Overview

  • Product name
    Anti-HPV antibody [BPV-1/1H8 + CAMVIR]
    See all HPV primary antibodies
  • Description
    Mouse monoclonal [BPV-1/1H8 + CAMVIR] to HPV
  • Host species
    Mouse
  • Tested applications
    Suitable for: IHC-P, ELISA, WBmore details
  • Immunogen

    HPV Cocktail Broad Spectrum (HPV-1, 6, 11, 16-16, 18 and 31)

  • Positive control
    • IHC-P: Infected cervical biopsy tissue. WB: Control and DCT siRNA transfected HaCaT cell lysate.
  • General notes

    Please note that this antibody is an oligoclonal antibody. It is a cocktail of monoclonal antibodies that have been carefully selected. Oligoclonal antibodies have not only the specificity and batch-to-batch consistency of a monoclonal antibody, but also have the advantage of the sensitivity of a polyclonal antibody due to their ability to recognize multiple epitopes on an antigen. This cocktail is a mix of BPV-1/1H8 (IgG) and CAMVIR (IgG2a).

    The broad spectrum HPV antibody was produced (1H8) against SDS-disrupted bovine papillomavirus type 1 (BPV-1) and used to identify the product of the L1 open reading frame (ORF) of BPV-1. IH8 was found to be reactive with purified major capsid protein (MCP). The antibody was tested with ELISA and with an immunofluorescent technique and detected HPV-1, 6, 11, 16-16, 18, and 31 in formalin-fixed paraffin-embedded biopsy specimens. The CAMVIR-1 antibody was raised against the major capsid protein L1 of human papillomavirus type 16, using a recombinant vaccinia virus that expresses the L1 protein, as a target for screening. This antibody, designated CAMVIR-1, reacted with a 56 kilodalton protein in cells infected with L1-vaccinia virus, and the protein was present in HPV16. This antibody cocktail may also work in PAP smears, but has not been tested. A panel of p16 and Ki-67 can be used for further evaluation.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    pH: 7.30
    Preservatives: Proprietary preservative, 0.09% Sodium azide
    Constituents: PBS, Tissue culture supernatant
  • Concentration information loading...
  • Primary antibody notes
    The broad spectrum HPV antibody was produced (1H8) against SDS-disrupted bovine papillomavirus type 1 (BPV-1) and used to identify the product of the L1 open reading frame (ORF) of BPV-1. IH8 was found to be reactive with purified major capsid protein (MCP). The antibody was tested with ELISA and with an immunofluorescent technique and detected HPV-1, 6, 11, 16-16, 18, and 31 in formalin-fixed paraffin-embedded biopsy specimens. The CAMVIR-1 antibody was raised against the major capsid protein L1 of human papillomavirus type 16, using a recombinant vaccinia virus that expresses the L1 protein, as a target for screening. This antibody, designated CAMVIR-1, reacted with a 56 kilodalton protein in cells infected with L1-vaccinia virus, and the protein was present in HPV16. This antibody cocktail may also work in PAP smears, but has not been tested. A panel of p16 and Ki-67 can be used for further evaluation.
  • Clonality
    Monoclonal
  • Clone number
    BPV-1/1H8 + CAMVIR
  • Myeloma
    unknown
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab2417 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/80 - 1/160. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
ELISA Use at an assay dependent dilution.
WB Use at an assay dependent concentration. PubMed: 22479413

Target

  • Relevance
    Human papillomaviruses (HPV) are small DNA viruses which infect epithelia of the skin and mucosa. Over 90 types have been identified and they mostly cause a variety of benign lesions such as warts and verrucae. However, some subtypes, notably types 16 and 18, 31 and 33, have been confirmed as agents which cause cervical cancer.
  • Cellular localization
    Nuclear
  • Alternative names
    • HPV antibody
    • Human papillomavirus antibody

Images

  • Western blot analysis of L1 and actin levels in (A) uninfected, control and DCT siRNA transfected HaCaTs.

  • ab2417 staining HPV-infected human cervix tissue sections.

References

This product has been referenced in:
See all 9 Publications for this product

Customer reviews and Q&As

1-9 of 9 Abreviews or Q&A

Answer

I recommend ab97023, goat anti-mouse IgG H&L (heavy and light chains), assuming you want an HRP conjugate (horseradish peroxidase) for visualization. We also have fluorochrome conjugates and alkaline phosphatase conjugates, among others.

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Abcam has not validated the combination of species/application used in this Abreview.
Application
Western blot
Sample
Tobacco Recombinant protein (Plant cell)
Loading amount
21 µg
Specification
Plant cell
Gel Running Conditions
Reduced Denaturing (gel 12%)
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 4°C

Mr. Diego Grando Módolo

Verified customer

Submitted Dec 18 2012

Answer

Thank you for contacting Abcam

Theprotein carrier forHPV Cocktail Broad Spectrumis heat attenuated goat serum.

Please let me know if there is anything else I can help you with.

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Answer

Thank you for your enquiry and for your patience. It has taken some time to gather the information.

Regardnig ab2417 Anti-HPV antibody [BPV-1/1H8 + CAMVIR], I can confirm thiswas raised against the major capsid protein L1. I have copied below some further information:

The broad spectrum HPV antibody was produced (1H8) against SDS-disrupted bovine papillomavirus type 1 (BPV-1) and used to identify the product of the L1 open reading frame (ORF) of BPV-1. IH8 was found to be reactive with purified major capsid protein (MCP). The antibody was tested with ELISA and with an immunofluorescent technique and detected HPV-1, 6, 11, 16-16, 18, and 31 in formalin-fixed paraffin-embedded biopsy specimens. The CAMVIR-1 antibody was raised against the major capsid protein L1 of human papillomavirus type 16, using a recombinant vaccinia virus that expresses the L1 protein, as a target for screening. This antibody, designated CAMVIR-1, reacted with a 56 kilodalton protein in cells infected with L1-vaccinia virus, and the protein was present in HPV16. This antibody cocktail may also work in PAP smears, but has not been tested. A panel of p16 and Ki-67 can be used for further evaluation.

Regarding ab30908HPV16 L1 antibody [289-16981], this should detectL1 as it was raised against HPV Type 16L1 protein.

The ab75574 Anti-HPV antibody [K1H8],hasbeen sourced and tested externally. I am sorry we have not been able to obtain any further information yet for these antibodies, and therefore I would assume this has regrettably not been specifically tested for detection of L1.

For western blotting, I can confirm that all tested applications covered by our guarantee are listed on the online datasheets which are updated as soon as we receive further information. I can confirm that ab2417 and ab30908 have been tested in WB. The ab75574 has not been tested in WB.

I hope this information will be helpful to you. If you have any further questions, please do not hesitate to contact us.

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Question
Answer

Thank you for contacting us.

My colleague has created an SDS for ab2417 and it is now available for download from our website. I have also attached it to this email. Please let me know if you need any additional information.

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Question
Answer

Thanks you for your enquiry. Based on the following publications, there are cases where the HPV expression was found in the cytoplasm and nuclear region. 1) CASE REPORT: Corneal papilloma associated with papillomavirus in a one-humped camel (Camelus dromedarius). Veterinary Ophthalmology. Volume 13, Issue Supplement s1, pages 100–102, September 2010. (Fig 3) http://onlinelibrary.wiley.com/doi/10.1111/j.1463-5224.2010.00795.x/full 2) Clinical, pathological and immunohistochemical findings of bovine cutaneous papillomatosis. Ankara Üniv Vet Fak Derg, 58, 161-165, 2011 (Fig 2) http://dergiler.ankara.edu.tr/dergiler/11/1565/16994.pdf Therefore, the results you are seeing is not uncommon. Although on our datasheet it does state nuclear, this is information from SwissProt and may not be the case in an actual experiment. I hope this information will be helpful.

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Answer

Thank you for your enquiry. This antibody was used at a 1:80 dilution and the secondary antibody was a MACH3 Mouse AP Polymer. Please bear in mind that different samples and buffers used may require some optimization. I hope this information will be helpful. If there is anything else that I can help you with please do not hesitate to contact me. Have a nice day!

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Answer

The antibody was used at a concentration of 1:80 in IHC-P. Secondary was AP-polymer. We have not carried out ICC/IF for this antibody. Thus, we are unable to guarantee the suitability of this application. Probably antibody could be further diluted.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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