Product nameAnti-HOXB9 antibody
See all HOXB9 primary antibodies
DescriptionRabbit polyclonal to HOXB9
Tested applicationsSuitable for: WB, ELISA, IHC-Pmore details
Species reactivityReacts with: Human
Predicted to work with: Rabbit, Horse, Cow, Cat, Dog, Pig
- HepG2 whole cell lysate (ab7900), human heart tissue and human lung tissue
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage bufferPreservative: 0.09% Sodium azide
Constituents: 2% Sucrose, PBS
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab66765 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 0.5 - 1.25 µg/ml. Detects a band of approximately 28, 32, 85 kDa (predicted molecular weight: 28 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.|
|ELISA||Use at an assay dependent concentration.
ELISA titre using peptide based assay 1:312500.
|IHC-P||Use a concentration of 4 - 8 µg/ml.|
FunctionSequence-specific transcription factor which is part of a developmental regulatory system that provides cells with specific positional identities on the anterior-posterior axis.
Sequence similaritiesBelongs to the Abd-B homeobox family.
Contains 1 homeobox DNA-binding domain.
Developmental stageExpressed in whole embryos and fetuses at 5-9 weeks from conception.
- Information by UniProt
- Homeo box 2E antibody
- Homeo box B9 antibody
- Homeobox B9 antibody
Anti-HOXB9 antibody (ab66765) at 1.25 µg/ml + HepG2 cell lysate at 10 µg
HRP conjugated anti-Rabbit IgG at 1/50000 dilution
Predicted band size: 28 kDa
Observed band size: 28 kDa
Additional bands at: 30 kDa, 85 kDa. We are unsure as to the identity of these extra bands.
There are also three other bands < 23kDa. We are unsure as to the identity of these extra bands.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human lung tissue labelling HOXB9 with ab66765 at 4-8µg/ml. Arrows indicate positively labelled alveolar cells. Magnification: 400X.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human heart tissue labelling HOXB9 with ab66765 at 4-8µg/ml. Arrows indicate positively labelled myocardial cells. Magnification: 400X.
This product has been referenced in:
- Dell'Anno MT et al. Human neuroepithelial stem cell regional specificity enables spinal cord repair through a relay circuit. Nat Commun 9:3419 (2018). Read more (PubMed: 30143638) »
- Wu SY et al. A miR-192-EGR1-HOXB9 regulatory network controls the angiogenic switch in cancer. Nat Commun 7:11169 (2016). WB, IHC-P ; Human . Read more (PubMed: 27041221) »