Anti-HPV16 E6 + HPV18 E6 antibody [C1P5] (ab70)

Mouse monoclonal HPV16 E6 + HPV18 E6 antibody [C1P5]. Validated in WB, IP, IHC and tested in Human papillomavirus. Cited in 16 publication(s). Independently reviewed in 2 review(s).

Overview

  • Product name

    Anti-HPV16 E6 + HPV18 E6 antibody [C1P5]
    See all HPV16 E6 + HPV18 E6 primary antibodies
  • Description

    Mouse monoclonal [C1P5] to HPV16 E6 + HPV18 E6
  • Host species

    Mouse
  • Tested applications

    Suitable for: WB, IP, IHC-Pmore details
  • Species reactivity

    Reacts with: Human papillomavirus
  • Immunogen

    Gel-purified HPV-18 E6-beta galactosidase fusion protein.

  • Positive control

    • Human cervical cancer cells.
  • General notes

    Clinical relevance: Detection of HPV in cervical smears and biopsies. Uses: Analysis of E6 expression in cell transformation studies.

    The E6 proteins are known to form oligomers; PMID19917295, PMID17209544.

Properties

Applications

Our Abpromise guarantee covers the use of ab70 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration. Detects a band of approximately 16.5 kDa.

16.5 kDa band is HPV-18, 17 kDa band is HPV-16.

The E6 proteins are known to form oligomers; PMID19917295, PMID17209544.

IP Use at an assay dependent concentration.
IHC-P Use at an assay dependent concentration.

Target

  • Relevance

    Human Papilloma virus (HPV) is responsible for the development of type and tissue specific papilloma in the oral cavity and in the larynx. In the skin papilloma virus can cause different types of warts and in the ano-genital region it is associated with condyloma or carcinoma. HPV types 1,6 and 11 usually are associated with benign transformations of the tissues, while e.g. HPV types 16, 18 and 31 seem to be responsible for the development of some carcinoma like cervical carcinoma. The role of HPV in non-melanoic tumours of the skin is under discussion. Protein E6 is a Transcriptional transactivator, binding double stranded DNA. It has transforming activity inactivating, with E6-AP ubiquitin-protein ligase, the human TP53/p53 tumor suppressor protein by targeting it for degradation.
  • Database links

    • Alternative names

      • E6 antibody
      • Human Papilloma Virus antibody
      • Human papillomavirus type 16 E6 antibody
      • Human papillomavirus type 18 E6 antibody
      • Protein E6 antibody
      see all

    Images

    • Formalin-fixed, paraffin-embedded human uterine cervix squamous cell carcinoma tissue stained for HPV16 E6 + HPV18 E6 using ab70 at 1/200 dilution in immunohistochemical analysis.

      Incubated with primary antibody for 1 hour at 25°C.

      Heat mediated antigen retrieval at pH 6.

      See Abreview

    • Subconfluent proliferating MCF-7 or T47D cells were transiently transfected overnight with empty pcDNA3 vector, with FLAG wild-type E6 or zinc finger defective mutant E6 (E6-(C66,136G)). The cells were washed, post-incubated for 24 hours to allow gene expression, and harvested for Western blotting to detect the E6 oncoprotein using ab70.

    References

    This product has been referenced in:

    • Jiang Z  et al. Monoclonal Antibodies Against Human Papillomavirus E6 and E7 Oncoproteins Inhibit Tumor Growth in Experimental Cervical Cancer. Transl Oncol 12:1289-1295 (2019). Read more (PubMed: 31325765) »
    • Wang X  et al. Human papillomavirus type 16 E6 oncoprotein promotes proliferation and invasion of non-small cell lung cancer cells through Toll-like receptor 3 signaling pathway. J Med Virol N/A:N/A (2017). WB ; Human . Read more (PubMed: 28480962) »
    See all 18 Publications for this product

    Customer reviews and Q&As

    Filter by Application

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    1-2 of 2 Abreviews

    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Human Tissue sections (Uterine cervix, Squamous cell carcinoma)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: pH 6.0
    Permeabilization
    No
    Specification
    Uterine cervix, Squamous cell carcinoma
    Fixative
    Formaldehyde

    Mr. Allan Wang

    Verified customer

    Submitted Apr 17 2018

    Application
    Immunocytochemistry
    Sample
    Cow Cultured Cells (Epithelial cells of cows with BPV and cells free-B)
    Specification
    Epithelial cells of cows with BPV and cells free-B
    Fixative
    Paraformaldehyde
    Permeabilization
    Yes - Tritonx100 (0,1%),
    Blocking step
    BSA as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C

    Abcam user community

    Verified customer

    Submitted Aug 31 2012

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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