Recombinant Anti-HOXD10 antibody [EPR9374] (ab138508)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR9374] to HOXD10
- Suitable for: Flow Cyt (Intra), WB
- Reacts with: Mouse, Human
Related conjugates and formulations
Overview
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Product name
Anti-HOXD10 antibody [EPR9374]
See all HOXD10 primary antibodies -
Description
Rabbit monoclonal [EPR9374] to HOXD10 -
Host species
Rabbit -
Tested applications
Suitable for: Flow Cyt (Intra), WBmore details
Unsuitable for: ICC/IF,IHC-P or IP -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rat -
Immunogen
Synthetic peptide within Human HOXD10 aa 50-150. The exact sequence is proprietary.
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Positive control
- ECV-304, Fetal heart, Mouse heart, and HeLa whole cell lysate (ab150035), HeLa cells
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
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Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR9374 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab138508 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
Flow Cyt (Intra) |
1/100 - 1/500.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
|
WB |
1/1000 - 1/10000. Predicted molecular weight: 38 kDa.
|
Notes |
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Flow Cyt (Intra)
1/100 - 1/500. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
WB
1/1000 - 1/10000. Predicted molecular weight: 38 kDa. |
Target
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Function
Sequence-specific transcription factor which is part of a developmental regulatory system that provides cells with specific positional identities on the anterior-posterior axis. -
Tissue specificity
Strongly expressed in the adult male and female urogenital tracts. -
Involvement in disease
Defects in HOXD10 are a cause of congenital vertical talus (CVT) [MIM:192950]; also known as rocker-bottom foot deformity or congenital convex pes valgus. CVT is a dislocation of the talonavicular joint, with rigid dorsal dislocation of the navicular over the neck of the talus. This condition is usually associated with multiple other congenital deformities and only rarely is an isolated deformity. -
Sequence similarities
Belongs to the Abd-B homeobox family.
Contains 1 homeobox DNA-binding domain. -
Developmental stage
Expressed in the developing limb buds. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 3236 Human
- Entrez Gene: 15430 Mouse
- Omim: 142984 Human
- SwissProt: P28358 Human
- SwissProt: P28359 Mouse
- Unigene: 123070 Human
- Unigene: 24420 Mouse
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Alternative names
- AI385591 antibody
- AI874987 antibody
- Homeo box 4D antibody
see all
Images
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All lanes : Anti-HOXD10 antibody [EPR9374] (ab138508) at 1/1000 dilution
Lane 1 : ECV-304 cell lysate
Lane 2 : Fetal heart cell lysate
Lane 3 : Mouse heart cell lysate
Lane 4 : HeLa cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 38 kDa -
Intracellular flow cytometrical analysis of permeabilized HeLa cells with ab138508 antibody at a dilution of 1/100 (red) or a rabbit IgG negative (green).
Datasheets and documents
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SDS download
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Datasheet download
References (9)
ab138508 has been referenced in 9 publications.
- Yoo B et al. MiRNA10b-directed nanotherapy effectively targets brain metastases from breast cancer. Sci Rep 11:2844 (2021). PubMed: 33531596
- Sun X et al. MiR-501 promotes tumor proliferation and metastasis by targeting HOXD10 in endometrial cancer. Cell Mol Biol Lett 26:20 (2021). PubMed: 34022794
- Zhang M et al. Regulatory mechanism of miR-525-5p in over-invasion of trophoblast. J Obstet Gynaecol Res 47:679-688 (2021). PubMed: 33258220
- Ma C et al. Circular RNA hsa_circ_0004872 inhibits gastric cancer progression via the miR-224/Smad4/ADAR1 successive regulatory circuit. Mol Cancer 19:157 (2020). PubMed: 33172486
- Zeng Z et al. miR-501 promotes hemangioma progression by targeting HOXD10. Am J Transl Res 11:2439-2446 (2019). PubMed: 31105850
- Li S et al. miR-224 enhances invasion and metastasis by targeting HOXD10 in non-small cell lung cancer cells. Oncol Lett 15:7069-7075 (2018). PubMed: 29731873
- Luan L et al. HOXD10 silencing suppresses human fibroblast-like synoviocyte migration in rheumatoid arthritis via downregulation of the p38/JNK pathway. Exp Ther Med 16:1621-1628 (2018). PubMed: 30186380
- Xu Y et al. Upregulation of long non-coding RNA HIF 1a-anti-sense 1 induced by transforming growth factor-ß-mediated targeting of sirtuin 1 promotes osteoblastic differentiation of human bone marrow stromal cells. Mol Med Rep 12:7233-8 (2015). WB . PubMed: 26460121
- Passadouro M et al. MicroRNA modulation combined with sunitinib as a novel therapeutic strategy for pancreatic cancer. Int J Nanomedicine 9:3203-17 (2014). WB . PubMed: 25061297