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I'm interested inantibody "ab30908".
And I want to knowthis antibody can cross react withL1 protein of another type of HPV.
If you don't have any information about cross reactivity,can you give mesome information about the sequence of immunogen?
And I also want to know "ab2417" and "ab75574" can react withL1 protein of HPV.
Can I use these antibodies in WB?
Asked on May 23 2012
Thank you for your enquiry and for your patience. It has taken some time to gather the information.
Regardnig ab2417 Anti-HPV antibody [BPV-1/1H8 + CAMVIR], I can confirm thiswas raised against the major capsid protein L1. I have copied below some further information:
The broad spectrum HPV antibody was produced (1H8) against SDS-disrupted bovine papillomavirus type 1 (BPV-1) and used to identify the product of the L1 open reading frame (ORF) of BPV-1. IH8 was found to be reactive with purified major capsid protein (MCP). The antibody was tested with ELISA and with an immunofluorescent technique and detected HPV-1, 6, 11, 16-16, 18, and 31 in formalin-fixed paraffin-embedded biopsy specimens. The CAMVIR-1 antibody was raised against the major capsid protein L1 of human papillomavirus type 16, using a recombinant vaccinia virus that expresses the L1 protein, as a target for screening. This antibody, designated CAMVIR-1, reacted with a 56 kilodalton protein in cells infected with L1-vaccinia virus, and the protein was present in HPV16. This antibody cocktail may also work in PAP smears, but has not been tested. A panel of p16 and Ki-67 can be used for further evaluation.
Regarding ab30908HPV16 L1 antibody [289-16981], this should detectL1 as it was raised against HPV Type 16L1 protein.
The ab75574 Anti-HPV antibody [K1H8],hasbeen sourced and tested externally. I am sorry we have not been able to obtain any further information yet for these antibodies, and therefore I would assume this has regrettably not been specifically tested for detection of L1.
For western blotting, I can confirm that all tested applications covered by our guarantee are listed on the online datasheets which are updated as soon as we receive further information. I can confirm that ab2417 and ab30908 have been tested in WB. The ab75574 has not been tested in WB.
I hope this information will be helpful to you. If you have any further questions, please do not hesitate to contact us.
Answered on May 23 2012