Overview

Properties

Applications

Our Abpromise guarantee covers the use of ab153904 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 38 kDa.
IHC-P 1/100 - 1/1000.
ICC/IF 1/100 - 1/1000.

Target

  • Function

    Sequence-specific transcription factor which is part of a developmental regulatory system that provides cells with specific positional identities on the anterior-posterior axis.
  • Sequence similarities

    Belongs to the Abd-B homeobox family.
    Contains 1 homeobox DNA-binding domain.
  • Cellular localization

    Nucleus.
  • Information by UniProt
  • Database links

  • Alternative names

    • Hmeobox protein Hox C10 antibody
    • Homeobox Protein Hox C10 antibody
    • Homeobox protein Hox-3I antibody
    • Homeobox protein Hox-C10 antibody
    • Homeoprotein C10 antibody
    • Hox-3.6 antibody
    • HOX3I antibody
    • HOXC10 antibody
    • HXC10_HUMAN antibody
    • MGC5259 antibody
    see all

Images

  • Immunofluorescent analysis of paraformaldehyde-fixed HeLa cells labeling HOXC10 with ab153904 at 1/2000 dilution. Alpha tubulin filaments were labeled red.

  • All lanes : Anti-HOXC10 antibody (ab153904) at 1/1000 dilution

    Lane 1 : HeLa whole cell lysate
    Lane 2 : HepG2 whole cell lysate

    Lysates/proteins at 30 µg per lane.

    Predicted band size: 38 kDa



    10% SDS PAGE
  • Immunohistochemical analysis of paraffin-embedded HeLa xenograft tissue labeling HOXC10 with ab153904 at 1/500 dilution.

  • Anti-HOXC10 antibody (ab153904) at 1/1000 dilution + Mouse muscle lysate at 50 µg

    Predicted band size: 38 kDa



    10% SDS PAGE

References

This product has been referenced in:

  • Miwa T  et al. Homeobox C10 Influences on the Malignant Phenotype of Gastric Cancer Cell Lines and its Elevated Expression Positively Correlates with Recurrence and Poor Survival. Ann Surg Oncol N/A:N/A (2019). Read more (PubMed: 30673899) »
  • Jain D  et al. Regulatory integration of Hox factor activity with T-box factors in limb development. Development 145:N/A (2018). Read more (PubMed: 29490982) »
See all 4 Publications for this product

Customer reviews and Q&As

Application
Immunocytochemistry/ Immunofluorescence
Blocking step
Serum as blocking agent for 16 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 4°C
Sample
Human Cell (Differentiated human embryonic stem cells)
Specification
Differentiated human embryonic stem cells
Permeabilization
Yes - PBST
Fixative
Paraformaldehyde

Abcam user community

Verified customer

Submitted Aug 04 2014

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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