Product nameAnti-HOXD10 antibody
See all HOXD10 primary antibodies
DescriptionRabbit polyclonal to HOXD10
Tested applicationsSuitable for: ELISA, WBmore details
Species reactivityReacts with: Recombinant fragment
Predicted to work with: Mouse, Human
Three synthetic peptides (human) conjugated to KLH.
- Induced culture of E. coli
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage bufferPreservative: 0.01% Thimerosal (merthiolate)
Purification notesCrude serum, final bleed.
Our Abpromise guarantee covers the use of ab86014 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: 1/1000. Predicted molecular weight: 38 kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
FunctionSequence-specific transcription factor which is part of a developmental regulatory system that provides cells with specific positional identities on the anterior-posterior axis.
Tissue specificityStrongly expressed in the adult male and female urogenital tracts.
Involvement in diseaseDefects in HOXD10 are a cause of congenital vertical talus (CVT) [MIM:192950]; also known as rocker-bottom foot deformity or congenital convex pes valgus. CVT is a dislocation of the talonavicular joint, with rigid dorsal dislocation of the navicular over the neck of the talus. This condition is usually associated with multiple other congenital deformities and only rarely is an isolated deformity.
Sequence similaritiesBelongs to the Abd-B homeobox family.
Contains 1 homeobox DNA-binding domain.
Developmental stageExpressed in the developing limb buds.
- Information by UniProt
- AI385591 antibody
- AI874987 antibody
- Homeo box 4D antibody
All lanes : Anti-HOXD10 antibody (ab86014) at 1/1000 dilution (in 5% non fat milk PBST blocking solution)
Lane 1 : Uninduced culture (plus anti thio at 1/5000 dilution)
Lane 2 : Induced culture of E. coli (plus anti thio at 1/5000 dilution)
All lanes : anti-Rabbit IgG HRP at 1/1000 dilution (in 5% non fat milk PBST blocking solution)
Predicted band size: 38 kDa
Observed band size: 52 kDa why is the actual band size different from the predicted?
Exposure time: 1 hour
Plasmid name: pBAD-DEST49.
The actual band size differs from the predicted size because it depicts HOXD10 (38.4 kDa) plus a tag (14 kDa)
ab86014 has not yet been referenced specifically in any publications.