Overview

  • Product name

    Anti-HPV16 E2 antibody [TVG 261]
    See all HPV16 E2 primary antibodies
  • Description

    Mouse monoclonal [TVG 261] to HPV16 E2
  • Host species

    Mouse
  • Tested applications

    Suitable for: ELISA, WBmore details
  • Species reactivity

    Reacts with: Other species
  • Immunogen

    Vaccinia-E2 followed by intravenous injection of the maltose binding protein MBP-E2 (generated from an expression vector in which the E2 ORF was cloned in frame with malE).

  • Epitope

    aa2-17 - ETLCQRLNVCQDKILT.
  • General notes


    The human papilloma virus (HPV) family of DNA tumor viruses includes HPV-16 and HPV-18, which are associated with a large proportion of cervical cancer cases. E1 and E2 are proteins involved in the regulation of viral DNA replication. TVG 261 is useful for detection of protein in cervical lesions.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    Constituent: PBS
  • Concentration information loading...
  • Purity

    Protein A/G purified
  • Primary antibody notes

    The human papilloma virus (HPV) family of DNA tumor viruses includes HPV-16 and HPV-18, which are associated with a large proportion of cervical cancer cases. E1 and E2 are proteins involved in the regulation of viral DNA replication. TVG 261 is useful for detection of protein in cervical lesions.
  • Clonality

    Monoclonal
  • Clone number

    TVG 261
  • Myeloma

    NS0/1
  • Isotype

    IgG1
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab17185 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA Use at an assay dependent dilution.
WB Use at an assay dependent dilution. Predicted molecular weight: 42 kDa.

Target

  • Alternative names

    • HPV16 regulatory protein E2 antibody
    • Human papilloma virus 16 E2 antibody
    • Human papilloma virus 16 regulatory protein E2 antibody
    • Human papillomavirus type 16 E2 antibody
    • Human papillomavirus type 16 regulatory protein E2 antibody
    • Regulatory protein E2 antibody
    see all

References

This product has been referenced in:

  • Evans MR  et al. Human Papillomavirus 16 E2 Regulates Keratinocyte Gene Expression Relevant to Cancer and the Viral Life Cycle. J Virol 93:N/A (2019). Read more (PubMed: 30518656) »
  • Ludwig S  et al. Molecular and Functional Profiles of Exosomes From HPV(+) and HPV(-) Head and Neck Cancer Cell Lines. Front Oncol 8:445 (2018). Read more (PubMed: 30370252) »
See all 10 Publications for this product

Customer reviews and Q&As

1-3 of 3 Abreviews or Q&A

Answer

Thank you for contacting Abcam. I have attached the MSDS for all except ab500, ChIP kit. I am still waiting for the laboratory to get back to me on that one and I will send it on as soon as I get it. If there is anything else I can do, please let me know.

Read More
Application
Western blot
Sample
Human Cell lysate - whole cell (293T)
Loading amount
300000 cells
Specification
293T
Gel Running Conditions
Reduced Denaturing
Blocking step
Milk as blocking agent for 16 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C

Abcam user community

Verified customer

Submitted Aug 12 2010

Application
Western blot
Sample
African Green Monkey Cell lysate - whole cell (Cos)
Loading amount
500000 cells
Specification
Cos
Treatment
Transfected with MV11.HPV16E2
Gel Running Conditions
Reduced Denaturing (12%)
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 4°C

Dr. Clare Davy

Verified customer

Submitted Sep 02 2008

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