Key features and details
- Goat polyclonal to HOXD10
- Suitable for: IHC-P, WB
- Reacts with: Human
- Isotype: IgG
Product nameAnti-HOXD10 antibody
See all HOXD10 primary antibodies
DescriptionGoat polyclonal to HOXD10
Tested applicationsSuitable for: IHC-P, WBmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Horse, Cow, Xenopus laevis, Chimpanzee
- Human Kidney lysate.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
Storage bufferpH: 7.30
Preservative: 0.02% Sodium azide
Constituents: Tris buffered saline, 0.5% BSA
Concentration information loading...
PurityImmunogen affinity purified
Purification notesPurified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Our Abpromise guarantee covers the use of ab85698 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||Use a concentration of 3 - 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|WB||Use a concentration of 0.2 - 0.6 µg/ml. Predicted molecular weight: 38 kDa.
1 hour primary incubation is recommended for this product.
FunctionSequence-specific transcription factor which is part of a developmental regulatory system that provides cells with specific positional identities on the anterior-posterior axis.
Tissue specificityStrongly expressed in the adult male and female urogenital tracts.
Involvement in diseaseDefects in HOXD10 are a cause of congenital vertical talus (CVT) [MIM:192950]; also known as rocker-bottom foot deformity or congenital convex pes valgus. CVT is a dislocation of the talonavicular joint, with rigid dorsal dislocation of the navicular over the neck of the talus. This condition is usually associated with multiple other congenital deformities and only rarely is an isolated deformity.
Sequence similaritiesBelongs to the Abd-B homeobox family.
Contains 1 homeobox DNA-binding domain.
Developmental stageExpressed in the developing limb buds.
- Information by UniProt
- AI385591 antibody
- AI874987 antibody
- Homeo box 4D antibody
ab85698 (3.8µg/ml) staining of paraffin embedded Human Kidney following steamed antigen retrieval with citrate buffer pH 6 and AP-staining shows nuclear staining in some epithelial cells of PCT.
Anti-HOXD10 antibody (ab85698) at 0.2 µg/ml + Human Kidney lysate at 35 µg
Predicted band size: 38 kDa
Observed band size: 38 kDa
Additional bands at: 26 kDa, 90 kDa. We are unsure as to the identity of these extra bands.
Additional bands of unknown identity were also consistently observed at 90+26 kDa. These bands were successfully blocked by incubation with the immunising peptide.
ab85698 has not yet been referenced specifically in any publications.