Anti-HPV16 L1 antibody [CamVir 1] (ab69)

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Unfortunately, we do not have any other information about the binding site, I am afraid!. The CamVir1 clone is a published clone, please check the literature if anybody else have published any more details about the epitope.

I hope this information is nevertheless helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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The exact epitope sequence has been mapped. The immunogen is a gel-purified HPV-18 E6-b galactosidase fusion protein.

Please check the link below of original publication;

http://vir.sgmjournals.org/content/68/5/1351.full.pdf

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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Please find attached the requested paper.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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The anti-HPV16 L1 antibody [CamVir 1] (ab69) has shown some cross reactivity with other HPV types other than 16.The antibody reacts very strongly with biopsy specimens containing HPV-16 or -33, whilstvery weak reactionshave occasionally observed with biopsy specimens or smears containing HPV-6 or HPV-11. There has also been cross reactivity observed with HPV37.

I hope this information has been of help. If you have any other questions, please do not hesitate to ask.

Gracias por contactarnos de nuevo.

¿Podrías por favor indicarme el número de pedido en el que se enviaron los anticuerpos? ¿En qué estado se recibieron? ¿Nada más recibirlos se alicuotaron y guardaron a -20C?

¿En qué aplicación y especies se están usando?

Te agradezco que nos facilites estos detalles para poder entender mejor qué ha podido ocurrir para que ninguno de los tres anticuerpos funcione.

Gracias por la colaboración. Quedo a la espera de tu respuesta.

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I hope this is helpful. Please contact me again with any technical questions you may have.

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If I understand you correctly. The anti-mouse IgG would recognize your purified normal mouse IgG or it would recognize Ab69. These, in turn, would be recognized by goat anti-mouse HRP. However if you are to use ab69 against HPV in mouse tissues your detection antibody (goat anti-mouse IgG-HRP) should IgG2a specific,as it may recognize both ab69 and the Fc receptors in the tissue thereby creating a false positive.

It seems that youare proposing a sandwich ELISA. These can be difficult to optimize. The antibodies must be detecting different epitopes of the antigen so they do not inferfere with the other antibody binding. We are unable to guarantee our antibodies in sandwich ELISA unless they have been specifically tested in that application.

If ab69 is your detection


I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Thank you for contacting Abcam. I have attached the MSDS for all except ab500, ChIP kit. I am still waiting for the laboratory to get back to me on that one and I will send it on as soon as I get it. If there is anything else I can do, please let me know.

Thank you for your inquiry. Both Anti-HPV16 L1 recognise HPV Type 16, L1 (major capsid protein). Unfortunately, we do not currently have more information about the epitopes for those monoclonal antibodies. We will update the on-line datasheet for this product as soon as we receive additional data. We apologize for any inconvenience this may cause.

Thank you for your email. We do not routinely offer free or trial sized samples for testing purposes. Our policy at Abcam is that if an antibody does not work as specified on the datasheet, we will offer a replacement or reimbursement. Should you decide to test an antibody in an application for which we do not have any information, please let us know how you get on and in return we will award you 100 points with the Abcam Loyalty Scheme which can be redeemed on a number of rewards. If you have any additional questions, please contact us again.