Key features and details
- Mouse monoclonal [OTI3F11] to HPCAL4
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG2a
Product nameAnti-HPCAL4 antibody [OTI3F11]
DescriptionMouse monoclonal [OTI3F11] to HPCAL4
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Cow, Orangutan
Recombinant full length protein corresponding to Human HPCAL4 aa 2-191. Produced in E.coli (NP_057341).
GKTNSKLAPEVLEDLVQNTEFSEQELKQWYKGFLKDCPSGILNLEEFQQL YIKFFPYGDASKFAQHAFRTFDKNGDGTIDFREFICALSVTSRGSFEQKL NWAFEMYDLDGDGRITRLEMLEIIEAIYKMVGTVIMMRMNQDGLTPQQRV DKIFKKMDQDKDDQITLEEFKEAAKSDPSIVLLLQCDMQK
Database link: Q9UM19
- WB: HEK-293T cell lysates transfected with pCMV6-ENTRY HPCAL4 cDNA for 48 hrs.
Clone OTI3F11 (formerly 3F11).
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.30
Preservative: 0.02% Sodium azide
Constituents: PBS, 1% BSA, 50% Glycerol
Concentration information loading...
Purification notesPurified from cell culture supernatant.
Our Abpromise guarantee covers the use of ab236390 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/2000. Predicted molecular weight: 22 kDa.|
FunctionMay be involved in the calcium-dependent regulation of rhodopsin phosphorylation.
Sequence similaritiesBelongs to the recoverin family.
Contains 4 EF-hand domains.
- Information by UniProt
- Hippocalcin like 4 antibody
- Hippocalcin like protein 4 antibody
- Hippocalcin-like protein 4 antibody
All lanes : Anti-HPCAL4 antibody [OTI3F11] (ab236390) at 1/2000 dilution
Lane 1 : HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) cell lysates transfected with pCMV6-ENTRY control cDNA for 48 hrs
Lane 2 : HEK-293T cell lysates transfected with pCMV6-ENTRY HPCAL4 cDNA for 48 hrs
Lysates/proteins at 5 µg per lane.
Predicted band size: 22 kDa
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab236390 has not yet been referenced specifically in any publications.