Key features and details
- Rabbit polyclonal to HP1 alpha
- Suitable for: IP, IHC-P, WB
- Reacts with: Human
- Isotype: IgG
Product nameAnti-HP1 alpha antibody
See all HP1 alpha primary antibodies
DescriptionRabbit polyclonal to HP1 alpha
Tested applicationsSuitable for: IP, IHC-P, WBmore details
Species reactivityReacts with: Human
Synthetic peptide within Human HP1 alpha aa 150-191. The exact sequence is proprietary. NP_036249.1
Database link: P45973
- WB: HeLa whole cell lysate. IP: HeLa whole cell lysate. IHC-P: Human prostate carcinoma tissue.
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In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7
Preservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate
pH 7 to 8
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab245573 was affinity purified using an epitope specific to HP1 alpha immobilized on solid support.
Our Abpromise guarantee covers the use of ab245573 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IP||Use at 2-5 µg/mg of lysate.|
|IHC-P||1/2000 - 1/10000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|WB||1/1000 - 1/2500. Predicted molecular weight: 22 kDa.|
FunctionComponent of heterochromatin that recognizes and binds histone H3 tails methylated at 'Lys-9' (H3K9me), leading to epigenetic repression. In contrast, it is excluded from chromatin when 'Tyr-41' of histone H3 is phosphorylated (H3Y41ph). Can interact with lamin-B receptor (LBR). This interaction can contribute to the association of the heterochromatin with the inner nuclear membrane. Involved in the formation of functional kinetochore through interaction with MIS12 complex proteins.
Sequence similaritiesContains 2 chromo domains.
modificationsPhosphorylation of HP1 and LBR may be responsible for some of the alterations in chromatin organization and nuclear structure which occur at various times during the cell cycle (By similarity). Phosphorylated during interphase and possibly hyper-phosphorylated during mitosis.
Cellular localizationNucleus. Chromosome. Chromosome > centromere. Component of centromeric and pericentromeric heterochromatin. Associates with chromosomes during mitosis. Associates specifically with chromatin during metaphase and anaphase.
- Information by UniProt
- Antigen p25 antibody
- CBX5 antibody
- CBX5_HUMAN antibody
Formalin-fixed, paraffin-embedded human prostate carcinoma tissue stained for HP1 alpha using ab245573 at 1/5000 dilution in immunohistochemical analysis. Detection: DAB staining.
All lanes : Anti-HP1 alpha antibody (ab245573) at 0.1 µg/ml
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Developed using the ECL technique.
Predicted band size: 22 kDa
Exposure time: 3 minutes
HP1 alpha was immunoprecipitated from HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate (1 mg for IP, 20% of IP loaded) with ab245573 at 3 µg/mg lysate. Western blot was performed from the immunoprecipitate using ab245573 at 1 µg/ml.
Lane 1: ab245573 IP in HeLa whole cell lysate.
Lane 2: Control IgG IP in HeLa whole cell lysate.
Detection: Chemiluminescence with exposure time of 30 seconds.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab245573 has not yet been referenced specifically in any publications.