Anti-HP1 alpha antibody (ab227253)
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- References
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Overview
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Product name
Anti-HP1 alpha antibody
See all HP1 alpha primary antibodies -
Description
Rabbit polyclonal to HP1 alpha -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat, Sheep, Cow, Rhesus monkey
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Immunogen
Recombinant fragment within Human HP1 alpha (internal sequence). The exact sequence is proprietary.
Database link: P45973 -
Positive control
- ICC/IF: SK-N-SH cells. WB: HeLa whole cell and nuclear lysates.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: PBS, 1% BSA, 20% Glycerol -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
Applications
Our Abpromise guarantee covers the use of ab227253 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC/IF | 1/100 - 1/1000. | |
| WB | 1/5000 - 1/20000. Predicted molecular weight: 22 kDa. |
Target
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Function
Component of heterochromatin that recognizes and binds histone H3 tails methylated at 'Lys-9' (H3K9me), leading to epigenetic repression. In contrast, it is excluded from chromatin when 'Tyr-41' of histone H3 is phosphorylated (H3Y41ph). Can interact with lamin-B receptor (LBR). This interaction can contribute to the association of the heterochromatin with the inner nuclear membrane. Involved in the formation of functional kinetochore through interaction with MIS12 complex proteins. -
Sequence similarities
Contains 2 chromo domains. -
Post-translational
modificationsPhosphorylation of HP1 and LBR may be responsible for some of the alterations in chromatin organization and nuclear structure which occur at various times during the cell cycle (By similarity). Phosphorylated during interphase and possibly hyper-phosphorylated during mitosis.
Ubiquitinated. -
Cellular localization
Nucleus. Chromosome. Chromosome > centromere. Component of centromeric and pericentromeric heterochromatin. Associates with chromosomes during mitosis. Associates specifically with chromatin during metaphase and anaphase. - Information by UniProt
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Database links
- Entrez Gene: 538885 Cow
- Entrez Gene: 23468 Human
- Entrez Gene: 12419 Mouse
- Entrez Gene: 300266 Rat
- Omim: 604478 Human
- SwissProt: P45973 Human
- SwissProt: Q61686 Mouse
- Unigene: 349283 Human
see all -
Alternative names
- Antigen p25 antibody
- CBX5 antibody
- CBX5_HUMAN antibody
see all
Images
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Western blot - Anti-HP1 alpha antibody (ab227253)All lanes : Anti-HP1 alpha antibody (ab227253) at 1/10000 dilution
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 2 : HeLa (human epithelial cell line from cervix adenocarcinoma) nuclear lysate
Lysates/proteins at 30 µg per lane.
Developed using the ECL technique.
Predicted band size: 22 kDa12% SDS PAGE
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Immunocytochemistry/ Immunofluorescence - Anti-HP1 alpha antibody (ab227253)SK-N-SH (human neuroblastoma cell line) cells stained for HP1 alpha (green) using ab227253 (1/500 dilution) in ICC/IF. Cells were fixed in 4% paraformaldehyde at RT for 15 minutes.
Counterstain: Hoechst 33342 (blue).
References
ab227253 has not yet been referenced specifically in any publications.
