• Product name

  • Description

    Rabbit polyclonal to HOXD13
  • Host species

  • Tested applications

    Suitable for: WB, IHC-FoFr, IHC-P, ICC/IFmore details
  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Rat, Chicken, Xenopus laevis
  • Immunogen

    Synthetic peptide corresponding to Human HOXD13 aa 200-300 conjugated to keyhole limpet haemocyanin.
    (Peptide available as ab22466)

  • Positive control

    • In Western Blot, this antibody gave a positive signal against following lysates: HOXD13 Recombinant Protein; Mouse stomach tissue lysate. This antibody gave a positive result when used in the following methanol fixed cell lines: DU145


  • Form

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.02% Sodium Azide
    Constituents: 1% BSA, PBS, pH 7.4
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

  • Isotype

  • Research areas


Our Abpromise guarantee covers the use of ab19866 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 68 kDa (predicted molecular weight: 36 kDa).
IHC-FoFr 1/100 - 1/300.
IHC-P 1/100.
ICC/IF Use a concentration of 5 µg/ml.


  • Relevance

    This gene belongs to the homeobox family of genes. The homeobox genes encode a highly conserved family of transcription factors that play an important role in morphogenesis in all multicellular organisms. Mammals possess four similar homeobox gene clusters, HOXA, HOXB, HOXC and HOXD, located on different chromosomes, consisting of 9 to 11 genes arranged in tandem. This gene is one of several homeobox HOXD genes located in a cluster on chromosome 2. Deletions that remove the entire HOXD gene cluster or the 5' end of this cluster have been associated with severe limb and genital abnormalities. Mutations in this particular gene cause synpolydactyly.
  • Cellular localization

  • Database links

  • Alternative names

    • HOXD13 antibody
    • BDE antibody
    • BDSD antibody
    • Homeo box 4I antibody
    • Homeo box D13 antibody
    • Homeo box protein Hox D13 antibody
    • Homeo box protein HoxD13 antibody
    • Homeobox 4I antibody
    • Homeobox D13 antibody
    • homeobox protein Hox 4I antibody
    • Homeobox protein Hox D13 antibody
    • Homeobox protein Hox-D13 antibody
    • Homeobox protein HoxD13 antibody
    • Homeobox4I antibody
    • HomeoboxD13 antibody
    • Hox 4I antibody
    • HOX D13 antibody
    • Hox-4.8 antibody
    • Hox4I antibody
    • HOXD 13 antibody
    • HoxD13 antibody
    • SPD antibody
    see all


  • ICC/IF image of ab19866 stained DU145 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab19866 at 5 ug/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti-rabbit IgG (H+L) (ab96899) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • ab19866 was tested in IHC of formalin fixed sections from mouse (over two weeks of age) lung. Antigen retrieval was in citrate buffer and blocking was in 1% BSA. The primary antibody was incubated at a dilution of 1/100 overnight at 4 degrees celsius. The secondary antibody was a biotin conjugated anti-rabbit IgG.
  • Anti-HOXD13 antibody (ab19866) at 1 µg/ml + HOXD13 (Human) Recombinant Protein at 0.2 µg

    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Performed under reducing conditions.

    Predicted band size: 36 kDa
    Observed band size: 68 kDa
    why is the actual band size different from the predicted?

    ab19866 was tested on a recombinant GST tagged HOXD13 protein (Human) predicted to run at 65 kDa.


This product has been referenced in:

  • Tatangelo F  et al. Posterior HOX genes and HOTAIR expression in the proximal and distal colon cancer pathogenesis. J Transl Med 16:350 (2018). Read more (PubMed: 30541551) »
  • Aquino G  et al. Deregulation of paralogous 13 HOX genes in oral squamous cell carcinoma. Am J Cancer Res 5:3042-55 (2015). Read more (PubMed: 26693058) »
See all 6 Publications for this product

Customer reviews and Q&As

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Human Tissue sections (Anorectal region)
Anorectal region
Antigen retrieval step
Blocking step
Serum as blocking agent for 15 minute(s) · Concentration: 10%

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Submitted Feb 16 2007

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