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    hp1-alpha-antibody-epr5777-heterochromatin-marker-ab109028.pdf

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Epigenetics and Nuclear Signaling Chromatin Binding Proteins Methyl Lysine
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Validated using a knockout cell lineRecombinantRabMAb

Recombinant Anti-HP1 alpha antibody [EPR5777] - Heterochromatin marker (ab109028)

  • Datasheet
  • SDS
Reviews (8)Q&A (1)References (10)

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Western blot - Anti-HP1 alpha antibody [EPR5777] - Heterochromatin marker (ab109028)
  • Western blot - Anti-HP1 alpha antibody [EPR5777] - Heterochromatin marker (ab109028)
  • Immunoprecipitation - Anti-HP1 alpha antibody [EPR5777] - Heterochromatin marker (ab109028)
  • Immunocytochemistry/ Immunofluorescence - Anti-HP1 alpha antibody [EPR5777] - Heterochromatin marker (ab109028)
  • Flow Cytometry (Intracellular) - Anti-HP1 alpha antibody [EPR5777] - Heterochromatin marker (ab109028)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HP1 alpha antibody [EPR5777] - Heterochromatin marker (ab109028)
  • Immunocytochemistry/ Immunofluorescence - Anti-HP1 alpha antibody [EPR5777] - Heterochromatin marker (ab109028)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HP1 alpha antibody [EPR5777] - Heterochromatin marker (ab109028)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HP1 alpha antibody [EPR5777] - Heterochromatin marker (ab109028)
  • Anti-HP1 alpha antibody [EPR5777] - Heterochromatin marker (ab109028)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR5777] to HP1 alpha - Heterochromatin marker
  • Suitable for: Flow Cyt (Intra), ICC/IF, WB, IP, IHC-P
  • Knockout validated
  • Reacts with: Mouse, Rat, Human

Conjugates logo Related conjugates and formulations

Alexa Fluor® 488 Alexa Fluor® 555 Alexa Fluor® 568 Alexa Fluor® 594 Alexa Fluor® 647 Carrier Free HRP PE

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Anti-SMARCC1/BAF155 antibody [EPR12395] - ChIP Grade (ab172638)

View more associated products

Overview

  • Product name

    Anti-HP1 alpha antibody [EPR5777] - Heterochromatin marker
    See all HP1 alpha primary antibodies
  • Description

    Rabbit monoclonal [EPR5777] to HP1 alpha - Heterochromatin marker
  • Host species

    Rabbit
  • Tested applications

    Suitable for: Flow Cyt (Intra), ICC/IF, WB, IP, IHC-Pmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • ICC/IF: HAP1 and HeLa cells; IP: MCF7 cell lysate; Flow Cyt (intra): HeLa cells; IHC-P: Human breast cancer, rat pancreas, and mouse kidney tissue sections; WB: HeLa, SH-SY5Y whole cell lysates, Rat and mouse brain lysates.
  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 0.05% BSA, 40% Glycerol (glycerin, glycerine), 59% PBS
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR5777
  • Isotype

    IgG
  • Research areas

    • Epigenetics and Nuclear Signaling
    • Chromatin Binding Proteins
    • Methyl Lysine
    • Tags & Cell Markers
    • Subcellular Markers
    • Nucleus
    • Chromatin

Associated products

  • Alternative Versions

    • Alexa Fluor® 488 Anti-HP1 alpha antibody [EPR5777] - Heterochromatin marker (ab185018)
    • HRP Anti-HP1 alpha antibody [EPR5777] - Heterochromatin marker (ab197721)
    • Alexa Fluor® 647 Anti-HP1 alpha antibody [EPR5777] - Heterochromatin marker (ab198391)
    • Alexa Fluor® 568 Anti-HP1 alpha antibody [EPR5777] - Heterochromatin marker (ab202533)
    • Alexa Fluor® 594 Anti-HP1 alpha antibody [EPR5777] - Heterochromatin marker (ab203431)
    • Alexa Fluor® 555 Anti-HP1 alpha antibody [EPR5777] - Heterochromatin marker (ab203432)
    • PE Anti-HP1 alpha antibody [EPR5777] - Heterochromatin marker (ab210585)
    • Anti-HP1 alpha antibody [EPR5777] - BSA and Azide free (ab226049)
  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
    • Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • Recombinant Protein

    • Recombinant Human HP1 alpha protein (ab124602)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab109028 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt (Intra)
1/10 - 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

ICC/IF (4)
1/250.
WB (4)
1/1000. Detects a band of approximately 22 kDa (predicted molecular weight: 22 kDa).

For unpurified use at 1/10000 - 1/50000.

IP
1/10 - 1/100.
IHC-P
1/1000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

See IHC antigen retrieval protocols.

For unpurified use at 1/250 - 1/500.

Notes
Flow Cyt (Intra)
1/10 - 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

ICC/IF
1/250.
WB
1/1000. Detects a band of approximately 22 kDa (predicted molecular weight: 22 kDa).

For unpurified use at 1/10000 - 1/50000.

IP
1/10 - 1/100.
IHC-P
1/1000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

See IHC antigen retrieval protocols.

For unpurified use at 1/250 - 1/500.

Target

  • Function

    Component of heterochromatin that recognizes and binds histone H3 tails methylated at 'Lys-9' (H3K9me), leading to epigenetic repression. In contrast, it is excluded from chromatin when 'Tyr-41' of histone H3 is phosphorylated (H3Y41ph). Can interact with lamin-B receptor (LBR). This interaction can contribute to the association of the heterochromatin with the inner nuclear membrane. Involved in the formation of functional kinetochore through interaction with MIS12 complex proteins.
  • Sequence similarities

    Contains 2 chromo domains.
  • Post-translational
    modifications

    Phosphorylation of HP1 and LBR may be responsible for some of the alterations in chromatin organization and nuclear structure which occur at various times during the cell cycle (By similarity). Phosphorylated during interphase and possibly hyper-phosphorylated during mitosis.
    Ubiquitinated.
  • Cellular localization

    Nucleus. Chromosome. Chromosome > centromere. Component of centromeric and pericentromeric heterochromatin. Associates with chromosomes during mitosis. Associates specifically with chromatin during metaphase and anaphase.
  • Target information above from: UniProt accession P45973 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 23468 Human
    • Entrez Gene: 12419 Mouse
    • Entrez Gene: 300266 Rat
    • Omim: 604478 Human
    • SwissProt: P45973 Human
    • SwissProt: Q61686 Mouse
    • Unigene: 349283 Human
    • Unigene: 262059 Mouse
    • Alternative names

      • Antigen p25 antibody
      • CBX5 antibody
      • CBX5_HUMAN antibody
      • CG8409 antibody
      • Chromobox 5 antibody
      • Chromobox homolog 5 (HP1 alpha homolog, Drosophila) antibody
      • Chromobox homolog 5 antibody
      • Chromobox protein homolog 5 antibody
      • Epididymis luminal protein 25 antibody
      • HEL25 antibody
      • Heterochromatin protein 1 alpha antibody
      • Heterochromatin protein 1 antibody
      • Heterochromatin protein 1 homolog alpha antibody
      • HP1 alpha antibody
      • HP1 alpha homolog antibody
      • HP1 antibody
      • HP1A antibody
      • HP1Hs alpha antibody
      • Su(var)205 antibody
      see all

    Images

    • Western blot - Anti-HP1 alpha antibody [EPR5777] - Heterochromatin marker (ab109028)
      Western blot - Anti-HP1 alpha antibody [EPR5777] - Heterochromatin marker (ab109028)
      All lanes : Anti-HP1 alpha antibody [EPR5777] - Heterochromatin marker (ab109028) at 1/1000 dilution (Purified)

      Lane 1 : HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate
      Lane 2 : SH-SY5Y (Human neuroblastoma epithelial cell) whole cell lysate
      Lane 3 : Mouse brain lysate
      Lane 4 : Rat brain lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

      Predicted band size: 22 kDa



      We are unsure how to define the extra bands.

    • Western blot - Anti-HP1 alpha antibody [EPR5777] - Heterochromatin marker (ab109028)
      Western blot - Anti-HP1 alpha antibody [EPR5777] - Heterochromatin marker (ab109028)

      Lane 1: Wild-type HAP1 cell lysate (20 µg)
      Lane 2: HP1 alpha knockout HAP1 cell lysate (20 µg)
      Lane 3: HeLa cell lysate (20 µg)
      Lane 4: MCF7 cell lysate (20 µg)

      Lanes 1 - 4: Merged signal (red and green). Green - ab109028 observed at 28 kDa. Red - loading control, ab8245, observed at 37 kDa.

      ab109028 was shown to specifically react with HP1 alpha when HP1 alpha knockout samples were used. Wild-type and HP1 alpha knockout samples were subjected to SDS-PAGE. ab109028 and ab8245 (loading control to GAPDH) were diluted 1/1000 and 1/20 000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1/10000 dilution for 1 h at room temperature before imaging.

    • Immunoprecipitation - Anti-HP1 alpha antibody [EPR5777] - Heterochromatin marker (ab109028)
      Immunoprecipitation - Anti-HP1 alpha antibody [EPR5777] - Heterochromatin marker (ab109028)

      Purified ab109028 at 1/30 dilution (2µg) immunoprecipitating HP1 alpha in MCF7 whole cell lysate.
      Lane 1 (input): MCF7 (Human breast adenocarcinoma epithelial cell) whole cell lysate 10µg
      Lane 2 (+): ab109028 + MCF7 whole cell lysate.
      Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab109028 in MCF7 whole cell lysate.
      VeriBlot for IP Detection Reagent (HRP) (ab131366) (1/1000 dilution) was used for Western blotting.
      Blocking Buffer and concentration: 5% NFDM/TBST.
      Diluting buffer and concentration: 5% NFDM/TBST.
      Observed band size: 22 kDa

    • Immunocytochemistry/ Immunofluorescence - Anti-HP1 alpha antibody [EPR5777] - Heterochromatin marker (ab109028)
      Immunocytochemistry/ Immunofluorescence - Anti-HP1 alpha antibody [EPR5777] - Heterochromatin marker (ab109028)
      Immunocytochemistry analysis of HeLa (Human cervix adenocarcinoma epithelial cell) cells labeling HP1 alpha with Purified ab109028 at 1/250 dilution (1.22 µg/mL). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with Ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1/200 dilution (2.5 µg/mL). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1/1000 dilution (2 µg/mL). DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
    • Flow Cytometry (Intracellular) - Anti-HP1 alpha antibody [EPR5777] - Heterochromatin marker (ab109028)
      Flow Cytometry (Intracellular) - Anti-HP1 alpha antibody [EPR5777] - Heterochromatin marker (ab109028)

      Intracellular Flow Cytometry analysis of HeLa (Human cervix adenocarcinoma epithelial cell) cells labeling HP1 alpha with Purified ab109028 at 1/30 dilution (10 µg/mL) (Red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% Methanol. A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) secondary antibody was used at 1/2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).

       

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HP1 alpha antibody [EPR5777] - Heterochromatin marker (ab109028)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HP1 alpha antibody [EPR5777] - Heterochromatin marker (ab109028)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast cancer tissue sections labeling HP1 alpha with Purified ab109028 at 1/1000 dilution (0.31 µg/mL). Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0). Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
    • Immunocytochemistry/ Immunofluorescence - Anti-HP1 alpha antibody [EPR5777] - Heterochromatin marker (ab109028)
      Immunocytochemistry/ Immunofluorescence - Anti-HP1 alpha antibody [EPR5777] - Heterochromatin marker (ab109028)

      ab109028 staining HP1 alpha in wild-type HAP1 cells (top panel) and HP1 alpha knockout HAP1 cells (bottom panel). The cells were fixed with 4% formaldehyde (10min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated with ab109028 at 1/250 dilution and ab195889 at 1/250 dilution (shown in pseudo colour red) overnight at +4°C, followed by a further incubation at room temperature for 1h with a goat secondary antibody to Rabbit IgG (Alexa Fluor® 488) (ab150081) at 2 μg/ml (shown in green). Nuclear DNA was labelled in blue with DAPI.

      Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HP1 alpha antibody [EPR5777] - Heterochromatin marker (ab109028)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HP1 alpha antibody [EPR5777] - Heterochromatin marker (ab109028)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse kidney tissue sections labeling HP1 alpha with Purified ab109028 at 1/1000 dilution (0.31 µg/mL). Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0). Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HP1 alpha antibody [EPR5777] - Heterochromatin marker (ab109028)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HP1 alpha antibody [EPR5777] - Heterochromatin marker (ab109028)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of rat pancreas tissue sections labeling HP1 alpha with Purified ab109028 at 1/1000 dilution (0.31 µg/mL). Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0). Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
    • Anti-HP1 alpha antibody [EPR5777] - Heterochromatin marker (ab109028)
      Anti-HP1 alpha antibody [EPR5777] - Heterochromatin marker (ab109028)

    Protocols

    • Flow cytometry protocols
    • Immunoprecipitation protocols
    • Immunohistochemistry protocols
    • Immunocytochemistry & immunofluorescence protocols
    • Western blot protocols

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (10)

    Publishing research using ab109028? Please let us know so that we can cite the reference in this datasheet.

    ab109028 has been referenced in 10 publications.

    • Strom AR  et al. HP1a is a chromatin crosslinker that controls nuclear and mitotic chromosome mechanics. Elife 10:N/A (2021). PubMed: 34106828
    • Liu X  et al. Time-dependent effect of 1,6-hexanediol on biomolecular condensates and 3D chromatin organization. Genome Biol 22:230 (2021). PubMed: 34404453
    • Lionetti MC  et al. Chromatin and Cytoskeletal Tethering Determine Nuclear Morphology in Progerin-Expressing Cells. Biophys J 118:2319-2332 (2020). PubMed: 32320674
    • Meseure D  et al. Biopathological Significance of PIWI-piRNA Pathway Deregulation in Invasive Breast Carcinomas. Cancers (Basel) 12:N/A (2020). PubMed: 33008024
    • Gomes AP  et al. Dynamic Incorporation of Histone H3 Variants into Chromatin Is Essential for Acquisition of Aggressive Traits and Metastatic Colonization. Cancer Cell 36:402-417.e13 (2019). PubMed: 31564638
    • Damodaran K  et al. Compressive force induces reversible chromatin condensation and cell geometry dependent transcriptional response. Mol Biol Cell N/A:mbcE18040256 (2018). PubMed: 30256731
    • Xie X  et al. ß-Actin-dependent global chromatin organization and gene expression programs control cellular identity. FASEB J 32:1296-1314 (2018). PubMed: 29101221
    • Cappuyns E  et al. Mutations in ADNP affect expression and subcellular localization of the protein. Cell Cycle 17:1068-1075 (2018). PubMed: 29911927
    • Pandey S  et al. A novel MeCP2 acetylation site regulates interaction with ATRX and HDAC1. Genes Cancer 6:408-21 (2015). WB ; Human . PubMed: 26622943
    • Robin JD  et al. Telomere position effect: regulation of gene expression with progressive telomere shortening over long distances. Genes Dev 28:2464-76 (2014). ChIP . PubMed: 25403178

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    1-8 of 8 Abreviews

    Immunocytochemistry/ Immunofluorescence abreview for Anti-HP1 alpha antibody [EPR5777] - Heterochromatin marker

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Mouse Cell (Mouse vascular smooth muscle cells)
    Permeabilization
    Yes - NP40
    Specification
    Mouse vascular smooth muscle cells
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 21°C
    Fixative
    Formaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    Submitted Mar 04 2020

    Western blot abreview for Anti-HP1 alpha antibody [EPR5777] - Heterochromatin marker

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Mouse Cell lysate - nuclear (Mouse vascular smooth muscle cells)
    Gel Running Conditions
    Reduced Denaturing
    Loading amount
    25 µg
    Specification
    Mouse vascular smooth muscle cells
    Blocking step
    Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    Submitted Mar 04 2020

    Immunocytochemistry/ Immunofluorescence abreview for Anti-HP1 alpha antibody [EPR5777] - Heterochromatin marker

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (U2OS)
    Permeabilization
    Yes - NP40
    Specification
    U2OS
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 21°C
    Fixative
    Formaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    Submitted Mar 04 2020

    Western blot abreview for Anti-HP1 alpha antibody [EPR5777] - Heterochromatin marker

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Cell lysate - nuclear (U2OS)
    Gel Running Conditions
    Reduced Denaturing
    Loading amount
    25 µg
    Specification
    U2OS
    Blocking step
    Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    Submitted Mar 04 2020

    Immunocytochemistry/ Immunofluorescence abreview for Anti-HP1 alpha antibody [EPR5777] - Heterochromatin marker

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Mouse Cell (MEF)
    Permeabilization
    Yes - 0.5% Triton 5 minutes
    Specification
    MEF
    Blocking step
    Serum as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
    Fixative
    Paraformaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    Submitted Jan 09 2019

    Immunocytochemistry/ Immunofluorescence abreview for Anti-HP1 alpha antibody [EPR5777] - Heterochromatin marker

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (breast cancer cell lines)
    Permeabilization
    Yes - 0.5% Triton
    Specification
    breast cancer cell lines
    Blocking step
    Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 25°C
    Fixative
    Paraformaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    Submitted Jul 01 2016

    Western blot abreview for Anti-HP1 alpha antibody [EPR5777] - Heterochromatin marker

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (HeLa)
    Gel Running Conditions
    Reduced Denaturing
    Loading amount
    10 µg
    Specification
    HeLa
    Blocking step
    Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    Submitted May 25 2016

    Western blot abreview for Anti-HP1 alpha antibody [EPR5777]

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Cell lysate - other (HeLa)
    Loading amount
    20 µg
    Specification
    HeLa
    Gel Running Conditions
    Reduced Denaturing
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

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    Submitted Jan 10 2013

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