Overview

  • Product name

    Anti-HP1 gamma/CBX3 antibody (Biotin)
    See all HP1 gamma/CBX3 primary antibodies
  • Description

    Goat polyclonal to HP1 gamma/CBX3 (Biotin)
  • Host species

    Goat
  • Conjugation

    Biotin
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Orangutan
  • Immunogen

    Synthetic peptide corresponding to Human HP1 gamma/CBX3 aa 74-87 (internal sequence) (Cysteine residue). (NP_009207.2)
    Sequence:

    C-EAFLNSQKAGKEKD


    Database link: Q13185

  • Positive control

    • WB: NIH/3T3 cell lysate.
  • General notes

     This product was previously labelled as HP1 gamma

     

Properties

Applications

Our Abpromise guarantee covers the use of ab240481 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.1 - 0.3 µg/ml. Predicted molecular weight: 20 kDa.

Target

  • Function

    Seems to be involved in transcriptional silencing in heterochromatin-like complexes. Recognizes and binds histone H3 tails methylated at 'Lys-9', leading to epigenetic repression. May contribute to the association of the heterochromatin with the inner nuclear membrane through its interaction with lamin B receptor (LBR). Involved in the formation of functional kinetochore through interaction with MIS12 complex proteins.
  • Sequence similarities

    Contains 2 chromo domains.
  • Post-translational
    modifications

    Phosphorylated by PIM1. Phosphorylated during interphase and possibly hyper-phosphorylated during mitosis.
  • Cellular localization

    Nucleus. Associates with euchromatin and is largely excluded from constitutive heterochromatin. May be associated with microtubules and mitotic poles during mitosis.
  • Information by UniProt
  • Database links

  • Alternative names

    • CBX 3 antibody
    • CBX3 antibody
    • CBX3_HUMAN antibody
    • Chromobox homolog 3 (HP1 gamma homolog, Drosophila) antibody
    • Chromobox homolog 3 antibody
    • Chromobox protein homolog 3 antibody
    • GAMMA antibody
    • HECH antibody
    • Heterochromatin like protein 1 antibody
    • Heterochromatin protein 1 homolog gamma antibody
    • Heterochromatin protein HP1 gamma antibody
    • HP1 gamma antibody
    • HP1 gamma homolog antibody
    • HP1Hs gamma antibody
    • Modifier 2 protein antibody
    see all

Images

  • Anti-HP1 gamma/CBX3 antibody (Biotin) (ab240481) at 0.3 µg/ml + NIH/3T3 (mouse embryo fibroblast cell line) cell lysate in RIPA buffer at 35 µg

    Secondary
    streptavidin-HRP

    Developed using the ECL technique.

    Predicted band size: 20 kDa



    Primary incubation for 1 hour.

    Staining mirrors that observed with the parental, non-biotinylated version of the antibody.

References

ab240481 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab240481.
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