Key features and details
- Rabbit polyclonal to HP1 gamma/CBX3
- Suitable for: IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
Product nameAnti-HP1 gamma/CBX3 antibody
See all HP1 gamma/CBX3 primary antibodies
DescriptionRabbit polyclonal to HP1 gamma/CBX3
Tested applicationsSuitable for: IHC-Pmore details
Species reactivityReacts with: Mouse, Human
Predicted to work with: Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Dog, Pig, Xenopus laevis, Monkey, Orangutan, Xenopus tropicalis
Synthetic peptide within Human HP1 gamma/CBX3 aa 133-183 (C terminal). The exact sequence is proprietary. NP_009207.2.
Database link: Q13185
- IHC-P: Human breast carcinoma and mouse teratoma tissues.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 6.8
Preservative: 0.09% Sodium azide
Constituents: Tris buffered saline, 0.1% BSA
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab264426 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
FunctionSeems to be involved in transcriptional silencing in heterochromatin-like complexes. Recognizes and binds histone H3 tails methylated at 'Lys-9', leading to epigenetic repression. May contribute to the association of the heterochromatin with the inner nuclear membrane through its interaction with lamin B receptor (LBR). Involved in the formation of functional kinetochore through interaction with MIS12 complex proteins.
Sequence similaritiesContains 2 chromo domains.
modificationsPhosphorylated by PIM1. Phosphorylated during interphase and possibly hyper-phosphorylated during mitosis.
Cellular localizationNucleus. Associates with euchromatin and is largely excluded from constitutive heterochromatin. May be associated with microtubules and mitotic poles during mitosis.
- Information by UniProt
- CBX 3 antibody
- CBX3 antibody
- CBX3_HUMAN antibody
Immunohistochemical analysis of formalin-fixed, paraffin-embedded mouse teratoma tissue labeling HP1 gamma/CBX3 with ab264426 at 1/250 dilution. Detection: DAB.
Immunohistochemical analysis of formalin-fixed, paraffin-embedded human breast carcinoma tissue labeling HP1 gamma/CBX3 with ab264426 at 1/250 dilution. Detection: DAB.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab264426 has not yet been referenced specifically in any publications.