Overview

  • Product name

  • Description

    Rabbit polyclonal to HoxC6

    This product is a fast track antibody. It has been affinity purified and shows high titre values against the immunizing peptide by ELISA. Read the terms of use »

  • Host species

    Rabbit
  • Species reactivity

    Reacts with: Mouse
    Predicted to work with: Rat, Human
  • Immunogen

    Synthetic peptide corresponding to Human HoxC6 aa 100-200 conjugated to keyhole limpet haemocyanin.
    (Peptide available as ab41586)

  • Positive control

    • Recombinant Human HoxC6 protein (ab116795) can be used as a positive control in WB.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.02% Sodium Azide
    Constituents: 1% BSA, PBS, pH 7.4
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Fast track antibodies constitute a diverse group of products that have been released to accelerate your research, but are not yet fully characterized. They have all been affinity purified and show high titre values against the immunizing peptide (by ELISA). Fast track terms of use

Application Abreviews Notes
ICC/IF Use at an assay dependent concentration. PubMed: 23946489
IHC-P 1/50. Some customers have used this antibody successfully in IHC-P; however this is not a tested application for this antibody.
WB Use at an assay dependent concentration. Predicted molecular weight: 26 kDa.

Target

  • Function

    Sequence-specific transcription factor which is part of a developmental regulatory system that provides cells with specific positional identities on the anterior-posterior axis.
  • Sequence similarities

    Belongs to the Antp homeobox family.
    Contains 1 homeobox DNA-binding domain.
  • Cellular localization

    Nucleus.
  • Information by UniProt
  • Database links

  • Alternative names

    • CP25 antibody
    • HHO.C8 antibody
    • Homeo box 3C antibody
    • Homeo box C6 antibody
    • Homeo box C8 protein antibody
    • Homeobox C6 antibody
    • Homeobox protein CP25 antibody
    • Homeobox protein HHO.C8 antibody
    • Homeobox protein Hox-3C antibody
    • Homeobox protein Hox-C6 antibody
    • HOX3 antibody
    • HOX3C antibody
    • Hoxc6 antibody
    • HXC6_HUMAN antibody
    see all

Images

This Fast-Track antibody is not yet fully characterised. These images represent inconclusive preliminary data.

  • All lanes : Anti-HoxC6 antibody (ab41587)

    Lane 1 : Human ovary tissue lysate
    Lane 2 : Human ovary tumor tissue lysate
    Lane 3 : SK-N-BE cell lysate
    Lane 4 : PC3 cell lysate
    Lane 5 : MCF7 cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP) at 1/50000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 26 kDa
    Observed band size: 25 kDa
    why is the actual band size different from the predicted?


    Exposure time: 8 minutes

References

This product has been referenced in:

  • Zhang F  et al. HOXC6 gene silencing inhibits epithelial-mesenchymal transition and cell viability through the TGF-ß/smad signaling pathway in cervical carcinoma cells. Cancer Cell Int 18:204 (2018). Read more (PubMed: 30559605) »
  • Chang SL  et al. HOXC6 Overexpression Is Associated With Ki-67 Expression and Poor Survival in NPC Patients. J Cancer 8:1647-1654 (2017). Read more (PubMed: 28775784) »
See all 7 Publications for this product

Customer reviews and Q&As

1-3 of 3 Abreviews or Q&A

Application
Immunocytochemistry
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 2% · Temperature: 21°C
Sample
Mouse Cultured Cells (lateral motor column)
Specification
lateral motor column
Permeabilization
No
Fixative
Paraformaldehyde

Mr. Dan Stummer

Verified customer

Submitted Apr 15 2014

Answer

Thank you for contacting Abcam.

Unfortunately we do not have any more of lot 414179 in out stock, and because it is a Fast Track antibody, we cannot guarantee it to work in any application other that an ELISA with the immunizing peptide.

If you can send me a copy of the protocol that you have been using, I will be more than happy to see if there is any suggestions I can make.

If there is anything else I can help you with, please let me know.

Read More
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (Prostate)
Specification
Prostate
Fixative
Formaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citrate
Permeabilization
No
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 4°C

Abcam user community

Verified customer

Submitted Apr 01 2008

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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