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    hoxd11-antibody-5g4-ab55255.pdf

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Epigenetics and Nuclear Signaling Transcription Domain Families Developmental Families HOX
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Anti-HOXD11 antibody [5G4] (ab55255)

  • Datasheet
  • SDS
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Western blot - Anti-HOXD11 antibody [5G4] (ab55255)
  • Immunocytochemistry/ Immunofluorescence - Anti-HOXD11 antibody [5G4] (ab55255)

Key features and details

  • Mouse monoclonal [5G4] to HOXD11
  • Suitable for: WB, ICC/IF
  • Reacts with: Human
  • Isotype: IgG2a

You may also be interested in

Secondary
Product image
Goat Anti-Mouse IgG H&L (HRP) (ab205719)

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Overview

  • Product name

    Anti-HOXD11 antibody [5G4]
    See all HOXD11 primary antibodies
  • Description

    Mouse monoclonal [5G4] to HOXD11
  • Host species

    Mouse
  • Tested applications

    Suitable for: WB, ICC/IFmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant fragment: MNDFDECGQS AASMYLPGCA YYVAPSDFAS KPSFLSQPSS CQMTFPYSSN LAPHVQPVRE VAFRDYGLER AKWPYR, corresponding to amino acids 1-77 of Human HOXD11

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • General notes

    This product was changed from ascites to tissue culture supernatant on 27 June 2019. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Concentration information loading...
  • Purity

    Tissue culture supernatant
  • Purification notes

    Purified from TCS.
  • Clonality

    Monoclonal
  • Clone number

    5G4
  • Isotype

    IgG2a
  • Light chain type

    kappa
  • Research areas

    • Epigenetics and Nuclear Signaling
    • Transcription
    • Domain Families
    • Developmental Families
    • HOX

Associated products

  • Compatible Secondaries

    • Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)
    • Goat Anti-Mouse IgG H&L (HRP) (ab205719)
  • Isotype control

    • Mouse IgG2a, kappa monoclonal [MG2a-53] - Isotype control (ab18415)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab55255 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 36 kDa.
ICC/IF
Use a concentration of 10 µg/ml.
Notes
WB
Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 36 kDa.
ICC/IF
Use a concentration of 10 µg/ml.

Target

  • Function

    Sequence-specific transcription factor which is part of a developmental regulatory system that provides cells with specific positional identities on the anterior-posterior axis.
  • Sequence similarities

    Belongs to the Abd-B homeobox family.
    Contains 1 homeobox DNA-binding domain.
  • Cellular localization

    Nucleus.
  • Target information above from: UniProt accession P31277 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 3237 Human
    • Omim: 142986 Human
    • SwissProt: P31277 Human
    • Unigene: 421136 Human
    • Alternative names

      • Homeo box 4F antibody
      • Homeo box D11 antibody
      • Homeobox D11 antibody
      • Homeobox protein Hox-4F antibody
      • Homeobox protein Hox-D11 antibody
      • Hox-4F antibody
      • HOX4 antibody
      • HOX4F antibody
      • HOXD11 antibody
      • HXD11_HUMAN antibody
      see all

    Images

    • Western blot - Anti-HOXD11 antibody [5G4] (ab55255)
      Western blot - Anti-HOXD11 antibody [5G4] (ab55255)

      HOXD11 antibody (ab55255) at 1ug/lane + K-562 cell lysate at 25ug/lane.

      This image was generated using the ascites version of the product.

    • Immunocytochemistry/ Immunofluorescence - Anti-HOXD11 antibody [5G4] (ab55255)
      Immunocytochemistry/ Immunofluorescence - Anti-HOXD11 antibody [5G4] (ab55255)

      ab55255 at 10 ug/ml staining HOXD11 in human cells by Immunocytochemistry/ Immunofluorescence.

      This image was generated using the ascites version of the product.

    Protocols

    • Western blot protocols
    • Immunocytochemistry & immunofluorescence protocols

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (0)

    Publishing research using ab55255? Please let us know so that we can cite the reference in this datasheet.

    ab55255 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    Show All Reviews Q&A
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    Western blot abreview for Anti-HOXD11 antibody

    Inconclusive
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Mouse Tissue lysate - nuclear (embryonic limb tissue)
    Gel Running Conditions
    Reduced Denaturing
    Loading amount
    40 µg
    Specification
    embryonic limb tissue
    Blocking step
    BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 3µg/mL · Temperature: 23°C
    Read More

    Racheal Paul

    Verified customer

    Submitted Jan 26 2021

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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