Product nameAnti-HP1 alpha antibody [EPR5776]
See all HP1 alpha primary antibodies
DescriptionRabbit monoclonal [EPR5776] to HP1 alpha
SpecificityThe immunogen used for this product shares 83% homology with CBX1/HP1 beta. Cross-reactivity with this protein has not been confirmed experimentally.
Tested applicationsSuitable for: Flow Cyt, WBmore details
Unsuitable for: ICC/IF,IHC-P or IP
Species reactivityReacts with: Mouse, Rat, Human
Synthetic peptide corresponding to a region within Human HP1 alpha (UniProt P45973).
- SH-SY5Y cells; HeLa, SH-SY5Y, MCF7, A431, C6, RAW 264.7, PC12 and NIH 3T3 cell lysates
Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Storage bufferpH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 40% Glycerol, 9.85% Tris glycine, 50% Tissue culture supernatant
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab133577 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Flow Cyt||1/10 - 1/100.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
|WB||1/1000 - 1/10000. Detects a band of approximately 22 kDa (predicted molecular weight: 22 kDa).|
FunctionComponent of heterochromatin that recognizes and binds histone H3 tails methylated at 'Lys-9' (H3K9me), leading to epigenetic repression. In contrast, it is excluded from chromatin when 'Tyr-41' of histone H3 is phosphorylated (H3Y41ph). Can interact with lamin-B receptor (LBR). This interaction can contribute to the association of the heterochromatin with the inner nuclear membrane. Involved in the formation of functional kinetochore through interaction with MIS12 complex proteins.
Sequence similaritiesContains 2 chromo domains.
modificationsPhosphorylation of HP1 and LBR may be responsible for some of the alterations in chromatin organization and nuclear structure which occur at various times during the cell cycle (By similarity). Phosphorylated during interphase and possibly hyper-phosphorylated during mitosis.
Cellular localizationNucleus. Chromosome. Chromosome > centromere. Component of centromeric and pericentromeric heterochromatin. Associates with chromosomes during mitosis. Associates specifically with chromatin during metaphase and anaphase.
- Information by UniProt
- Antigen p25 antibody
- CBX5 antibody
- CBX5_HUMAN antibody
All lanes : Anti-HP1 alpha antibody [EPR5776] (ab133577) at 1/1000 dilution
Lane 1 : HeLa cell lysate
Lane 2 : SH SY5Y cell lysate
Lane 3 : MCF7 cell lysate
Lane 4 : A431 cell lysate
Lane 5 : C6 cell lysate
Lane 6 : RAW 264.7 cell lysate
Lane 7 : PC12 cell lysate
Lane 8 : NIH 3T3 cell lysate
Lysates/proteins at 10 µg per lane.
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 22 kDa
Flow cytometrric analysis of HPA1 alpha in permeabilized SH SY5Y cells, using ab133577 at a 1/10 dilution (red) or a control rabbit IgG antibody (green).
ab133577 has not yet been referenced specifically in any publications.