Overview

  • Product name

    Anti-HOXB9 antibody [EPR6950]
    See all HOXB9 primary antibodies
  • Description

    Rabbit monoclonal [EPR6950] to HOXB9
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IP, ICCmore details
    Unsuitable for: Flow Cyt or IHC-P
  • Species reactivity

    Reacts with: Human
  • Immunogen

    A synthetic peptide corresponding to residues in Human HOXB9 (UniProt ID: P17482)

  • Positive control

    • WB: MCF7 cell lysate
  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab133701 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 28 kDa (predicted molecular weight: 28 kDa).
IP 1/10 - 1/100.
ICC 1/50 - 1/100.
  • Application notes
    Is unsuitable for Flow Cyt or IHC-P.
  • Target

    • Function

      Sequence-specific transcription factor which is part of a developmental regulatory system that provides cells with specific positional identities on the anterior-posterior axis.
    • Sequence similarities

      Belongs to the Abd-B homeobox family.
      Contains 1 homeobox DNA-binding domain.
    • Developmental stage

      Expressed in whole embryos and fetuses at 5-9 weeks from conception.
    • Cellular localization

      Nucleus.
    • Information by UniProt
    • Database links

    • Alternative names

      • Homeo box 2E antibody
      • Homeo box B9 antibody
      • Homeobox B9 antibody
      • Homeobox protein Hox B9 antibody
      • Homeobox protein Hox-2.5 antibody
      • Homeobox protein Hox-2E antibody
      • Homeobox protein Hox-B9 antibody
      • Hox 2.5 antibody
      • Hox 2E antibody
      • HOX2 antibody
      • HOX2E antibody
      • Hoxb9 antibody
      • HXB9_HUMAN antibody
      • OTTHUMP00000216946 antibody
      • OTTHUMP00000243325 antibody
      see all

    Images

    • Anti-HOXB9 antibody [EPR6950] (ab133701) at 1/1000 dilution + MCF7 cell lysate at 10 µg

      Secondary
      HRP labelled Goat anti Rabbit IgG at 1/2000 dilution

      Predicted band size: 28 kDa
      Observed band size: 28 kDa

    • Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD

    References

    This product has been referenced in:

    • Darda L  et al. The role of HOXB9 and miR-196a in head and neck squamous cell carcinoma. PLoS One 10:e0122285 (2015). WB . Read more (PubMed: 25860510) »
    • Clarke-Harris R  et al. PGC1a promoter methylation in blood at 5-7 years predicts adiposity from 9 to 14 years (EarlyBird 50). Diabetes 63:2528-37 (2014). Human . Read more (PubMed: 24622795) »
    See all 2 Publications for this product

    Customer reviews and Q&As

    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cultured Cells (Differentiated human ES cells)
    Permeabilization
    Yes - PBS/Triton X-100
    Specification
    Differentiated human ES cells
    Blocking step
    (agent) for 3 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 21°C
    Fixative
    Paraformaldehyde

    Abcam user community

    Verified customer

    Submitted Apr 17 2019

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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