Anti-HP1BP3 antibody (ab224754)
Key features and details
- Rabbit polyclonal to HP1BP3
- Suitable for: IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-HP1BP3 antibody
See all HP1BP3 primary antibodies -
Description
Rabbit polyclonal to HP1BP3 -
Host species
Rabbit -
Tested applications
Suitable for: IHC-Pmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat, Cow -
Immunogen
Recombinant fragment corresponding to Human HP1BP3 aa 150-300.
Database link: Q5SSJ5 -
Positive control
- IHC-P: Human pancreas tissue.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), PBS -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab224754 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P |
1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Notes |
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IHC-P
1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
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Function
Component of heterochromatin that maintains heterochromatin integrity during G1/S progression and regulates the duration of G1 phase to critically influence cell proliferative capacity (PubMed:24830416). Mediates chromatin condensation during hypoxia, leading to increased tumor cell viability, radio-resistance, chemo-resistance and self-renewal(PubMed:25100860). -
Sequence similarities
Contains 3 H15 (linker histone H1/H5 globular) domains. -
Domain
A central region that included the first H15 (linker histone H1/H5 globular) domain binds at the entry/exit site of the nucleosomal DNA. -
Cellular localization
Nucleus. Chromosome. localized in nuclei but not in nucleoli in interphase. Colocalized with chromosomes in mitosis, with a gradually increased during G1 progression and a maximum level during late G1 phase (G1/S). - Information by UniProt
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Database links
- Entrez Gene: 510194 Cow
- Entrez Gene: 50809 Human
- Entrez Gene: 15441 Mouse
- Entrez Gene: 313647 Rat
- SwissProt: Q08DU9 Cow
- SwissProt: Q5SSJ5 Human
- SwissProt: Q3TEA8 Mouse
- SwissProt: Q6P747 Rat
see all -
Alternative names
- Heterochromatin protein 1, binding protein 3 antibody
- Heterochromatin protein 1-binding protein 3 antibody
- HP1 BP74 antibody
see all
Images
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab224754 has not yet been referenced specifically in any publications.