Recombinant Anti-HP1 alpha antibody [EPR5776] - BSA and Azide free (ab248560)
![Western blot - Anti-HP1 alpha antibody [EPR5776] - BSA and Azide free (ab248560)](https://www.abcam.com/ps/products/248/ab248560/Images/ab248560-402701-anti-hp1-alpha-antibody-epr5776-bsa-and-azide-free-western-blot.jpg "Western blot - Anti-HP1 alpha antibody [EPR5776] - BSA and Azide free (ab248560)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR5776] to HP1 alpha - BSA and Azide free
- Suitable for: Flow Cyt, WB
- Reacts with: Mouse, Rat, Human
Overview
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Product name
Anti-HP1 alpha antibody [EPR5776] - BSA and Azide free
See all HP1 alpha primary antibodies -
Description
Rabbit monoclonal [EPR5776] to HP1 alpha - BSA and Azide free -
Host species
Rabbit -
Specificity
The immunogen used for this product shares 83% homology with CBX1/HP1 beta. Cross-reactivity with this protein has not been confirmed experimentally.
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Tested Applications & Species
See all applications and species dataApplication Species Flow Cyt HumanWB Human
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Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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General notes
Ab248560 is the carrier-free version of ab133577. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
ab248560 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Affinity purified -
Clonality
Monoclonal -
Clone number
EPR5776 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Conjugation kits
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab248560 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Tested applications are guaranteed to work and covered by our Abpromise guarantee.
Predicted to work for this combination of applications and species but not guaranteed.
Does not work for this combination of applications and species.
| Application | Species |
|---|---|
| Flow Cyt |
Human
|
| WB |
Human
|
| Application | Abreviews | Notes |
|---|---|---|
| Flow Cyt |
Use at an assay dependent concentration.
|
|
| WB |
Use at an assay dependent concentration. Detects a band of approximately 22 kDa (predicted molecular weight: 22 kDa).
|
| Notes |
|---|
|
Flow Cyt
Use at an assay dependent concentration. |
|
WB
Use at an assay dependent concentration. Detects a band of approximately 22 kDa (predicted molecular weight: 22 kDa). |
Target
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Function
Component of heterochromatin that recognizes and binds histone H3 tails methylated at 'Lys-9' (H3K9me), leading to epigenetic repression. In contrast, it is excluded from chromatin when 'Tyr-41' of histone H3 is phosphorylated (H3Y41ph). Can interact with lamin-B receptor (LBR). This interaction can contribute to the association of the heterochromatin with the inner nuclear membrane. Involved in the formation of functional kinetochore through interaction with MIS12 complex proteins. -
Sequence similarities
Contains 2 chromo domains. -
Post-translational
modificationsPhosphorylation of HP1 and LBR may be responsible for some of the alterations in chromatin organization and nuclear structure which occur at various times during the cell cycle (By similarity). Phosphorylated during interphase and possibly hyper-phosphorylated during mitosis.
Ubiquitinated. -
Cellular localization
Nucleus. Chromosome. Chromosome > centromere. Component of centromeric and pericentromeric heterochromatin. Associates with chromosomes during mitosis. Associates specifically with chromatin during metaphase and anaphase. - Information by UniProt
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Database links
- Entrez Gene: 23468 Human
- Entrez Gene: 12419 Mouse
- Entrez Gene: 300266 Rat
- Omim: 604478 Human
- SwissProt: P45973 Human
- SwissProt: Q61686 Mouse
- Unigene: 349283 Human
- Unigene: 262059 Mouse
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Alternative names
- Antigen p25 antibody
- CBX5 antibody
- CBX5_HUMAN antibody
see all
Images
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Western blot - Anti-HP1 alpha antibody [EPR5776] - BSA and Azide free (ab248560)All lanes : Anti-HP1 alpha antibody [EPR5776] (ab133577) at 1/1000 dilution
Lane 1 : HeLa cell lysate
Lane 2 : SH SY5Y cell lysate
Lane 3 : MCF7 cell lysate
Lane 4 : A431 cell lysate
Lane 5 : C6 cell lysate
Lane 6 : RAW 264.7 cell lysate
Lane 7 : PC12 cell lysate
Lane 8 : NIH 3T3 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 22 kDaThis data was developed using ab133577, the same antibody clone in a different buffer formulation.
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![Flow Cytometry - Anti-HP1 alpha antibody [EPR5776] - BSA and Azide free (ab248560)](https://www.abcam.com/ps/products/248/ab248560/Images/ab248560-1-anti-hp1-alpha-antibody-epr5776-bsa-and-azide-free-flow-cytometry.jpg)
Flow Cytometry - Anti-HP1 alpha antibody [EPR5776] - BSA and Azide free (ab248560) -
![Anti-HP1 alpha antibody [EPR5776] - BSA and Azide free (ab248560)](https://www.abcam.com/ps/products/248/ab248560/Images/ab248560-3-benefits-of-recombinant-antibodies.png)
Anti-HP1 alpha antibody [EPR5776] - BSA and Azide free (ab248560)
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
Certificate of Compliance
References (0)
ab248560 has not yet been referenced specifically in any publications.
