• Product name

    Anti-HP1 alpha antibody [EPR5776]
    See all HP1 alpha primary antibodies
  • Description

    Rabbit monoclonal [EPR5776] to HP1 alpha
  • Host species

  • Specificity

    The immunogen used for this product shares 83% homology with CBX1/HP1 beta. Cross-reactivity with this protein has not been confirmed experimentally.
  • Tested applications

    Suitable for: Flow Cyt, WBmore details
    Unsuitable for: ICC/IF,IHC-P or IP
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide corresponding to a region within Human HP1 alpha (UniProt P45973).

  • Positive control

    • SH-SY5Y cells; HeLa, SH-SY5Y, MCF7, A431, C6, RAW 264.7, PC12 and NIH 3T3 cell lysates
  • General notes



    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.



Our Abpromise guarantee covers the use of ab133577 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt 1/10 - 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.


WB 1/1000 - 1/10000. Detects a band of approximately 22 kDa (predicted molecular weight: 22 kDa).
  • Application notes
    Is unsuitable for ICC/IF,IHC-P or IP.
  • Target

    • Function

      Component of heterochromatin that recognizes and binds histone H3 tails methylated at 'Lys-9' (H3K9me), leading to epigenetic repression. In contrast, it is excluded from chromatin when 'Tyr-41' of histone H3 is phosphorylated (H3Y41ph). Can interact with lamin-B receptor (LBR). This interaction can contribute to the association of the heterochromatin with the inner nuclear membrane. Involved in the formation of functional kinetochore through interaction with MIS12 complex proteins.
    • Sequence similarities

      Contains 2 chromo domains.
    • Post-translational

      Phosphorylation of HP1 and LBR may be responsible for some of the alterations in chromatin organization and nuclear structure which occur at various times during the cell cycle (By similarity). Phosphorylated during interphase and possibly hyper-phosphorylated during mitosis.
    • Cellular localization

      Nucleus. Chromosome. Chromosome > centromere. Component of centromeric and pericentromeric heterochromatin. Associates with chromosomes during mitosis. Associates specifically with chromatin during metaphase and anaphase.
    • Information by UniProt
    • Database links

    • Alternative names

      • Antigen p25 antibody
      • CBX5 antibody
      • CBX5_HUMAN antibody
      • CG8409 antibody
      • Chromobox 5 antibody
      • Chromobox homolog 5 (HP1 alpha homolog, Drosophila) antibody
      • Chromobox homolog 5 antibody
      • Chromobox protein homolog 5 antibody
      • Epididymis luminal protein 25 antibody
      • HEL25 antibody
      • Heterochromatin protein 1 alpha antibody
      • Heterochromatin protein 1 antibody
      • Heterochromatin protein 1 homolog alpha antibody
      • HP1 alpha antibody
      • HP1 alpha homolog antibody
      • HP1 antibody
      • HP1A antibody
      • HP1Hs alpha antibody
      • Su(var)205 antibody
      see all


    • All lanes : Anti-HP1 alpha antibody [EPR5776] (ab133577) at 1/1000 dilution

      Lane 1 : HeLa cell lysate
      Lane 2 : SH SY5Y cell lysate
      Lane 3 : MCF7 cell lysate
      Lane 4 : A431 cell lysate
      Lane 5 : C6 cell lysate
      Lane 6 : RAW 264.7 cell lysate
      Lane 7 : PC12 cell lysate
      Lane 8 : NIH 3T3 cell lysate

      Lysates/proteins at 10 µg per lane.

      All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size: 22 kDa

    • Flow cytometrric analysis of HPA1 alpha in permeabilized SH SY5Y cells, using ab133577 at a 1/10 dilution (red) or a control rabbit IgG antibody (green).


    ab133577 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab133577.
    Please use the links above to contact us or submit feedback about this product.

    For licensing inquiries, please contact partnerships@abcam.com

    Sign up