Key features and details
- Mouse monoclonal [9H5] to HPV11 E7
- Suitable for: WB, IP, ELISA, ICC/IF
- Reacts with: Human papillomavirus
- Isotype: IgG2a
Product nameAnti-HPV11 E7 antibody [9H5]
DescriptionMouse monoclonal [9H5] to HPV11 E7
SpecificityReacts in WB, IP, ELISA with HPV11 native E7 protein as well as recombinant E7 protein expressed in bacteria and Cos7 cells.
Tested applicationsSuitable for: WB, IP, ELISA, ICC/IFmore details
Species reactivityReacts with: Human papillomavirus
Recombinant full length HPV11 E7 protein purified from bacteria.
EpitopeMapped to amino acids 36-70
- WB: U2OS and HeLa cell lysates ICC/IF: U2OS cells transfected with plasmid expressing HPV-11 E7 protein IP: lysate of U20S cells
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage bufferpH: 7.40
Preservative: 0.1% Sodium azide
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab100967 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000. Predicted molecular weight: 11 kDa.|
|ICC/IF||Use at an assay dependent dilution.|
RelevanceInfections by human papillomaviruses (HPV) can cause relatively harmless plantar warts and also more serious genital warts that can increase the risk to women for developing cervical cancer. Infections with HPV11 triggers genital warts. E7 is a protein that is encoded by by HPV11 and is implicated in the disruption of host cells and promoting their proliferation.
- SwissProt: P04020 Human papillomavirus
- Human papillomavirus E7 protein antibody
All lanes : Anti-HPV11 E7 antibody [9H5] (ab100967) at 1/1000 dilution
Lanes 1 & 4 : lysate of U20S cells transfected with plasmid expressing HPV11 E7 protein
Lane 2 : lysate of plain U20S cells
Lane 3 : lysate of HeLa cells
Lane 5 : marker
Predicted band size: 11 kDa
Immunoprecipitation (IP) analysis of HPV11 E7 protein.
Lane 1 represents the marker.
Lane 2 shows detection of E7 protein by ab100967 in lysate of U20S cells.
In lane 3 the lower band is precipitated E7, upper bands are light and heavy chain of the antibody.
Lane 4 is precipitation without ab100967
Lane 5 shows IP experiment with plain U2OS cells.
Immunofluorescence (IF) analysis of HPV11 E7 protein in U2OS cells.
IF was carried out using U2OS cells transfected with plasmid expressing HPV11 E7 protein. All the nuclei were stained by DAPI. Green color represents nucleus localized HPV11 E7 detected by ab100967. IF analysis of E7 protein shows similar result also in HeLa cells.
ab100967 has not yet been referenced specifically in any publications.