Product nameAnti-HPS1 antibody [EP8429(2)]
See all HPS1 primary antibodies
DescriptionRabbit monoclonal [EP8429(2)] to HPS1
Tested applicationsSuitable for: WB, ICC/IF, Flow Cyt, IHC-Pmore details
Unsuitable for: IP
Species reactivityReacts with: Mouse, Rat, Human
Synthetic peptide within Human HPS1 aa 400-500 (Cysteine residue). The exact sequence is proprietary.
Database link: Q92902
- A375, HeLa, HT-29 and K562 cell lysates; Human breast and muscle tissues; HeLa cells; K562 cells.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab174301 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/5000. Predicted molecular weight: 79 kDa.|
|ICC/IF||1/50 - 1/100.|
|Flow Cyt||1/10 - 1/100.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
|IHC-P||1/50 - 1/100.|
FunctionComponent of multiple cytoplasmic organelles. Apparently crucial for their normal development and function. May be involved in intracellular protein sorting.
Involvement in diseaseDefects in HPS1 are the cause of Hermansky-Pudlak syndrome type 1 (HPS1) [MIM:203300]. Hermansky-Pudlak syndrome (HPS) is a genetically heterogeneous, rare, autosomal recessive disorder characterized by oculocutaneous albinism, bleeding due to platelet storage pool deficiency, and lysosomal storage defects. This syndrome results from defects of diverse cytoplasmic organelles including melanosomes, platelet dense granules and lysosomes. Ceroid storage in the lungs is associated with pulmonary fibrosis, a common cause of premature death in individuals with HPS.
- Information by UniProt
- Hermansky Pudlak syndrome 1 protein antibody
- Hermansky Pudlak syndrome gene antibody
- Hermansky Pudlak syndrome type 1 antibody
All lanes : Anti-HPS1 antibody [EP8429(2)] (ab174301) at 1/1000 dilution
Lane 1 : A375 cell lysate
Lane 2 : HeLa cell lysate
Lane 3 : HT-29 cell lysate
Lane 4 : K562 cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 79 kDa
Immunofluorescent analysis of HeLa cells labeling HPS1 with ab174301 at 1/50 dilution.
Immunohistochemical analysis of paraffin-embedded Human breast tissue labeling HPS1 with ab174301 at 1/50 dilution.
Immunohistochemical analysis of paraffin-embedded Human muscle tissue labeling HPS1 with ab174301 at 1/50 dilution.
Flow cytometric analysis of permeabilized K562 cells with ab174301 at 1/10 dilution (red) compared to a rabbit IgG negative control (green).
ab174301 has not yet been referenced specifically in any publications.