Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR19802-202] to HP1 gamma/CBX3 (citrulline R108)
- Suitable for: WB, Dot blot, IP, Flow Cyt
- Knockout validated
- Reacts with: Mouse, Rat, Human
Product nameAnti-HP1 gamma/CBX3 (citrulline R108) antibody [EPR19802-202]
See all HP1 gamma/CBX3 primary antibodies
DescriptionRabbit monoclonal [EPR19802-202] to HP1 gamma/CBX3 (citrulline R108)
Tested applicationsSuitable for: WB, Dot blot, IP, Flow Cytmore details
Species reactivityReacts with: Mouse, Rat, Human
Synthetic peptide corresponding to Mouse HP1 gamma/CBX3 aa 100 to the C-terminus (citrulline R108).
Database link: P23198
- WB: HEK-293 transfected with GFP-tagged PADI2 or PADI4 (WT) cell lysates. IP: HEK-293 transfected with PADI4 cell lysate treated with calcium chloride and Ionomycin. Flow Cyt: HEK-293 transfected with PADI4 cells treated with calcium chloride and Ionomycin.
This product was previously labelled as HP1 gamma
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 0.05% BSA, 40% Glycerol (glycerin, glycerine), PBS
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab202107 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000. Detects a band of approximately 21 kDa (predicted molecular weight: 21 kDa).|
FunctionSeems to be involved in transcriptional silencing in heterochromatin-like complexes. Recognizes and binds histone H3 tails methylated at 'Lys-9', leading to epigenetic repression. May contribute to the association of the heterochromatin with the inner nuclear membrane through its interaction with lamin B receptor (LBR). Involved in the formation of functional kinetochore through interaction with MIS12 complex proteins.
Sequence similaritiesContains 2 chromo domains.
modificationsPhosphorylated by PIM1. Phosphorylated during interphase and possibly hyper-phosphorylated during mitosis.
Cellular localizationNucleus. Associates with euchromatin and is largely excluded from constitutive heterochromatin. May be associated with microtubules and mitotic poles during mitosis.
- Information by UniProt
- CBX 3 antibody
- CBX3 antibody
- CBX3_HUMAN antibody
Dot blot analysis of HP1 gamma/CBX3 (citrulline R108) labeled with ab202107 at 1/1000 dilution.
Lane 1: HP1 gamma/CBX3 (citrulline R108) peptide;
Lane 2: HP1 gamma/CBX3 (non-citrulline) peptide;
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution was used as secondary antibody.
Blocking/Dilution buffer: 5% NFDM/TBST.
Exposure time: 3 minutes.
Lane 1: Wild type HAP1 whole cell lysate (20 µg)
Lane 2: HP1 gamma/CBX3 knockout HAP1 whole cell lysate (20 µg)
Lane 3: HUVEC (umbilicalvein/vascular endothelium) whole cell lysate (20 µg)
Lane 4: HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate (20 µg)
Lanes 1 - 4: Merged signal (red and green). Green - ab202107 observed at 23 kDa. Red - loading control, ab8245, observed at 37 kDa.
ab202107 was shown to specifically react with HP1 gamma/CBX3 when HP1 gamma/CBX3 knockout samples were used. Wild-type and HP1 gamma/CBX3 knockout samples were subjected to SDS-PAGE. Antibodies ab202107 and ab8245 (Mouse anti GAPDH loading control) were incubated overnight at 4°C at 1000 dilution and 1/10000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.
All lanes : Anti-HP1 gamma/CBX3 (citrulline R108) antibody [EPR19802-202] (ab202107) at 1/1000 dilution
Lanes 1 & 3 : HEK-293 (human epithelial cell line from embryonic kidney) transfected with a control vector containing GFP tag treated with 10 mM calcium chloride and 10 µM Ionomycin for 2 hours, whole cell lysate
Lane 2 : HEK-293 (human epithelial cell line from embryonic kidney) transfected with GFP-tagged PADI4 (WT) expression vector, treated with 10 mM calcium chloride and 10 µM Ionomycin for 2 hours, whole cell lysate
Lane 4 : HEK-293 (human epithelial cell line from embryonic kidney) transfected with GFP-tagged PADI2 (WT) expression vector treated with 10 mM calcium chloride and 10 µM Ionomycin for 2 hours, whole cell lysate
Lysates/proteins at 20 µg per lane.
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 21 kDa
Observed band size: 21 kDa
Blocking/Dilution buffer: 5% NFDM/TBST.
Exposure time: Lanes 1-2: 2 seconds; Lanes 3-4: 6 seconds.
Flow cytometric analysis of 4% paraformaldehyde-fixed HEK-293 (human epithelial cell line from embryonic kidney) cells transfected with GFP-tagged PADI4 (WT) treated with 10 mM CaCl2 and 10 μM Ionomycin labeling HP1 gamma/CBX3 (citrulline R108) with ab202107 at 1/3000 dilution (right panel) compared with a rabbit monoclonal IgG isotype control (ab172730) (left panel). Goat Anti-Rabbit IgG H+L (Alexa Fluor® 647) (ab150079) was used as the secondary antibody.
Only CaCl2 and Ionomycin treated, GFP positive cell population result in a positive signal (right panel, Q2).
HP1 gamma/CBX3 (citrulline R108) was immunoprecipitated from 0.35 mg HEK-293 (human epithelial cell line from embryonic kidney) transfected with GFP-tagged PADI4 cell lysate treated with 10 mM CaCl2 and 10 μM Ionomycin for 2 hours with ab202107 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab202107 at a dilution of 1/2000. VeriBlot for IP Detection Reagent (HRP) (ab131366) was used for detection at 1/10000 dilution.
Lane 1: HEK-293 transfected with GFP-tagged PADI4 (WT) expression vector whole cell lysate treated with 10 mM CaCl2 and 10 μM Ionomycin for 2 hours 10 μg (Input).
Lane 2: ab202107 IP in HEK-293 transfected with GFP-tagged PADI4 (WT) expression vector whole cell lysate treated with 10 mM CaCl2 and 10 μM Ionomycin for 2 hours.
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab202107 in HEK-293 transfected with GFP-tagged PADI4 (WT) expression vector whole cell lysate treated with 10 mM CaCl2 and 10 μM Ionomycin for 2 hours.
Exposure time: 10 seconds.
ab202107 has not yet been referenced specifically in any publications.