Overview

  • Product name

    Anti-HP1 gamma/CBX3 antibody [EPR10466(B)]
    See all HP1 gamma/CBX3 primary antibodies
  • Description

    Rabbit monoclonal [EPR10466(B)] to HP1 gamma/CBX3
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, Flow Cytmore details
    Unsuitable for: ICC/IF,IHC-P or IP
  • Species reactivity

    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide within Human HP1 gamma/CBX3. The exact sequence is proprietary.

  • Positive control

    • HepG2, MOLT4 and HeLa cell lysates; HeLa cells.
  • General notes

    Rat: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.

    Mouse: In our hands this product works best in WB using cell lines rather than tissue lysates.

     This product was previously labelled as HP1 gamma

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab154195 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 20 kDa.
Flow Cyt 1/10 - 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 

  • Application notes
    Is unsuitable for ICC/IF,IHC-P or IP.
  • Target

    • Function

      Seems to be involved in transcriptional silencing in heterochromatin-like complexes. Recognizes and binds histone H3 tails methylated at 'Lys-9', leading to epigenetic repression. May contribute to the association of the heterochromatin with the inner nuclear membrane through its interaction with lamin B receptor (LBR). Involved in the formation of functional kinetochore through interaction with MIS12 complex proteins.
    • Sequence similarities

      Contains 2 chromo domains.
    • Post-translational
      modifications

      Phosphorylated by PIM1. Phosphorylated during interphase and possibly hyper-phosphorylated during mitosis.
    • Cellular localization

      Nucleus. Associates with euchromatin and is largely excluded from constitutive heterochromatin. May be associated with microtubules and mitotic poles during mitosis.
    • Information by UniProt
    • Database links

    • Alternative names

      • CBX 3 antibody
      • CBX3 antibody
      • CBX3_HUMAN antibody
      • Chromobox homolog 3 (HP1 gamma homolog, Drosophila) antibody
      • Chromobox homolog 3 antibody
      • Chromobox protein homolog 3 antibody
      • GAMMA antibody
      • HECH antibody
      • Heterochromatin like protein 1 antibody
      • Heterochromatin protein 1 homolog gamma antibody
      • Heterochromatin protein HP1 gamma antibody
      • HP1 gamma antibody
      • HP1 gamma homolog antibody
      • HP1Hs gamma antibody
      • Modifier 2 protein antibody
      see all

    Images

    • Lane 1: Wild-type HAP1 cell lysate (20 µg)
      Lane 2: HP1 gamma/CBX3 knockout HAP1 cell lysate (20 µg)
      Lane 3: NIH3T3 cell lysate (20 µg)
      Lane 4: HeLa cell lysate (20 µg)
      Lanes 1 - 4: Merged signal (red and green). Green - ab154195 observed at 24 kDa. Red - loading control, ab8245, observed at 37 kDa.
      ab154195 was shown to recognize HP1 gamma/CBX3 when HP1 gamma/CBX3 knockout samples were used, along with additional cross-reactive bands. Wild-type and HP1 gamma/CBX3 knockout samples were subjected to SDS-PAGE. ab154195 and ab8245 (loading control to GAPDH) were diluted at 1/1000 and 1/10000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776 secondary antibodies at 1/10000 dilution for 1 h at room temperature before imaging.

    • All lanes : Anti-HP1 gamma/CBX3 antibody [EPR10466(B)] (ab154195) at 1/1000 dilution

      Lane 1 : HepG2 cell lysate
      Lane 2 : MOLT4 cell lysate
      Lane 3 : HeLa cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat anti-rabbit HRP at 1/2000 dilution

      Predicted band size: 20 kDa

    • Flow cytometric analysis of permeabilized HeLa cells labeling HP1 gamma/CBX3 with ab154195 at 1/10 dilution (red) compared with a rabbit IgG negative control antibody (green).

    References

    ab154195 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab154195.
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    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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