Overview

  • Product name

    Anti-HPD antibody [EPR5297]
    See all HPD primary antibodies
  • Description

    Rabbit monoclonal [EPR5297] to HPD
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-Pmore details
    Unsuitable for: Flow Cyt,ICC/IF or IP
  • Species reactivity

    Reacts with: Rat, Human
  • Immunogen

    Synthetic peptide corresponding to residues in Human HPD (UniProt ID: P32754)

  • Positive control

    • HepG2 and Fetal liver lysates. Human kidney tissue.
  • General notes

     

    Mouse: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab133515 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 45 kDa.
IHC-P 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

For unpurified use at 1/250-1/50

See IHC antigen retrieval protocols.

The rat recommendation is based on the WB results. This antibody may not be suitable for IHC with rat samples.

  • Application notes
    Is unsuitable for Flow Cyt,ICC/IF or IP.
  • Target

    • Function

      Key enzyme in the degradation of tyrosine.
    • Pathway

      Amino-acid degradation; L-phenylalanine degradation; acetoacetate and fumarate from L-phenylalanine: step 3/6.
    • Involvement in disease

      Tyrosinemia 3
      Hawkinsinuria
    • Sequence similarities

      Belongs to the 4HPPD family.
    • Information by UniProt
    • Database links

    • Alternative names

      • 4 HPPD antibody
      • 4 hydroxyphenylpyruvate dioxygenase antibody
      • 4 hydroxyphenylpyruvic acid oxidase antibody
      • 4-hydroxyphenylpyruvate dioxygenase antibody
      • 4-hydroxyphenylpyruvic acid oxidase antibody
      • 4HPPD antibody
      • GLOD3 antibody
      • glyoxalase domain containing 3 antibody
      • HPD antibody
      • HPPD_HUMAN antibody
      • HPPDase antibody
      • PPD antibody
      see all

    Images

    • Anti-HPD antibody [EPR5297] (ab133515) at 1/1000 dilution (purified) + HepG2 (Human hepatocellular carcinoma epithelial cell) whole cell lysates at 20 µg

      Secondary
      Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

      Predicted band size: 45 kDa
      Observed band size: 45 kDa



      Blocking and diluting buffer: 5% NFDM/TBST

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human liver tissue sections labeling HPD with purified ab133515 at 1:2000 dilution (0.76 μg/ml). Heat mediated antigen retrieval was performed using Perform heat mediated antigen retrieval using EDTA Buffer, pH9.0. Tissue was counterstained with Hematoxylin. ImmunoHistoProbe one step HRP Polymer (ready to use) secondary antibody was used at 1:0 dilution. PBS instead of the primary antibody was used as the negative control.

    • Anti-HPD antibody [EPR5297] (ab133515) at 1/5000 dilution (purified) + Rat liver lysates at 20 µg

      Secondary
      Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

      Predicted band size: 45 kDa



      Blocking and diluting buffer: 5% NFDM/TBST

    • All lanes : Anti-HPD antibody [EPR5297] (ab133515) at 1/1000 dilution (unpurified)

      Lane 1 : HepG2 lysate
      Lane 2 : Fetal liver lysate
      Lane 3 : Human kidney lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat-anti-rabbit HRP at 1/2000 dilution

      Developed using the ECL technique.

      Predicted band size: 45 kDa

    • Immunohistochemical analysis of paraffin-embedded Human kidney tissue labelling HPD with unpurified ab133515 at 1/250 dilution.

    • Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD

    References

    ab133515 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab133515.
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    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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