Overview

  • Product name

  • Description

    Rabbit polyclonal to HOXB9
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, ELISA, IHC-Pmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Rabbit, Horse, Cow, Cat, Dog, Pig
  • Immunogen

    A region within synthetic peptide: SLPSVYHPYI QPQGVPPAES RYLRTWLEPA PRGEAAPGQG QAAVKAEPLL, corresponding to amino acids 73-122 of Human HOXB9

  • Positive control

    • HepG2 whole cell lysate (ab7900), human heart tissue and human lung tissue

Properties

Applications

Our Abpromise guarantee covers the use of ab66765 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.5 - 1.25 µg/ml. Detects a band of approximately 28, 32, 85 kDa (predicted molecular weight: 28 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ELISA Use at an assay dependent concentration.

ELISA titre using peptide based assay 1:312500.

IHC-P Use a concentration of 4 - 8 µg/ml.

Target

  • Function

    Sequence-specific transcription factor which is part of a developmental regulatory system that provides cells with specific positional identities on the anterior-posterior axis.
  • Sequence similarities

    Belongs to the Abd-B homeobox family.
    Contains 1 homeobox DNA-binding domain.
  • Developmental stage

    Expressed in whole embryos and fetuses at 5-9 weeks from conception.
  • Cellular localization

    Nucleus.
  • Information by UniProt
  • Database links

  • Alternative names

    • Homeo box 2E antibody
    • Homeo box B9 antibody
    • Homeobox B9 antibody
    • Homeobox protein Hox B9 antibody
    • Homeobox protein Hox-2.5 antibody
    • Homeobox protein Hox-2E antibody
    • Homeobox protein Hox-B9 antibody
    • Hox 2.5 antibody
    • Hox 2E antibody
    • HOX2 antibody
    • HOX2E antibody
    • Hoxb9 antibody
    • HXB9_HUMAN antibody
    • OTTHUMP00000216946 antibody
    • OTTHUMP00000243325 antibody
    see all

Images

  • Anti-HOXB9 antibody (ab66765) at 1.25 µg/ml + HepG2 cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size: 28 kDa
    Observed band size: 28 kDa
    Additional bands at: 30 kDa, 85 kDa. We are unsure as to the identity of these extra bands.



    There are also three other bands < 23kDa. We are unsure as to the identity of these extra bands.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human lung tissue labelling HOXB9 with ab66765 at 4-8µg/ml. Arrows indicate positively labelled alveolar cells. Magnification: 400X.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human heart tissue labelling HOXB9 with ab66765 at 4-8µg/ml. Arrows indicate positively labelled myocardial cells. Magnification: 400X.

References

This product has been referenced in:

  • Dell'Anno MT  et al. Human neuroepithelial stem cell regional specificity enables spinal cord repair through a relay circuit. Nat Commun 9:3419 (2018). Read more (PubMed: 30143638) »
  • Wu SY  et al. A miR-192-EGR1-HOXB9 regulatory network controls the angiogenic switch in cancer. Nat Commun 7:11169 (2016). WB, IHC-P ; Human . Read more (PubMed: 27041221) »
See all 3 Publications for this product

Customer reviews and Q&As

1-4 of 4 Abreviews or Q&A

Answer

Thank you for your reply and for your interest in ab66765. I will have the blocking peptide added to the catalog, and I'll let you know the catalog number once this is completed. Since ab66765 has not been tested in bovine or murine samples, if you would like to test it in one of these species, you are eligible for our testing discount program. For this program, you would purchase the antibody at full price, submit an Abreview with your data, and in exchange we will give you one free primary antibody on a future purchase (must be requested within 4 months of ab66765 purchase). If you would like to participate in this program, let me know and I will create a discount code for you that can be used to redeem your free antibody. I look forward to hearing from you. Please let me know if you have any further questions.

Read More

Answer

Thank you for contacting us with your questions. We have not tested this antibody yet in bovine or murine tissue, however the immunogen is 100% homologous to the bovine sequence so it will most likely work. The immunogen is only 78% homologous to mouse so it is less likely to work in this species. Since we have not directly tested these species, we are unable to guarantee that ab66765 will work, however we do have a testing discount program if you would be interested in testing these antibodies in bovine or mouse samples. The blocking peptide is not currently listed in our catalog, but if you are interested in ordering it I will have it added to the catalog. The pre-immune serum is not available, however we do have non-immune rabbit serum ab7487 if you would like to use that as a control- https://www.abcam.com/Rabbit-Serum-sterile-ab7487.html I hope this information is useful, but please let me know if you have further questions and I'll be happy to help.

Read More

Question
Answer

Thank you for contacting us and please accept all our apologies for this error and delay. Indeed, we had a confusion in our notes in regards to the reconstitution of this antibody. I can confirm now, that the vial you have received has been reconstituted in the appropriate buffer and to 50ul. The vial you have received corresponds therefore to a final concentration of 1mg/ml. I hope this information is helpful to you. Please accept once again our apologies for this confusion. Please do not hesitate to contact us if you need any more advice or information.

Read More
Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (Spinal cord)
Specification
Spinal cord
Fixative
Paraformaldehyde
Permeabilization
Yes - 0.1% Triton
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 25°C

Abcam user community

Verified customer

Submitted Oct 21 2009

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Sign up