Homeobox protein Hu-1 antibody
    • Homeobox2A antibody
    • HomeoboxB5 antibody
    • HOX 2 antibody
    • Hox 2.1 antibody
    • HOX 2A antibody
    • HOX B5 antibody
    • HOX2 antibody
    • Hox2.1 antibody
    • Hox2.1, mouse, homolog of antibody
    • HOX2A antibody
    • HOXB 5 antibody
    • Hoxb5 antibody
    • HU 1 antibody
    • HU1 antibody
    • HXB5_HUMAN antibody
    see all

Images

  • All lanes : Anti-HOXB5 antibody (ab229345) at 1/500 dilution

    Lane 1 : HepG2 (human liver hepatocellular carcinoma cell line) whole cell extract
    Lane 2 : HepG2 nuclear extract

    Lysates/proteins at 30 µg per lane.

    Predicted band size: 29 kDa



    12 % SDS-PAGE gel.

  • HOXB5 was immunoprecipitated from PC-3 (human prostate adenocarcinoma cell line) nuclear extract with 5 µg ab229345. Western blot was performed from the immunoprecipitate using ab229345. Anti-Rabbit IgG was used as a secondary reagent.

    Lane 1: Control IgG IP in PC-3 nuclear extract.

    Lane 2: ab229345 IP in PC-3 nuclear extract.

References

ab229345 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab229345.
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