Overview

  • Product name

    Anti-HP1 gamma/CBX3 antibody [EPR10465(B)]
    See all HP1 gamma/CBX3 primary antibodies
  • Description

    Rabbit monoclonal [EPR10465(B)] to HP1 gamma/CBX3
  • Host species

    Rabbit
  • Specificity

    The mouse and rat recommendation is based on the WB results. We do not guarantee IHC-P for mouse and rat.

  • Tested applications

    Suitable for: WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide corresponding to Human HP1 gamma/CBX3.
    Database link: Q13185

  • Positive control

    • WB: NIH/3T3, HeLa, Molt-4 and HepG2 cell lysates, Mouse and Rat brain lysates; IHC-P: Human breast carcinoma tissue, human gastric cancer, Human normal breast tissue.
  • General notes

     This product was previously labelled as HP1 gamma

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab154871 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 20 kDa.
IHC-P 1/4000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

For unpurified use at 1/50 - 1/100.

See IHC antigen retrieval protocols.

The mouse and rat recommendation is based on the WB results. We do not guarantee IHC-P for mouse and rat.

Target

  • Function

    Seems to be involved in transcriptional silencing in heterochromatin-like complexes. Recognizes and binds histone H3 tails methylated at 'Lys-9', leading to epigenetic repression. May contribute to the association of the heterochromatin with the inner nuclear membrane through its interaction with lamin B receptor (LBR). Involved in the formation of functional kinetochore through interaction with MIS12 complex proteins.
  • Sequence similarities

    Contains 2 chromo domains.
  • Post-translational
    modifications

    Phosphorylated by PIM1. Phosphorylated during interphase and possibly hyper-phosphorylated during mitosis.
  • Cellular localization

    Nucleus. Associates with euchromatin and is largely excluded from constitutive heterochromatin. May be associated with microtubules and mitotic poles during mitosis.
  • Information by UniProt
  • Database links

  • Alternative names

    • CBX 3 antibody
    • CBX3 antibody
    • CBX3_HUMAN antibody
    • Chromobox homolog 3 (HP1 gamma homolog, Drosophila) antibody
    • Chromobox homolog 3 antibody
    • Chromobox protein homolog 3 antibody
    • GAMMA antibody
    • HECH antibody
    • Heterochromatin like protein 1 antibody
    • Heterochromatin protein 1 homolog gamma antibody
    • Heterochromatin protein HP1 gamma antibody
    • HP1 gamma antibody
    • HP1 gamma homolog antibody
    • HP1Hs gamma antibody
    • Modifier 2 protein antibody
    see all

Images

  • Lane 1: Wild-type HAP1 cell lysate (20 µg)
    Lane 2: HP1 gamma/CBX3 knockout HAP1 cell lysate (20 µg)
    Lane 3: NIH3T3 cell lysate (20 µg)
    Lane 4: HeLa cell lysate (20 µg)
    Lanes 1 - 4: Merged signal (red and green). Green - ab154871 observed at 24 kDa. Red - loading control, ab8245, observed at 37 kDa.

    ab154871was shown to recognize HP1 gamma/CBX3 when HP1 gamma/CBX3 knockout samples were used, along with additional cross-reactive bands. Wild-type and HP1 gamma/CBX3 knockout samples were subjected to SDS-PAGE. ab154871 and ab8245 (loading control to GAPDH) were both diluted to 1/500 and 1/10,000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776 secondary antibodies at 1/10,000 dilution for 1 hour at room temperature before imaging.

  • All lanes : Anti-HP1 gamma/CBX3 antibody [EPR10465(B)] (ab154871) at 1/5000 dilution (Purified)

    Lane 1 : HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysates
    Lane 2 : Mouse brain lysates
    Lane 3 : Rat brain lysates

    Lysates/proteins at 15 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 20 kDa
    Observed band size: 21 kDa
    why is the actual band size different from the predicted?

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human gastric cancer tissue sections labeling HP1 gamma/CBX3 with purified ab154871 at 1:4000 dilution (0.29 µg/ml). Heat mediated antigen retrieval was performed Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
  • All lanes : Anti-HP1 gamma/CBX3 antibody [EPR10465(B)] (ab154871) at 1/1000 dilution

    Lane 1 : NIH/3T3 cell lysate
    Lane 2 : HeLa cell lysate
    Lane 3 : Molt-4 cell lysate
    Lane 4 : HepG2 cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat anti-rabbit HRP at 1/2000 dilution

    Predicted band size: 20 kDa

  • Immunohistochemical analysis of paraffin-embedded Human breast carcinoma tissue labeling HP1 gamma/CBX3 with ab154871 at 1/50 dilution.

  • Immunohistochemical analysis of paraffin-embedded Human normal breast tissue labeling HP1 gamma/CBX3 with ab154871 at 1/50 dilution.

  • Immunohistochemical analysis of paraffin embedded Human normal kidney tissue using ab154871 showing +ve staining.

  • Immunohistochemical analysis of paraffin embedded Human thyroid gland carcinoma tissue using ab154871 showing +ve staining.

  • Immunohistochemical analysis of paraffin embedded Human normal uterus tissue using ab154871 showing +ve staining.

  • Immunohistochemical analysis of paraffin embedded Human ovarian carcinoma tissue using ab154871 showing +ve staining.

References

This product has been referenced in:

  • Yang G  et al. The histone H3K9 methyltransferase SUV39H links SIRT1 repression to myocardial infarction. Nat Commun 8:14941 (2017). ChIP . Read more (PubMed: 28361889) »
See 1 Publication for this product

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