Product nameAnti-HP1 alpha antibody
See all HP1 alpha primary antibodies
DescriptionRabbit polyclonal to HP1 alpha
Tested applicationsSuitable for: ICC/IF, WBmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Sheep, Cow, Rhesus monkey
Recombinant fragment within Human HP1 alpha (internal sequence). The exact sequence is proprietary.
Database link: P45973
- ICC/IF: SK-N-SH cells. WB: HeLa whole cell and nuclear lysates.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: PBS, 1% BSA, 20% Glycerol
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab227253 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||1/100 - 1/1000.|
|WB||1/5000 - 1/20000. Predicted molecular weight: 22 kDa.|
FunctionComponent of heterochromatin that recognizes and binds histone H3 tails methylated at 'Lys-9' (H3K9me), leading to epigenetic repression. In contrast, it is excluded from chromatin when 'Tyr-41' of histone H3 is phosphorylated (H3Y41ph). Can interact with lamin-B receptor (LBR). This interaction can contribute to the association of the heterochromatin with the inner nuclear membrane. Involved in the formation of functional kinetochore through interaction with MIS12 complex proteins.
Sequence similaritiesContains 2 chromo domains.
modificationsPhosphorylation of HP1 and LBR may be responsible for some of the alterations in chromatin organization and nuclear structure which occur at various times during the cell cycle (By similarity). Phosphorylated during interphase and possibly hyper-phosphorylated during mitosis.
Cellular localizationNucleus. Chromosome. Chromosome > centromere. Component of centromeric and pericentromeric heterochromatin. Associates with chromosomes during mitosis. Associates specifically with chromatin during metaphase and anaphase.
- Information by UniProt
- Antigen p25 antibody
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All lanes : Anti-HP1 alpha antibody (ab227253) at 1/10000 dilution
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 2 : HeLa (human epithelial cell line from cervix adenocarcinoma) nuclear lysate
Lysates/proteins at 30 µg per lane.
Developed using the ECL technique.
Predicted band size: 22 kDa
12% SDS PAGE
SK-N-SH (human neuroblastoma cell line) cells stained for HP1 alpha (green) using ab227253 (1/500 dilution) in ICC/IF. Cells were fixed in 4% paraformaldehyde at RT for 15 minutes.
Counterstain: Hoechst 33342 (blue).
ab227253 has not yet been referenced specifically in any publications.