Anti-HPV16 E6 + HPV18 E6 antibody [C1P5] (ab70)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HPV16 E6 + HPV18 E6 antibody [C1P5] (ab70)](https://www.abcam.com/ps/products/0/ab70/Images/ab70-334509-anti-hpv16-e6-hpv18-e6-antibody-c1p5-immunohistochemistry.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HPV16 E6 + HPV18 E6 antibody [C1P5] (ab70)")
Key features and details
- Mouse monoclonal [C1P5] to HPV16 E6 + HPV18 E6
- Suitable for: WB, IP, IHC-P
- Reacts with: Human papillomavirus
- Isotype: IgG1
Overview
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Product name
Anti-HPV16 E6 + HPV18 E6 antibody [C1P5]
See all HPV16 E6 + HPV18 E6 primary antibodies -
Description
Mouse monoclonal [C1P5] to HPV16 E6 + HPV18 E6 -
Host species
Mouse -
Tested applications
Suitable for: WB, IP, IHC-Pmore details -
Species reactivity
Reacts with: Human papillomavirus -
Immunogen
Gel-purified HPV-18 E6-beta galactosidase fusion protein.
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Positive control
- Human cervical cancer cells.
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General notes
Clinical relevance: Detection of HPV in cervical smears and biopsies. Uses: Analysis of E6 expression in cell transformation studies.
The E6 proteins are known to form oligomers; PMID19917295, PMID17209544.
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Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
C1P5 -
Myeloma
NS1 -
Isotype
IgG1 -
Light chain type
unknown -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab70 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
Use at an assay dependent concentration. Detects a band of approximately 16.5 kDa.
16.5 kDa band is HPV-18, 17 kDa band is HPV-16. The E6 proteins are known to form oligomers; PMID19917295, PMID17209544. |
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| IP |
Use at an assay dependent concentration.
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| IHC-P | (1) |
Use at an assay dependent concentration.
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| Notes |
|---|
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WB
Use at an assay dependent concentration. Detects a band of approximately 16.5 kDa. 16.5 kDa band is HPV-18, 17 kDa band is HPV-16. The E6 proteins are known to form oligomers; PMID19917295, PMID17209544. |
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IP
Use at an assay dependent concentration. |
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IHC-P
Use at an assay dependent concentration. |
Target
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Relevance
Human Papilloma virus (HPV) is responsible for the development of type and tissue specific papilloma in the oral cavity and in the larynx. In the skin papilloma virus can cause different types of warts and in the ano-genital region it is associated with condyloma or carcinoma. HPV types 1,6 and 11 usually are associated with benign transformations of the tissues, while e.g. HPV types 16, 18 and 31 seem to be responsible for the development of some carcinoma like cervical carcinoma. The role of HPV in non-melanoic tumours of the skin is under discussion. Protein E6 is a Transcriptional transactivator, binding double stranded DNA. It has transforming activity inactivating, with E6-AP ubiquitin-protein ligase, the human TP53/p53 tumor suppressor protein by targeting it for degradation. -
Database links
- Entrez Gene: 1489078 Human papillomavirus
- SwissProt: P03126 Human papillomavirus
- SwissProt: P06463 Human papillomavirus
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Alternative names
- E6 antibody
- Human Papilloma Virus antibody
- Human papillomavirus type 16 E6 antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HPV16 E6 + HPV18 E6 antibody [C1P5] (ab70)Image courtesy of Mr Allan Wang
Formalin-fixed, paraffin-embedded human uterine cervix squamous cell carcinoma tissue stained for HPV16 E6 + HPV18 E6 using ab70 at 1/200 dilution in immunohistochemical analysis.
Incubated with primary antibody for 1 hour at 25°C.
Heat mediated antigen retrieval at pH 6.
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![Western blot - Anti-HPV16 E6 + HPV18 E6 antibody [C1P5] (ab70)](https://www.abcam.com/ps/products/0/ab70/Images/ab70-3282-anti-hpv16-e6-hpv18-e6-antibody-c1p5-western-blot.jpg)
Western blot - Anti-HPV16 E6 + HPV18 E6 antibody [C1P5] (ab70)Image from Zhang Y et al, J Biol Chem. 2005 Sep 30;280(39):33165-77. Epub 2005 Jun 27, Fig 9. DOI 10.1074/jbc.M505124200Subconfluent proliferating MCF-7 or T47D cells were transiently transfected overnight with empty pcDNA3 vector, with FLAG wild-type E6 or zinc finger defective mutant E6 (E6-(C66,136G)). The cells were washed, post-incubated for 24 hours to allow gene expression, and harvested for Western blotting to detect the E6 oncoprotein using ab70.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (30)
ab70 has been referenced in 30 publications.
- Chen Y et al. In vitro and in vivo growth inhibition of human cervical cancer cells via human papillomavirus E6/E7 mRNAs' cleavage by CRISPR/Cas13a system. Antiviral Res 178:104794 (2020). PubMed: 32298665
- Li C et al. Reprogrammed CRISPR-Cas13a targeting the HPV16/18 E6 gene inhibits proliferation and induces apoptosis in E6-transformed keratinocytes. Exp Ther Med 19:3856-3860 (2020). PubMed: 32346451
- Zhang C et al. DNMT1 Enhances the Radiosensitivity of HPV-Positive Head and Neck Squamous Cell Carcinomas via Downregulating SMG1. Onco Targets Ther 13:4201-4211 (2020). PubMed: 32523356
- Wang F et al. BCCIPß facilitates p53 ubiquitination via binding with E6 protein in high-risk HPV positive head and neck squamous cell carcinoma. Biochem Biophys Res Commun 529:685-691 (2020). PubMed: 32736693
- Ding X et al. A DHX9-lncRNA-MDM2 interaction regulates cell invasion and angiogenesis of cervical cancer. Cell Death Differ 26:1750-1765 (2019). PubMed: 30518908