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Microbiology Organism Virus DNA Virus double stranded DNA Virus Human Papillomavirus
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Recombinant

Recombinant Anti-HPV16 L1 antibody [rHPV16L1/1058] - BSA and Azide free (ab236304)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HPV16 L1 antibody [rHPV16L1/1058] - BSA and Azide free (ab236304)
  • Anti-HPV16 L1 antibody [rHPV16L1/1058] - BSA and Azide free (ab236304)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Mouse monoclonal [rHPV16L1/1058] to HPV16 L1 - BSA and Azide free
  • Suitable for: IHC-P
  • Reacts with: Human papillomavirus

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Protein
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Recombinant HPV16 L1 protein (ab119880)
Conjugation
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Alexa Fluor® 488 Conjugation Kit (Fast) - Lightning-Link® (ab236553)

View more associated products

Overview

  • Product name

    Anti-HPV16 L1 antibody [rHPV16L1/1058] - BSA and Azide free
    See all HPV16 L1 primary antibodies
  • Description

    Mouse monoclonal [rHPV16L1/1058] to HPV16 L1 - BSA and Azide free
  • Host species

    Mouse
  • Tested applications

    Suitable for: IHC-Pmore details
  • Species reactivity

    Reacts with: Human papillomavirus
  • Immunogen

    Full length protein corresponding to Human papillomavirus HPV16 L1. (Native protein).
    Database link: P03101

  • Positive control

    • IHC-P: Human cervix tissue.
  • General notes

     

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
  • Storage buffer

    Constituent: PBS
  • Carrier free

    Yes
  • Concentration information loading...
  • Purity

    Protein A/G purified
  • Purification notes

    Purified from Bioreactor Concentrate by Protein A/G.
  • Clonality

    Monoclonal
  • Clone number

    rHPV16L1/1058
  • Isotype

    IgG1
  • Research areas

    • Microbiology
    • Organism
    • Virus
    • DNA Virus
    • double stranded DNA Virus
    • Human Papillomavirus
    • Cancer
    • Oncoproteins/suppressors
    • Viral proteins
    • HPV
    • Microbiology
    • Protein
    • Viral Protein

Associated products

  • Alternative Versions

    • Anti-HPV16 L1 antibody [rHPV16L1/1058] (ab234309)
  • Compatible Secondaries

    • Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)
    • Goat Anti-Mouse IgG H&L (HRP) (ab205719)
  • Conjugation kits

    • FITC Conjugation Kit (Fast) - Lightning-Link® (ab188285)
  • Isotype control

    • Mouse IgG1, kappa monoclonal [15-6E10A7] - Isotype Control (ab170190)
  • Recombinant Protein

    • Recombinant HPV16 L1 protein (ab119880)
  • Related Products

    • Recombinant HPV16 L1 protein (ab119880)

Applications

Our Abpromise guarantee covers the use of ab236304 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 0.5 - 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Primary incubation for 30 minutes at room temperature

Target

  • Relevance

    L1 is a major capsid protein of type 16 human papilloma virus. Infection with specific types of HPV has been associated with an increased risk of developing cervical neoplasia. HPV types 6 and 11 have been associated with relatively benign diseases such as genital warts but types 16 and 18 are strongly associated with cervical, vaginal, and vulvar malignancies.
  • Cellular localization

    Virion. Host Nucleus.
  • Database links

    • Entrez Gene: 1489082 Human papillomavirus
    • SwissProt: P03101 Human papillomavirus
    • Alternative names

      • HPV-16 antibody
      • HPV-16 capsid antibody
      • HPV16 capsid protein antibody
      • HPV16 L1 antibody
      • HPV16 major capsid protein L1 antibody
      • Human papillomavirus type 16 L1 antibody
      • Human papillomavirus type 16 major capsid protein L1 antibody
      • L1 antibody
      • Major capsid L1 protein antibody
      • Major capsid protein antibody
      • Major capsid protein L1 antibody
      see all

    Images

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HPV16 L1 antibody [rHPV16L1/1058] - BSA and Azide free (ab236304)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HPV16 L1 antibody [rHPV16L1/1058] - BSA and Azide free (ab236304)

      Formalin-fixed, paraffin-embedded human cervix tissue stained for HPV16 L1 using ab234309 at 1 μg/ml in immunohistochemical analysis.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA and sodium azide (ab234309).

       

    • Anti-HPV16 L1 antibody [rHPV16L1/1058] - BSA and Azide free (ab236304)
      Anti-HPV16 L1 antibody [rHPV16L1/1058] - BSA and Azide free (ab236304)

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet
  • References (0)

    Publishing research using ab236304? Please let us know so that we can cite the reference in this datasheet.

    ab236304 has not yet been referenced specifically in any publications.

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