Anti-HPV16 L1 antibody [CamVir 1] (ab69)

Submit a review Q&A (13)References (15)

Key features and details

  • Mouse monoclonal [CamVir 1] to HPV16 L1
  • Suitable for: ELISA, WB, IP, IHC-P
  • Reacts with: Human papillomavirus
  • Isotype: IgG2a

Overview

  • Product name

    Anti-HPV16 L1 antibody [CamVir 1]
    See all HPV16 L1 primary antibodies

  • Description

    Mouse monoclonal [CamVir 1] to HPV16 L1

  • Host species

    Mouse

  • Specificity

    The antibody reacts with a 56 kDa protein in cells infected with L1-vaccinia virus, the protein being present in a predominantly nuclear location. The antibody reacts very strongly with biopsy specimins containing HPV-16 or -33; very weak reactions were occasionally observed with biopsy specimens or smears containing HPV-6 or HPV-11. Cross-reacts with HPV37.

  • Tested applications

    Suitable for: ELISA, WB, IP, IHC-Pmore details

  • Species reactivity

    Reacts with: Human papillomavirus

  • Immunogen

    Human papilloma virus type 16, major capsid protein L1.

Properties

Applications

Our Abpromise guarantee covers the use of ab69 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA Use at an assay dependent concentration. PubMed: 20600940
WB Use at an assay dependent concentration. Predicted molecular weight: 56 kDa.
IP Use at an assay dependent concentration.
IHC-P Use at an assay dependent concentration.

Target

  • Relevance

    L1 is a major capsid protein of type 16 human papilloma virus. Infection with specific types of HPV has been associated with an increased risk of developing cervical neoplasia. HPV types 6 and 11 have been associated with relatively benign diseases such as genital warts but types 16 and 18 are strongly associated with cervical, vaginal, and vulvar malignancies.

  • Cellular localization

    Virion. Host Nucleus.

  • Database links

    • Alternative names

      • HPV-16 antibody
      • HPV-16 capsid antibody
      • HPV16 capsid protein antibody
      • HPV16 L1 antibody
      • HPV16 major capsid protein L1 antibody
      • Human papillomavirus type 16 L1 antibody
      • Human papillomavirus type 16 major capsid protein L1 antibody
      • L1 antibody
      • Major capsid L1 protein antibody
      • Major capsid protein antibody
      • Major capsid protein L1 antibody
      see all

    References

    Publishing research using ab69? Please let us know so that we can cite the reference in this datasheet.

    ab69 has been referenced in 15 publications.

    • Xv Z  et al. Effective Neutralizing Antibody Produced in Mice Directly Immunized with Integrated Pichia pastoris Expressing HPV16L1 Protein. Viral Immunol 32:308-317 (2019). PubMed: 31381497
    • Day PM  et al. Interferon Gamma Prevents Infectious Entry of Human Papillomavirus 16 via an L2-Dependent Mechanism. J Virol 91:N/A (2017). WB . PubMed: 28250129
    • Grant BD  et al. A paper-based immunoassay to determine HPV vaccination status at the point-of-care. Vaccine 34:5656-5663 (2016). PubMed: 27667331
    • Lamprecht RL  et al. Production of Human papillomavirus pseudovirions in plants and their use in pseudovirion-based neutralisation assays in mammalian cells. Sci Rep 6:20431 (2016). PubMed: 26853456
    • Warren CJ  et al. The antiviral restriction factors IFITM1, 2 and 3 do not inhibit infection of human papillomavirus, cytomegalovirus and adenovirus. PLoS One 9:e96579 (2014). WB . PubMed: 24827144
    View all Publications for this product

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    1-10 of 13 Abreviews or Q&A

    Question

    Thank you very much for the information.
    I am however looking for the binding site of your camVir1 antibody. That is against HPV16 L1. Do you have a paper on that?
    Thanking you for your help

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    Answer

    Thank you for contacting us.

    Unfortunately, we do not have any other information about the binding site, I am afraid!. The CamVir1 clone is a published clone, please check the literature if anybody else have published any more details about the epitope.

    I hope this information is nevertheless helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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    Question

    Product code: 69
    Inquiry: Hi, Which epitope on L1 does camvir1 bind to? Thanks Inga

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    Answer

    Thank you for contacting us.

    The exact epitope sequence has been mapped. The immunogen is a gel-purified HPV-18 E6-b galactosidase fusion protein.

    Please check the link below of original publication;

    http://vir.sgmjournals.org/content/68/5/1351.full.pdf

    I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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    Question

    What is the epitope of antibody ab69?

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    Answer

    Thank you for contacting us.

    Please find attached the requested paper.

    I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

    Use our products? Submit an Abreview. Earn rewards!
    https://www.abcam.com/abreviews

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    Question

    Dear technical team,

    Our customer is going to purchase ab69 and she would like to know if it has cross-reactivity to other HPV types except type 16.
    Please let me know.

    Best regards

    Read More
    Answer

    Thank you for contacting us.

    The anti-HPV16 L1 antibody [CamVir 1] (ab69) has shown some cross reactivity with other HPV types other than 16.The antibody reacts very strongly with biopsy specimens containing HPV-16 or -33, whilstvery weak reactionshave occasionally observed with biopsy specimens or smears containing HPV-6 or HPV-11. There has also been cross reactivity observed with HPV37.

    I hope this information has been of help. If you have any other questions, please do not hesitate to ask.

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    Question

    Hola de nuevo Abcam:
    Uno de nuestros clientes adquirió estos 3 anticuerpos con nosotros, ellos los alicuotaron y almacenaron a -20°C, ahora que tratan de usarlos ninguno de los tres funciona, nos podrán ayudar, aún están dentro de los 6 meses de abpromise?
    AB10419 LOTE XXXXXXXX
    AB69 LOTE XXXXXXXXXX
    AB10505 LOTE XXXXXXXXX
    Muchas Gracias.¡

    Read More
    Answer

    Gracias por contactarnos de nuevo.

    ¿Podrías por favor indicarme el número de pedido en el que se enviaron los anticuerpos? ¿En qué estado se recibieron? ¿Nada más recibirlos se alicuotaron y guardaron a -20C?

    ¿En qué aplicación y especies se están usando?

    Te agradezco que nos facilites estos detalles para poder entender mejor qué ha podido ocurrir para que ninguno de los tres anticuerpos funcione.

    Gracias por la colaboración. Quedo a la espera de tu respuesta.

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    Question

    Thank you what is the status of our order?

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    Answer

    Unfortunately shipment of this order is being held up by our accounts team.

    Our accounting department can be contacted by email at mailto:us.credits@abcam.com or by telephone using the information at the Contact Us link in the top right corner of our website. Please refer to theorder reference numberin any correspondence with our accounting department.

    I hope this is helpful. Please contact me again with any technical questions you may have.

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    Question

    I purchased your mouse anti-HPV 16 L1 ab a while back. I am using the ab for ELISA purposes (to test levels of HPV specific IgG). I am trying to figure out appropriate standards for my ELISA. At first, I was thinking that I might coat a plate with anti-mouse IgG, titrate purified normal mouse IgG, and probe with a goat anti-mouse IgG-HRP Ab. But I wonder, if I coat with anti-mouse IgG, could I titrate with the Ab69 and pick up a signal after probing with goat anti-mouse IgG-HRP. I'm thinking the answer is no, but wanted to check. Could you help me figure out appropriate standards for using this antibody in ELISA format?

    Read More
    Answer

    Thank you for contacting us.


    If I understand you correctly. The anti-mouse IgG would recognize your purified normal mouse IgG or it would recognize Ab69. These, in turn, would be recognized by goat anti-mouse HRP. However if you are to use ab69 against HPV in mouse tissues your detection antibody (goat anti-mouse IgG-HRP) should IgG2a specific,as it may recognize both ab69 and the Fc receptors in the tissue thereby creating a false positive.

    It seems that youare proposing a sandwich ELISA. These can be difficult to optimize. The antibodies must be detecting different epitopes of the antigen so they do not inferfere with the other antibody binding. We are unable to guarantee our antibodies in sandwich ELISA unless they have been specifically tested in that application.

    If ab69 is your detection


    I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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    Question

    To Whom It May Concern:   I am requesting an MSDS (Material Safety Data Sheet) for a product used by a client of mine, Thunder Bay Regional Research.  The products are as follows:   -HPV16 L1 (product code: ab69) -HPV16 E2 (product code: ab17185) -mouse monoclonal to CD11C (BU15) (product code: ab11029) -Carbonic anhydrase 9 (product code: AB47011) -ChIP Kit (product code: AB500) -mouse monoclonal to HPV16 E6 and HPV 18 E6 (C1P5) (product code: ab70)   Are these products exempt from having an msds?  

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    Answer

    Thank you for contacting Abcam. I have attached the MSDS for all except ab500, ChIP kit. I am still waiting for the laboratory to get back to me on that one and I will send it on as soon as I get it. If there is anything else I can do, please let me know.

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    Question

    Dear technical team,   Our customer would like to know more information about immunogen of ab69 and ab30908.   If you have information of amino acid sequence, please let me know.   Thank you in advance.

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    Answer

    Thank you for your inquiry. Both Anti-HPV16 L1 recognise HPV Type 16, L1 (major capsid protein). Unfortunately, we do not currently have more information about the epitopes for those monoclonal antibodies. We will update the on-line datasheet for this product as soon as we receive additional data. We apologize for any inconvenience this may cause.

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    Question

    We routinely buy antibodies from Abcam, so I was wondering if you could send us a small amount of the ab69 HPV16-L1 (CamVir 1) antibody to test before we purchase a larger amount.

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    Answer

    Thank you for your email. We do not routinely offer free or trial sized samples for testing purposes. Our policy at Abcam is that if an antibody does not work as specified on the datasheet, we will offer a replacement or reimbursement. Should you decide to test an antibody in an application for which we do not have any information, please let us know how you get on and in return we will award you 100 points with the Abcam Loyalty Scheme which can be redeemed on a number of rewards. If you have any additional questions, please contact us again.

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    1-10 of 13 Abreviews or Q&A

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