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Epigenetics and Nuclear Signaling Chromatin Binding Proteins Methyl Lysine
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RecombinantRabMAb

Recombinant Anti-HP1 alpha antibody [EPR5776] - BSA and Azide free (ab248560)

  • Datasheet
  • Certificate of Compliance
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Western blot - Anti-HP1 alpha antibody [EPR5776] - BSA and Azide free (ab248560)
  • Flow Cytometry - Anti-HP1 alpha antibody [EPR5776] - BSA and Azide free (ab248560)
  • Anti-HP1 alpha antibody [EPR5776] - BSA and Azide free (ab248560)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR5776] to HP1 alpha - BSA and Azide free
  • Suitable for: Flow Cyt, WB
  • Reacts with: Mouse, Rat, Human

You may also be interested in

Protein
Product image
Recombinant Human HP1 alpha protein (ab124602)
Conjugation
Product image
APC Conjugation Kit - Lightning-Link® (ab201807)

View more associated products

Overview

  • Product name

    Anti-HP1 alpha antibody [EPR5776] - BSA and Azide free
    See all HP1 alpha primary antibodies
  • Description

    Rabbit monoclonal [EPR5776] to HP1 alpha - BSA and Azide free
  • Host species

    Rabbit
  • Specificity

    The immunogen used for this product shares 83% homology with CBX1/HP1 beta. Cross-reactivity with this protein has not been confirmed experimentally.

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    WB
    Human
    See all applications and species data
  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    Ab248560 is the carrier-free version of ab133577. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    ab248560 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C. Do Not Freeze.
  • Storage buffer

    pH: 7.2
    Constituent: PBS
  • Carrier free

    Yes
  • Concentration information loading...
  • Purity

    Affinity purified
  • Clonality

    Monoclonal
  • Clone number

    EPR5776
  • Isotype

    IgG
  • Research areas

    • Epigenetics and Nuclear Signaling
    • Chromatin Binding Proteins
    • Methyl Lysine
    • Tags & Cell Markers
    • Subcellular Markers
    • Nucleus
    • Chromatin

Associated products

  • Alternative Versions

    • Anti-HP1 alpha antibody [EPR5776] (ab133577)
  • Conjugation kits

    • PE / R-Phycoerythrin Conjugation Kit - Lightning-Link® (ab102918)
    • APC Conjugation Kit - Lightning-Link® (ab201807)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control - BSA and Azide Free (ab210849)
  • Positive Controls

    • HeLa nuclear extract lysate (ab14655)
    • MCF7 nuclear extract lysate (ab14860)
    • NIH/3T3 nuclear extract lysate (ab14874)
    • PC-12 nuclear extract lysate (ab14884)
    • RAW 264.7 whole cell lysate (ab7187)
    • A-431 whole cell lysate (ab7909)
  • Recombinant Protein

    • Recombinant Human HP1 alpha protein (ab124602)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab248560 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

Application Species
Flow Cyt
Human
WB
Human
Application Abreviews Notes
Flow Cyt
Use at an assay dependent concentration.
WB
Use at an assay dependent concentration. Detects a band of approximately 22 kDa (predicted molecular weight: 22 kDa).
Notes
Flow Cyt
Use at an assay dependent concentration.
WB
Use at an assay dependent concentration. Detects a band of approximately 22 kDa (predicted molecular weight: 22 kDa).
  • Application notes
    Is unsuitable for ICC/IF,IHC-P or IP.
  • Target

    • Function

      Component of heterochromatin that recognizes and binds histone H3 tails methylated at 'Lys-9' (H3K9me), leading to epigenetic repression. In contrast, it is excluded from chromatin when 'Tyr-41' of histone H3 is phosphorylated (H3Y41ph). Can interact with lamin-B receptor (LBR). This interaction can contribute to the association of the heterochromatin with the inner nuclear membrane. Involved in the formation of functional kinetochore through interaction with MIS12 complex proteins.
    • Sequence similarities

      Contains 2 chromo domains.
    • Post-translational
      modifications

      Phosphorylation of HP1 and LBR may be responsible for some of the alterations in chromatin organization and nuclear structure which occur at various times during the cell cycle (By similarity). Phosphorylated during interphase and possibly hyper-phosphorylated during mitosis.
      Ubiquitinated.
    • Cellular localization

      Nucleus. Chromosome. Chromosome > centromere. Component of centromeric and pericentromeric heterochromatin. Associates with chromosomes during mitosis. Associates specifically with chromatin during metaphase and anaphase.
    • Target information above from: UniProt accession P45973 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 23468 Human
      • Entrez Gene: 12419 Mouse
      • Entrez Gene: 300266 Rat
      • Omim: 604478 Human
      • SwissProt: P45973 Human
      • SwissProt: Q61686 Mouse
      • Unigene: 349283 Human
      • Unigene: 262059 Mouse
      • Alternative names

        • Antigen p25 antibody
        • CBX5 antibody
        • CBX5_HUMAN antibody
        • CG8409 antibody
        • Chromobox 5 antibody
        • Chromobox homolog 5 (HP1 alpha homolog, Drosophila) antibody
        • Chromobox homolog 5 antibody
        • Chromobox protein homolog 5 antibody
        • Epididymis luminal protein 25 antibody
        • HEL25 antibody
        • Heterochromatin protein 1 alpha antibody
        • Heterochromatin protein 1 antibody
        • Heterochromatin protein 1 homolog alpha antibody
        • HP1 alpha antibody
        • HP1 alpha homolog antibody
        • HP1 antibody
        • HP1A antibody
        • HP1Hs alpha antibody
        • Su(var)205 antibody
        see all

      Images

      • Western blot - Anti-HP1 alpha antibody [EPR5776] - BSA and Azide free (ab248560)
        Western blot - Anti-HP1 alpha antibody [EPR5776] - BSA and Azide free (ab248560)
        All lanes : Anti-HP1 alpha antibody [EPR5776] (ab133577) at 1/1000 dilution

        Lane 1 : HeLa cell lysate
        Lane 2 : SH SY5Y cell lysate
        Lane 3 : MCF7 cell lysate
        Lane 4 : A431 cell lysate
        Lane 5 : C6 cell lysate
        Lane 6 : RAW 264.7 cell lysate
        Lane 7 : PC12 cell lysate
        Lane 8 : NIH 3T3 cell lysate

        Lysates/proteins at 10 µg per lane.

        Secondary
        All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

        Predicted band size: 22 kDa



        This data was developed using ab133577, the same antibody clone in a different buffer formulation.

      • Flow Cytometry - Anti-HP1 alpha antibody [EPR5776] - BSA and Azide free (ab248560)
        Flow Cytometry - Anti-HP1 alpha antibody [EPR5776] - BSA and Azide free (ab248560)
        This data was developed using ab133577, the same antibody clone in a different buffer formulation.Flow cytometrric analysis of HPA1 alpha in permeabilized SH SY5Y cells, using ab133577 at a 1/10 dilution (red) or a control rabbit IgG antibody (green).
      • Anti-HP1 alpha antibody [EPR5776] - BSA and Azide free (ab248560)
        Anti-HP1 alpha antibody [EPR5776] - BSA and Azide free (ab248560)

      Protocols

      To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

      Click here to view the general protocols

      Datasheets and documents

      • Datasheet

      Certificate of Compliance

      To download a Certificate of Compliance, please enter your Lot number below:

    • References (0)

      Publishing research using ab248560? Please let us know so that we can cite the reference in this datasheet.

      ab248560 has not yet been referenced specifically in any publications.

      Customer reviews and Q&As

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