Key features and details
- Mouse monoclonal [10E12] to HPV11 E2
- Suitable for: WB, ELISA, ICC/IF
- Reacts with: Human papillomavirus
- Isotype: IgG1
Product nameAnti-HPV11 E2 antibody [10E12]
DescriptionMouse monoclonal [10E12] to HPV11 E2
SpecificityReacts in WB, and ELISA with HPV11 native E2 protein as well as recombinant E2 protein expressed in bacteria and Cos 7 cells.
Tested applicationsSuitable for: WB, ELISA, ICC/IFmore details
Unsuitable for: IP
Species reactivityReacts with: Human papillomavirus
Recombinant full length HPV11 E2 protein purified from bacteria.
EpitopeMapped to amino acids 202-284
- U20S or Hela cells and lysates
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Store in the dark. Avoid repeated freeze / thaw cycles.
Storage bufferpH: 7.40
Preservative: 0.1% Sodium azide
Concentration information loading...
PurityProtein G purified
Purification notesPurified from bacteria by affinity chromatography.
Our Abpromise guarantee covers the use of ab100968 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000. Predicted molecular weight: 42 kDa.|
|ICC/IF||Use at an assay dependent dilution.|
RelevanceHuman papillomaviruses (HPV) are small DNA viruses which infect epithelia of the skin and mucosa. Over 90 types have been identified and they mostly cause a variety of benign lesions such as warts and verrucae. However, some subtypes, notably types 16 and 18, 31 and 33, have been confirmed as agents which cause cervical cancer. Human Papillomavirus (HPV) E2 proteins are the major viral regulators of transcription and replication during the viral life cycle.
Cellular localizationHost nucleus
- SwissProt: P04015 Human papillomavirus
- Human papillomaviruses 11 E2 antibody
All lanes : Anti-HPV11 E2 antibody [10E12] (ab100968) at 1/1000 dilution
Lanes 1 & 4 : lysate of U20S cells transfected with plasmid expressing
HPV11 E2 protein
Lane 2 : lysate of plain U20S cells
Lane 3 : lysate of plain HeLa cells
Lane 5 : marker
Predicted band size: 42 kDa
Immunofluorescence (IF) analysis of HPV11 E2 protein in U2OS cells.
IF was carried out with U2OS cell transfected with plasmid expressing HPV11 E2 protein. All the nuclei were stained by DAPI. Green color represents nucleus localized HPV11 E2 detected by ab100968. IF analysis of E2 protein shows similar result also in HeLa cells.
ab100968 has been referenced in 2 publications.
- Nanbo A et al. Epstein-Barr virus exploits host endocytic machinery for cell-to-cell viral transmission rather than a virological synapse. J Gen Virol 97:2989-3006 (2016). PubMed: 27655016
- Kivipõld P et al. DAXX modulates human papillomavirus early gene expression and genome replication in U2OS cells. Virol J 12:104 (2015). IF . PubMed: 26148509