Key features and details
- Mouse polyclonal to HP1BP3
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Product nameAnti-HP1BP3 antibody
See all HP1BP3 primary antibodies
DescriptionMouse polyclonal to HP1BP3
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Human
Full length human HP1BP3 protein, (NP_057371, 553 a.a)
- HP1BP3 transfected 293T lysate.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage bufferpH: 7.20
Constituent: 2.68% PBS
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab68719 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/1000. Detects a band of approximately 75 kDa (predicted molecular weight: 61 kDa).|
FunctionComponent of heterochromatin that maintains heterochromatin integrity during G1/S progression and regulates the duration of G1 phase to critically influence cell proliferative capacity (PubMed:24830416). Mediates chromatin condensation during hypoxia, leading to increased tumor cell viability, radio-resistance, chemo-resistance and self-renewal(PubMed:25100860).
Sequence similaritiesContains 3 H15 (linker histone H1/H5 globular) domains.
DomainA central region that included the first H15 (linker histone H1/H5 globular) domain binds at the entry/exit site of the nucleosomal DNA.
Cellular localizationNucleus. Chromosome. localized in nuclei but not in nucleoli in interphase. Colocalized with chromosomes in mitosis, with a gradually increased during G1 progression and a maximum level during late G1 phase (G1/S).
- Information by UniProt
- Heterochromatin protein 1, binding protein 3 antibody
- Heterochromatin protein 1-binding protein 3 antibody
- HP1 BP74 antibody
All lanes : Anti-HP1BP3 antibody (ab68719) at 1/500 dilution
Lane 1 : HP1BP3 transfected 293T lysate
Lane 2 : Non-transfected 293T lysate
Lysates/proteins at 25 µg per lane.
All lanes : Goat Anti-Mouse IgG (H&L)-HRP at 1/2500 dilution
Predicted band size: 61 kDa
Observed band size: 75 kDa why is the actual band size different from the predicted?
ab68719 has not yet been referenced specifically in any publications.