Validated using a knockout cell line
Recombinant
RabMAb

Recombinant Anti-HPRT antibody [EPR5298] - BSA and Azide free (ab240051)

Overview

  • Product name

    Anti-HPRT antibody [EPR5298] - BSA and Azide free
    See all HPRT primary antibodies
  • Description

    Rabbit monoclonal [EPR5298] to HPRT - BSA and Azide free
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IP, WBmore details
    Unsuitable for: Flow Cyt,ICC/IF or IHC-P
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human HPRT aa 50-150. The exact sequence is proprietary.

  • General notes

    Ab240051 is the carrier-free version of ab133242. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    ab240051 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab240051 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP Use at an assay dependent concentration.
WB Use at an assay dependent concentration. Predicted molecular weight: 25 kDa.
  • Application notes
    Is unsuitable for Flow Cyt,ICC/IF or IHC-P.
  • Target

    • Function

      Converts guanine to guanosine monophosphate, and hypoxanthine to inosine monophosphate. Transfers the 5-phosphoribosyl group from 5-phosphoribosylpyrophosphate onto the purine. Plays a central role in the generation of purine nucleotides through the purine salvage pathway.
    • Pathway

      Purine metabolism; IMP biosynthesis via salvage pathway; IMP from hypoxanthine: step 1/1.
    • Involvement in disease

      Defects in HPRT1 are the cause of Lesch-Nyhan syndrome (LNS) [MIM:300322]. LNS is characterized by complete lack of enzymatic activity that results in hyperuricemia, choreoathetosis, mental retardation, and compulsive self-mutilation.
      Defects in HPRT1 are the cause of gout HPRT-related (GOUT-HPRT) [MIM:300323]; also known as HPRT-related gout or Kelley-Seegmiller syndrome. Gout is characterized by partial enzyme activity and hyperuricemia.
    • Sequence similarities

      Belongs to the purine/pyrimidine phosphoribosyltransferase family.
    • Cellular localization

      Cytoplasm.
    • Information by UniProt
    • Database links

    • Alternative names

      • HGPRT antibody
      • HGPRTase antibody
      • HPRT 1 antibody
      • HPRT_HUMAN antibody
      • HPRT1 antibody
      • Hypoxanthine guanine phosphoribosyltransferase antibody
      • Hypoxanthine phosphoribosyltransferase 1 (Lesch Nyhan syndrome) antibody
      • Hypoxanthine phosphoribosyltransferase 1 antibody
      • Hypoxanthine-guanine phosphoribosyltransferase antibody
      see all

    References

    ab240051 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab240051.
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    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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