Overview

  • Product name

    Anti-HoxC6 antibody [EPR7922(2)]
    See all HoxC6 primary antibodies
  • Description

    Rabbit monoclonal [EPR7922(2)] to HoxC6
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, Flow Cytmore details
    Unsuitable for: ICC,IHC-P or IP
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human HoxC6 aa 1-100. The exact sequence is proprietary.

  • Positive control

    • HUVEC, PC3, MCF7 and Jurkat whole cell lysate (ab7899); MCF7 cells.
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab151575 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 26 kDa.
Flow Cyt 1/100 - 1/500.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 

  • Application notes
    Is unsuitable for ICC,IHC-P or IP.
  • Target

    • Function

      Sequence-specific transcription factor which is part of a developmental regulatory system that provides cells with specific positional identities on the anterior-posterior axis.
    • Sequence similarities

      Belongs to the Antp homeobox family.
      Contains 1 homeobox DNA-binding domain.
    • Cellular localization

      Nucleus.
    • Information by UniProt
    • Database links

    • Alternative names

      • CP25 antibody
      • HHO.C8 antibody
      • Homeo box 3C antibody
      • Homeo box C6 antibody
      • Homeo box C8 protein antibody
      • Homeobox C6 antibody
      • Homeobox protein CP25 antibody
      • Homeobox protein HHO.C8 antibody
      • Homeobox protein Hox-3C antibody
      • Homeobox protein Hox-C6 antibody
      • HOX3 antibody
      • HOX3C antibody
      • Hoxc6 antibody
      • HXC6_HUMAN antibody
      see all

    Images

    • All lanes : Anti-HoxC6 antibody [EPR7922(2)] (ab151575) at 1/1000 dilution

      Lane 1 : HUVEC cell lysate
      Lane 2 : PC3 cell lysate
      Lane 3 : MCF7 cell lysate
      Lane 4 : Jurkat cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat anti-rabbit HRP at 1/2000 dilution

      Predicted band size: 26 kDa

    • Flow cytometric analysis of permeabilized MCF7 cells labeling HoxC6 with ab151575 at 1/100 dilution (red), compared to a Rabbit IgG negative control antibody (green).

    References

    This product has been referenced in:

    • Yan TF  et al. Knockdown of HOXC6 inhibits glioma cell proliferation and induces cell cycle arrest by targeting WIF-1 in vitro and vivo. Pathol Res Pract 214:1818-1824 (2018). Read more (PubMed: 30228024) »
    • Zhang F  et al. HOXC6 gene silencing inhibits epithelial-mesenchymal transition and cell viability through the TGF-ß/smad signaling pathway in cervical carcinoma cells. Cancer Cell Int 18:204 (2018). Read more (PubMed: 30559605) »
    See all 3 Publications for this product

    Customer reviews and Q&As

    Answer

    We currently have over forty products against these HOX targets. These product will be specific to the HOX protein which they are raised against, whether A1, A2, A3 etc. We currently do not carry an anti-HOX antibody which will recognize a number of HOX proteins. While we do not test for and cannot guarantee efficacy of our antibodies in EMSA super shift, I have reviewed the catalog for products which would be most suitable for this assay. Generally we would want to look for monoclonal antibodies which have been show to recognize the protein in a native form. For targets which do not have monoclonal antibodies available, I have chosen the best available alternative for you. This list is included in the spreadsheet which I have attached to this email.

    Read More

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