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Western blot abreview for Anti-HP1 alpha antibody [EPR5777] - Heterochromatin marker

Excellent
Abreviews
Go to datasheet (ab109028)
abreview image
Application
Western blot
Sample
Human Cell lysate - whole cell (HeLa)
Gel Running Conditions
Reduced Denaturing
Loading amount
10 µg
Specification
HeLa
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C

Other product details

Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 5% milk
Dilution
1/1000

Secondary antibody

Dilution
1/10000
Name
Non-Abcam antibody was used: Rabbit HRP
Host species: Rabbit
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase

Detection

Detection method
ecl
Exposure
30 second(s)
Bands
Specific: 25 kDa
Positive control
siRNA SMARTPool to HP1a

Additional data

PubMed ID
24018422: View

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Verified customer

Submitted May 25 2016