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Thank you for your email. I am sorry to hear that you have been experiencing problems with this antibody.
I have read the details you have kindly provided and have following further questions for better understanding of the problem;
Questions - ChIP
Sample type, species
Sample preparation (X-ChIP/N-ChIP, cross-linking conditions, DNA fragment size)
Immunoprecipitation conditions (buffer, amount of detergent, primary antibody manufacturer/dilution, incubation time, matrix for isolating antibody complex)
DNA purification (decrosslinking conditions, DNA purification method)
DNA detection method (regions amplified in PCR, microarray, direct sequencing)
Controls used (positive/negative antibodies, positive/negative control regions, no template control in PCR)
Lot number/order number/date of purchase
Sample preparation (cell type, species, lysis buffer, protease inhibitors, phosphatase inhibitors)
Amount of protein loaded
Electrophoresis and transfer conditions
Primary antibody conditions (diluent/dilution, incubation time/temperature)
Secondary antibody conditions (diluent/dilution, incubation time/temperature)
Wash steps and buffer
Controls used (sample, alternative primary ab, no primary)
Thank you very much for your cooperation. I will look forward to hearing from you soon.
Answered on Feb 04 2013