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  1. Link

    hpv16-l1-antibody-camvir-1-ab69.pdf

  1. Send me a copy of this email
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Microbiology Organism Virus DNA Virus double stranded DNA Virus Human Papillomavirus
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Anti-HPV16 L1 antibody [CamVir 1] (ab69)

  • Datasheet
  • SDS
Submit a review Q&A (13)References (20)

Product price, shipping and contact information

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Promotion Information

Abpromise

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Find out more.

Key features and details

  • Mouse monoclonal [CamVir 1] to HPV16 L1
  • Suitable for: WB, IP, IHC-P
  • Reacts with: Human papillomavirus
  • Isotype: IgG2a

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Protein
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Recombinant HPV16 L1 protein (ab119880)
Protein
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Recombinant HPV18 L1 protein (ab119881)
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Overview

  • Product name

    Anti-HPV16 L1 antibody [CamVir 1]
    See all HPV16 L1 primary antibodies
  • Description

    Mouse monoclonal [CamVir 1] to HPV16 L1
  • Host species

    Mouse
  • Specificity

    The antibody reacts with a 56 kDa protein in cells infected with L1-vaccinia virus, the protein being present in a predominantly nuclear location. The antibody reacts very strongly with biopsy specimins containing HPV-16 or -33; very weak reactions were occasionally observed with biopsy specimens or smears containing HPV-6 or HPV-11. Cross-reacts with HPV37.
  • Tested applications

    Suitable for: WB, IP, IHC-Pmore details
  • Species reactivity

    Reacts with: Human papillomavirus
  • Immunogen

    Fusion protein corresponding to Human papillomavirus HPV16 L1. A b galactosidase-L1 fusion protein purified by PAGE.

  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    Phosphate buffered saline
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    CamVir 1
  • Myeloma

    NS0
  • Isotype

    IgG2a
  • Light chain type

    unknown
  • Research areas

    • Microbiology
    • Organism
    • Virus
    • DNA Virus
    • double stranded DNA Virus
    • Human Papillomavirus
    • Cancer
    • Oncoproteins/suppressors
    • Viral proteins
    • HPV
    • Microbiology
    • Protein
    • Viral Protein

Associated products

  • Compatible Secondaries

    • Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)
    • Goat Anti-Mouse IgG H&L (HRP) (ab205719)
  • Isotype control

    • Mouse IgG2a, kappa monoclonal [MG2a-53] - Isotype control (ab18415)
  • Recombinant Protein

    • Recombinant HPV16 L1 protein (ab119880)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab69 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
Use at an assay dependent concentration. Predicted molecular weight: 56 kDa.
IP
Use at an assay dependent concentration.
IHC-P
Use at an assay dependent concentration.
Notes
WB
Use at an assay dependent concentration. Predicted molecular weight: 56 kDa.
IP
Use at an assay dependent concentration.
IHC-P
Use at an assay dependent concentration.

Target

  • Relevance

    L1 is a major capsid protein of type 16 human papilloma virus. Infection with specific types of HPV has been associated with an increased risk of developing cervical neoplasia. HPV types 6 and 11 have been associated with relatively benign diseases such as genital warts but types 16 and 18 are strongly associated with cervical, vaginal, and vulvar malignancies.
  • Cellular localization

    Virion. Host Nucleus.
  • Database links

    • Entrez Gene: 1489082 Human papillomavirus
    • SwissProt: P03101 Human papillomavirus
    • Alternative names

      • HPV-16 antibody
      • HPV-16 capsid antibody
      • HPV16 capsid protein antibody
      • HPV16 L1 antibody
      • HPV16 major capsid protein L1 antibody
      • Human papillomavirus type 16 L1 antibody
      • Human papillomavirus type 16 major capsid protein L1 antibody
      • L1 antibody
      • Major capsid L1 protein antibody
      • Major capsid protein antibody
      • Major capsid protein L1 antibody
      see all

    Protocols

    • Immunoprecipitation protocols
    • Immunohistochemistry protocols
    • Western blot protocols

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (20)

    Publishing research using ab69? Please let us know so that we can cite the reference in this datasheet.

    ab69 has been referenced in 20 publications.

    • Karimi H  et al. Combination therapy using human papillomavirus L1/E6/E7 genes and archaeosome: a nanovaccine confer immuneadjuvanting effects to fight cervical cancer. Sci Rep 10:5787 (2020). PubMed: 32238821
    • Mboumba Bouassa RS  et al. Serum and cervicovaginal IgG immune responses against a7 and a9 HPV in non-vaccinated women at risk for cervical cancer: Implication for catch-up prophylactic HPV vaccination. PLoS One 15:e0233084 (2020). PubMed: 32421735
    • Ðuraševic S  et al. The Effects of Meldonium on the Renal Acute Ischemia/Reperfusion Injury in Rats. Int J Mol Sci 20:N/A (2019). PubMed: 31731785
    • Wu X  et al. Induction of neutralizing antibodies by human papillomavirus vaccine generated in mammalian cells. Antib Ther 2:45-53 (2019). PubMed: 33928221
    • Xv Z  et al. Effective Neutralizing Antibody Produced in Mice Directly Immunized with Integrated Pichia pastoris Expressing HPV16L1 Protein. Viral Immunol 32:308-317 (2019). PubMed: 31381497
    View all Publications for this product

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    1-10 of 13 Abreviews or Q&A

    Question

    Thank you very much for the information.
    I am however looking for the binding site of your camVir1 antibody. That is against HPV16 L1. Do you have a paper on that?
    Thanking you for your help

    Read More

    Abcam community

    Verified customer

    Asked on Feb 21 2013

    Answer

    Thank you for contacting us.

    Unfortunately, we do not have any other information about the binding site, I am afraid!. The CamVir1 clone is a published clone, please check the literature if anybody else have published any more details about the epitope.

    I hope this information is nevertheless helpful to you. Please do not hesitate to contact us if you need any more advice or information.

    Read More

    Abcam Scientific Support

    Answered on Feb 21 2013

    Question

    Product code: 69
    Inquiry: Hi, Which epitope on L1 does camvir1 bind to? Thanks Inga

    Read More

    Abcam community

    Verified customer

    Asked on Feb 21 2013

    Answer

    Thank you for contacting us.

    The exact epitope sequence has been mapped. The immunogen is a gel-purified HPV-18 E6-b galactosidase fusion protein.

    Please check the link below of original publication;

    http://vir.sgmjournals.org/content/68/5/1351.full.pdf

    I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

    Read More

    Abcam Scientific Support

    Answered on Feb 21 2013

    Question

    What is the epitope of antibody ab69?

    Read More

    Abcam community

    Verified customer

    Asked on Sep 12 2012

    Answer

    Thank you for contacting us.

    Please find attached the requested paper.

    I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

    Use our products? Submit an Abreview. Earn rewards!
    https://www.abcam.com/abreviews

    Read More

    Abcam Scientific Support

    Answered on Sep 12 2012

    Question

    Dear technical team,

    Our customer is going to purchase ab69 and she would like to know if it has cross-reactivity to other HPV types except type 16.
    Please let me know.

    Best regards

    Read More

    Abcam community

    Verified customer

    Asked on Aug 17 2012

    Answer

    Thank you for contacting us.

    The anti-HPV16 L1 antibody [CamVir 1] (ab69) has shown some cross reactivity with other HPV types other than 16.The antibody reacts very strongly with biopsy specimens containing HPV-16 or -33, whilstvery weak reactionshave occasionally observed with biopsy specimens or smears containing HPV-6 or HPV-11. There has also been cross reactivity observed with HPV37.

    I hope this information has been of help. If you have any other questions, please do not hesitate to ask.

    Read More

    Abcam Scientific Support

    Answered on Aug 17 2012

    Question

    Hola de nuevo Abcam:
    Uno de nuestros clientes adquirió estos 3 anticuerpos con nosotros, ellos los alicuotaron y almacenaron a -20°C, ahora que tratan de usarlos ninguno de los tres funciona, nos podrán ayudar, aún están dentro de los 6 meses de abpromise?
    AB10419 LOTE XXXXXXXX
    AB69 LOTE XXXXXXXXXX
    AB10505 LOTE XXXXXXXXX
    Muchas Gracias.¡

    Read More

    Abcam community

    Verified customer

    Asked on Mar 27 2012

    Answer

    Gracias por contactarnos de nuevo.

    ¿Podrías por favor indicarme el número de pedido en el que se enviaron los anticuerpos? ¿En qué estado se recibieron? ¿Nada más recibirlos se alicuotaron y guardaron a -20C?

    ¿En qué aplicación y especies se están usando?

    Te agradezco que nos facilites estos detalles para poder entender mejor qué ha podido ocurrir para que ninguno de los tres anticuerpos funcione.

    Gracias por la colaboración. Quedo a la espera de tu respuesta.

    Read More

    Abcam Scientific Support

    Answered on Mar 27 2012

    Question

    Thank you what is the status of our order?

    Read More

    Abcam community

    Verified customer

    Asked on Jan 26 2012

    Answer

    Unfortunately shipment of this order is being held up by our accounts team.

    Our accounting department can be contacted by email at mailto:us.credits@abcam.com or by telephone using the information at the Contact Us link in the top right corner of our website. Please refer to theorder reference numberin any correspondence with our accounting department.

    I hope this is helpful. Please contact me again with any technical questions you may have.

    Read More

    Abcam Scientific Support

    Answered on Jan 26 2012

    Question

    I purchased your mouse anti-HPV 16 L1 ab a while back. I am using the ab for ELISA purposes (to test levels of HPV specific IgG). I am trying to figure out appropriate standards for my ELISA. At first, I was thinking that I might coat a plate with anti-mouse IgG, titrate purified normal mouse IgG, and probe with a goat anti-mouse IgG-HRP Ab. But I wonder, if I coat with anti-mouse IgG, could I titrate with the Ab69 and pick up a signal after probing with goat anti-mouse IgG-HRP. I'm thinking the answer is no, but wanted to check. Could you help me figure out appropriate standards for using this antibody in ELISA format?

    Read More

    Abcam community

    Verified customer

    Asked on Jan 19 2012

    Answer

    Thank you for contacting us.


    If I understand you correctly. The anti-mouse IgG would recognize your purified normal mouse IgG or it would recognize Ab69. These, in turn, would be recognized by goat anti-mouse HRP. However if you are to use ab69 against HPV in mouse tissues your detection antibody (goat anti-mouse IgG-HRP) should IgG2a specific,as it may recognize both ab69 and the Fc receptors in the tissue thereby creating a false positive.

    It seems that youare proposing a sandwich ELISA. These can be difficult to optimize. The antibodies must be detecting different epitopes of the antigen so they do not inferfere with the other antibody binding. We are unable to guarantee our antibodies in sandwich ELISA unless they have been specifically tested in that application.

    If ab69 is your detection


    I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

    Read More

    Abcam Scientific Support

    Answered on Jan 19 2012

    Question

    To Whom It May Concern:   I am requesting an MSDS (Material Safety Data Sheet) for a product used by a client of mine, Thunder Bay Regional Research.  The products are as follows:   -HPV16 L1 (product code: ab69) -HPV16 E2 (product code: ab17185) -mouse monoclonal to CD11C (BU15) (product code: ab11029) -Carbonic anhydrase 9 (product code: AB47011) -ChIP Kit (product code: AB500) -mouse monoclonal to HPV16 E6 and HPV 18 E6 (C1P5) (product code: ab70)   Are these products exempt from having an msds?  

    Read More

    Abcam community

    Verified customer

    Asked on Oct 19 2011

    Answer

    Thank you for contacting Abcam. I have attached the MSDS for all except ab500, ChIP kit. I am still waiting for the laboratory to get back to me on that one and I will send it on as soon as I get it. If there is anything else I can do, please let me know.

    Read More

    Abcam Scientific Support

    Answered on Oct 19 2011

    Question

    Dear technical team,   Our customer would like to know more information about immunogen of ab69 and ab30908.   If you have information of amino acid sequence, please let me know.   Thank you in advance.

    Read More

    Abcam community

    Verified customer

    Asked on Sep 30 2011

    Answer

    Thank you for your inquiry. Both Anti-HPV16 L1 recognise HPV Type 16, L1 (major capsid protein). Unfortunately, we do not currently have more information about the epitopes for those monoclonal antibodies. We will update the on-line datasheet for this product as soon as we receive additional data. We apologize for any inconvenience this may cause.

    Read More

    Abcam Scientific Support

    Answered on Sep 30 2011

    Question

    We routinely buy antibodies from Abcam, so I was wondering if you could send us a small amount of the ab69 HPV16-L1 (CamVir 1) antibody to test before we purchase a larger amount.

    Read More

    Abcam community

    Verified customer

    Asked on Jul 06 2005

    Answer

    Thank you for your email. We do not routinely offer free or trial sized samples for testing purposes. Our policy at Abcam is that if an antibody does not work as specified on the datasheet, we will offer a replacement or reimbursement. Should you decide to test an antibody in an application for which we do not have any information, please let us know how you get on and in return we will award you 100 points with the Abcam Loyalty Scheme which can be redeemed on a number of rewards. If you have any additional questions, please contact us again.

    Read More

    Abcam Scientific Support

    Answered on Jul 06 2005

    1-10 of 13 Abreviews or Q&A

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