Overview

  • Product name

  • Description

    Rabbit polyclonal to HOXD10
  • Host species

    Rabbit
  • Tested applications

    Suitable for: ELISA, WBmore details
  • Species reactivity

    Reacts with: Recombinant fragment
    Predicted to work with: Mouse, Human
  • Immunogen

    Three synthetic peptides (human) conjugated to KLH.

  • Positive control

    • Induced culture of E. coli

Properties

Applications

Our Abpromise guarantee covers the use of ab86014 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA
WB
  • Application notes
    ELISA: Use at an assay dependent dilution. A minimum titer of 1/9000 is determined for one of the three peptides.
    WB: 1/1000. Predicted molecular weight: 38 kDa.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Function

      Sequence-specific transcription factor which is part of a developmental regulatory system that provides cells with specific positional identities on the anterior-posterior axis.
    • Tissue specificity

      Strongly expressed in the adult male and female urogenital tracts.
    • Involvement in disease

      Defects in HOXD10 are a cause of congenital vertical talus (CVT) [MIM:192950]; also known as rocker-bottom foot deformity or congenital convex pes valgus. CVT is a dislocation of the talonavicular joint, with rigid dorsal dislocation of the navicular over the neck of the talus. This condition is usually associated with multiple other congenital deformities and only rarely is an isolated deformity.
    • Sequence similarities

      Belongs to the Abd-B homeobox family.
      Contains 1 homeobox DNA-binding domain.
    • Developmental stage

      Expressed in the developing limb buds.
    • Cellular localization

      Nucleus.
    • Information by UniProt
    • Database links

    • Alternative names

      • AI385591 antibody
      • AI874987 antibody
      • Homeo box 4D antibody
      • Homeo box D10 antibody
      • Homeobox D10 antibody
      • Homeobox protein Hox-4D antibody
      • Homeobox protein Hox-4E antibody
      • Homeobox protein Hox-D10 antibody
      • Hox 4.4 antibody
      • Hox 4.5 antibody
      • Hox 4D antibody
      • Hox 4E antibody
      • Hox 5.3 antibody
      • HOX4D antibody
      • HOX4E antibody
      • HOXD10 antibody
      • HXD10 antibody
      • HXD10_HUMAN antibody
      • OTTMUSP00000017845 antibody
      • RP23-313J15.9 antibody
      see all

    Images

    • All lanes : Anti-HOXD10 antibody (ab86014) at 1/1000 dilution (in 5% non fat milk PBST blocking solution)

      Lane 1 : Uninduced culture (plus anti thio at 1/5000 dilution)
      Lane 2 : Induced culture of E. coli (plus anti thio at 1/5000 dilution)

      Secondary
      All lanes : anti-Rabbit IgG HRP at 1/1000 dilution (in 5% non fat milk PBST blocking solution)

      Predicted band size: 38 kDa
      Observed band size: 52 kDa
      why is the actual band size different from the predicted?


      Exposure time: 1 hour


      Plasmid name: pBAD-DEST49.
      The actual band size differs from the predicted size because it depicts HOXD10 (38.4 kDa) plus a tag (14 kDa)

    References

    ab86014 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab86014.
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